折叠顶压球囊与传统巩膜外加压治疗孔源性视网膜脱离的对比研究

目的:对折叠顶压球囊(FCB)与传统巩膜外加压术(SB)治疗孔源性视网膜脱离(RRD)的疗效及并发症进行对比研究。方法:回顾性分析2019-03/2022-04我院81例82眼RRD患者的临床资料,对比两种治疗方式下患者术后的视网膜复位率、最佳矫正视力和视网膜下积液吸收的疗效差异,以及术后不适、并发症的发生率。结果:术后FCB组视网膜复位率为96%,SB组复位率为92%,两组无差异(P>0.05)。两组波及黄斑患眼的最佳矫正视力手术前后比较均有差异(P<0.01)。两组均可促进患眼视网膜下积液吸收。FCB组手术时间为16.50(12.75,25.00)min, SB组手术时间为38.00(36.25,41.75)min(P<0.001)。FCB组患者术后眼睑肿胀不适发生率明显低于SB组(P<0.001),术后1d FCB组VAS疼痛评分1.00(0.00,2.00)分,SB组3.00(2.00,3.00)分(P<0.001)。结论:FCB治疗RRD是一种安全有效且可减轻患者痛苦的手术方法,相较于SB,手术时间明显缩短,患者术后不良反应轻。

Impact of Lycium Barbarum Polysaccharide and Danshensu on vascular endothelial growth factor in the process of retinal neovascularization of rabbit

AIM:To discuss the impact of Lycium Barbarum Polysaccharide (LBP) and Danshensu purified from Traditional Chinese Medicine (TCM) on vascular endothelial growth factor (VEGF) of rabbits with retinal neovascularization. METHODS:Forty rabbits were divided into normal control group, model control group, LBP group and Danshensu group. Animals in the normal control group were fed in the normal oxygen environment. Animals in the other three groups were put into the environment with 70% oxygen for 5 days in order to build the model of oxygen-induced vascular proliferation retinopathy. And then different TCM extract was injected into the abdominal cavities of these annimals. After 7 days, the VEGF content of in the serum of rabbit was measured by double antibody sandwich method. RESULTS:Data analysis indicated that VEGF content was as follows:Danshensu group was lower than model control group (12.92 ±3.84ng/L vs 19.32 ±4.15ng/L, P 【 0.05); LBP group and normal control group were lower than model control group (12.92±3.84ng/L, 9.26±1.61ng/L vs 19.32±4.15ng/L, P【0.01); total blood viscosity, plasma viscosity, cholesterol content, fibrinogen content and triacylglycerol content after peritoneal injection of LBP and Danshensu were obviously lower than before injection. CONCLUSION:TCM extract-LBP and Danshensu can prominently reduce the content of VEGF in the process of vascular proliferative retinopathy of rabbit; can prevent the occurrence of retinal microvascular disease by improving partial oxygen -deficient environment or affecting all kinds of new growth factor.

Identification of candidate piRNAs in the gonads of Paralichthys olivaceus (Japanese flounder)

Piwi-interacting RNA （piRNA） plays an important role in the gonadal development and maintenance of Teleostei. In this study, piRNA libraries derived from the adult gonads of Japanese flounder （Paralichthys ofivaceus） were generated using next-generation sequencing technology. Using zebrafish piRNAs as a reference, 5 865 unique candidate piRNAs were identified; 289 candidate piRNA clusters （PRCs） were generated from the above piRNAs. Among the isolated candidate PRCs, a total of 38 ovary-specific, 45 ovary-bias, 24 testis-specific, and 131 testis-bias PRCs were found. The relative expression levels of seven PRCs were validated through quantitative reverse transcription-polymerase chain reaction. The results of this study will help facilitate exploration of the development and maintenance of the phenotypic sex mechanism in P. olivaceus.

TGF-β1/SMAD SIGNALING PATHWAY MEDIATES p53-DEPENDENT APOPTOSIS IN HEPATOMA CELL LINES

Objective To determine whether transforming growth factor betal (TGF-β1)/Smad signaling pathway mediates p53-dependent apoptosis in hepatoma cell lines.Methods Three human hepatic carcinoma cell lines, HepG2, Huh-7, and Hep3B, were used in this study.TGF-β1-induced apoptosis in hepatic carcinoma cell lines was analyzed using TUNEL assay.For identifying the mechanism of apoptosis induced by TGF-β1, cell lines were transfected with a TGF-β1-inducible luciferase reportor plasmid containing Smad4 binding elements.After transfection, cells were treated with TGF-β1, then assayed for luciferase activity.Results The apoptosis rate of HepG2 cell lines (48.51%± 8.21%) was significantly higher than control ( 12.72%±2.18%, P<0.05).But TGF-β1 was not able to induce apoptosis of Huh-7 and Hep3B cell lines.The relative luciferase activity of TGF-β1-treated HepG2 cell lines (4.38) was significantly higher than control (1.00, P< 0.05).But the relative luciferase activity of TGF-β1-treated Huh-7 and Hep3B cell lines less increased compared with control.Conclusions HepG2 cells seem to be highly susceptible to TGF-β1-induced apoptosis compared with Hep3B and Huh-7 cell lines.Smad4 is a central mediator of TGF-β1 signaling transdution pathway.TGF-β1/Smad signaling pathway might mediate p53-dependent apoptosis in hepatoma cell lines.

