Background： During the past decades, the incidence of invasive aspergillosis （IA） caused by Aspergillus funtigatus has increased dramatically. The aims of this study were to investigate the susceptibility of clinic...Background： During the past decades, the incidence of invasive aspergillosis （IA） caused by Aspergillus funtigatus has increased dramatically. The aims of this study were to investigate the susceptibility of clinical isolates of A.fumigatus to triazole and the underlying cyp51A mutations in triazole-resistant A. fumigatus. Methods： A total of 126 A.Jumigatus clinical isolates from 126 patients with proven or probable IA were obtained from four large tertiary hospitals in Nanjing, China, between August 2012 and July 2015. The determination of minimal inhibitory concentrations （MICs） for itraconazole, voriconazole, and posaconazole was performed by broth microdilution according to the European Committee on Antimicrobial Susceptibility Testing reference method. Results： A total of 4 A.fumigatus isolates （3.17%） were confinned to be itraconazole resistant, with MICs of ≥8 mg/L, and one isolate （0.8%） was confirmed to be voriconazole resistant and posaconazole resistant, with MICs of 4 mg/L and 0.5 mg/L, respectively. We found that two of the 4 isolates of triazole-resistant A. fumigatus had the L98H amino acid substitution in combination with a 34-base pair tandem repeat in the promoter region, one isolate had an M2201 mutation, and another itraconazole-resistant isolate did not have a substitution in the civp51A gene. Conclusions： This study shows that triazole-resistant A.fumigatus clinical isolates are present in Nanjing, China, which is a new challenge to the clinical management of IA.展开更多
文摘Background： During the past decades, the incidence of invasive aspergillosis （IA） caused by Aspergillus funtigatus has increased dramatically. The aims of this study were to investigate the susceptibility of clinical isolates of A.fumigatus to triazole and the underlying cyp51A mutations in triazole-resistant A. fumigatus. Methods： A total of 126 A.Jumigatus clinical isolates from 126 patients with proven or probable IA were obtained from four large tertiary hospitals in Nanjing, China, between August 2012 and July 2015. The determination of minimal inhibitory concentrations （MICs） for itraconazole, voriconazole, and posaconazole was performed by broth microdilution according to the European Committee on Antimicrobial Susceptibility Testing reference method. Results： A total of 4 A.fumigatus isolates （3.17%） were confinned to be itraconazole resistant, with MICs of ≥8 mg/L, and one isolate （0.8%） was confirmed to be voriconazole resistant and posaconazole resistant, with MICs of 4 mg/L and 0.5 mg/L, respectively. We found that two of the 4 isolates of triazole-resistant A. fumigatus had the L98H amino acid substitution in combination with a 34-base pair tandem repeat in the promoter region, one isolate had an M2201 mutation, and another itraconazole-resistant isolate did not have a substitution in the civp51A gene. Conclusions： This study shows that triazole-resistant A.fumigatus clinical isolates are present in Nanjing, China, which is a new challenge to the clinical management of IA.
