A circRNA–miRNA–mRNA network analysis underlying pathogenesis of human heart failure

BACKGROUND The molecular mechanisms of heart failure(HF) are still poorly understood. Circular RNA(circRNA) has been discovered in the heart in increasing numbers of studies. The goal of this research is to learn more about the potential roles of circRNAs in HF.METHODS & RESULTS We used RNA sequencing data to identify the characteristics of circRNAs expressed in the heart and discovered that the majority of circRNAs screened were less than 2000 nt. Additionally, chromosomes One and Y had the most and least number of circRNAs, respectively. After excluding duplicate host genes and intergenic circRNAs, a total of 238 differentially expressed circRNAs(DECs) and 203 host genes were discovered. However, only four of the 203 host genes of DECs were examined in HF differentially expressed genes. Another study used Gene Oncology analysis of DECs host genes to elucidate the underlying pathogenesis of HF, and it found that binding and catalytic activity accounted for a large portion of DECs. Immune system, metabolism, and signal transduction pathways were significantly enriched. Furthermore, 1052 potentially regulated miRNAs from the top 40 DECs were collected to build a circRNA-mi RNA network, and it was discovered that 470 miRNAs can be regulated by multiple circRNAs, while others are regulated by a single circRNA. In addition, a comparison of the top 10m RNAs in HF and their targeted miRNAs revealed that DDX3Y and UTY were regulated by the most and least circRNA, respectively.CONCLUSION These findings demonstrated circRNAs have species and tissue specific expression patterns;while circRNA expression is independent on host genes, the same types of genes in DECs and DEGs worked in HF. Our findings would contribute to a better understanding of the critical roles of circRNAs and lay the groundwork for future studies of HF molecular functions.

Nonlinear Optomechanically Induced Transparency in a Spinning Kerr Resonator

We theoretically study optomechanically induced transparency in a spinning Kerr-nonlinear resonator.The interplay between the optical Kerr effect and the Sagnac effect provides a flexible tool for modifying the optomechanically induced transparency windows of the signal comparing to the system of a single spinning resonator.It is found that the system will exhibit distinct transparency phenomenon and fast-to-slow light effects.More importantly,a symmetric transparency window appears by adjusting the rotation-induced Sagnac frequency shift to compensate for the Kerr-induced frequency shift.These results open up a new way to explore novel light propagation of optomechanically induced transparency devices in spinning resonators with Kerr nonlinearity.

Research of handwriting detecting system for space pen

A handwriting detecting system based on Micro- accelerometer and Micro-gyros is proposed. And the algorithm of the detecting system is also described in detail. And the error analysis of the detecting system is also described in de-tail. The motion contrail of the handwriting de-tecting in the 3-D space can be recognized through compute the matrix of attitude angles and the dynamic information of the handwriting detecting which is mapped on the 2-D plane. Then the information of contrail can be recurred on the writing plane by integral. There were good results in the actual experiment.

Subspace identification for continuous-time errors-in-variables model from sampled data

We study the subspace identification for the continuous-time errors-in-variables model from sampled data.First,the filtering approach is applied to handle the time-derivative problem inherent in continuous-time identification.The generalized Poisson moment functional is focused.A total least squares equation based on this filtering approach is derived.Inspired by the idea of discrete-time subspace identification based on principal component analysis,we develop two algorithms to deliver consistent estimates for the continuous-time errors-in-variables model by introducing two different instrumental variables.Order determination and other instrumental variables are discussed.The usefulness of the proposed algorithms is illustrated through numerical simulation.