Wetting behaviors of methanol,ethanol,and propanol on hydroxylated SiO2 substrate

Water molecules could form a liquid droplet on the water monolayer on a specific solid surface, which has been referred to as ‘‘ordered water monolayer that does not completely wet water'' at room temperature. In contrast to the water molecules, the family of alcohol molecules has the same OH polar head and various lengths of their hydrophobic nonpolar tail; the length of the hydrophobic tail can affect the hydrophobic effect. In this study, using molecular dynamics simulations, we investigated the wetting behaviors of methanol, ethanol, and propanol molecules adsorbed on a SiO_2 surface. The results showed that the methanol, ethanol, and propanol molecules could form an ordered monolayer on the SiO_2 surface and a droplet on top of this monolayer, with different contact angles. The differences in the contact angles were attributed to the differences in the interactions between the alcohol monolayer and droplet.

Molecular rotation-caused autocorrelation behaviors of thermal noise in water

The finite autocorrelation time of thermal noise is crucial to unidirectional transportation on the molecular scale.Therefore,it is important to understand the cause of the intrinsic picosecond autocorrelation time of thermal noise in water.In this work,we use molecular dynamics simulations to compare the autocorrelation behaviors of the thermal noise,hydrogen bonds,and molecular rotations found in water.We found that the intrinsic picosecond autocorrelation time for thermal noise is caused by finite molecular rotation relaxation,in which hydrogen bonds play the role of a bridge.Furthermore,the simulation results show that our method of calculating the autocorrelation of thermal noise,by observing the fiuctuating force on an oxygen atom of water,provides additional information about molecular rotations.Our findings may advance the understanding of the anomalous dynamic nanoscale behavior of particles,and the applications of terahertz technology in measuring the structural and dynamical information of molecules in solutions.

SCR-22 of pollen-dominant S haplotype class is recessive to SCR-44 of pollenrecessive S haplotype class in Brassica rapa

SCR/SP11 encodes the male determinant of recognition specificity of self-incompatibility(SI)in Brassica species and is sporophytically expressed in the anther tapetum.Based on dominance relationships in pollen and nucleotide sequence similarity,the S haplotypes in Brassica have been classified as class I or class II,with class-I S haplotypes being dominant over class-II S haplotypes.Here,we revealed that S-22 in B.rapa belonging to class I is recessive to class-II S-44 and class-I S-36 in pollen,whereas it is dominant over S-60,S-40,and S-29 based on pollination tests.SCR/SP11 of S-22(SCR-22)was sequenced,revealing that the deduced amino-acid sequence of SCR-22 has the longest C-terminal domain among the SCR/SP11 sequences.The expression of SCR-22 was found to be suppressed in S-22/S-44 and S-22/S-36 heterozygotes.Normal transcription of SCR-44 was considered to be due to the transcription suppression of Smi sRNA of the S-22 haplotype and a very low methylation state of the SCR-44 promoter region in the tapetum of S-22/S-44 heterozygotes.In SCR-22,only the cytosine residue located at the–37 bp position of the promoter region was hypermethylated in the tapetum of S-22/S-44 heterozygotes,and few methylated cytosines were detected in the promoter and coding regions of SCR-22 in S-22/S-36 heterozygotes.SCR-22 was also expressed in microspores in S-22 homozygotes but not in S-22/S-44 and S-22/S-36 heterozygotes.These results suggest that a mechanism different from class-II SCR/SP11 suppression may operate for the suppression of recessive class-I SCR-22 in S heterozygotes.

Prediction of high-mobility two-dimensional electron gas at KTaO_3-based heterointerfaces

First-principles calculations are performed to explore the possibility of generating the two-dimensional electron gas(2 DEG) at the interface between LaGaO_3/KTaO_3 and NdGaO_3/KTaO_3(001) heterostructures. Two different models —i.e., the superlattice model and the thin film model — are used to conduct a comprehensive investigation of the origin of charge carriers. For the symmetric superlattice model, the LaGaO_3(or NdGaO_3) film is nonpolar. The 2 DEG with carrier density on the order of 1014 cm^(-2) originates from the Ta dxy electrons contributed by both LaGaO_3(or NdGaO_3) and KTaO_3. For the thin film model, large polar distortions occur in the LaGaO_3 and NdGaO_3 layer, which entirely screens the built-in electric field and prevents electrons from transferring to the interface. Electrons of KTaO_3 are accumulated at the interface, contributing to the formation of the 2 DEG. All the heterostructures exhibit conducting properties regardless of the film thickness. Compared with the Ti dxy electrons in SrTiO_3-based heterostructures, the Ta dxy electrons have small effective mass and they are expected to move with higher mobility along the interface. These findings reveal the promising applications of 2 DEG in novel nanoelectronic devices.

Effects of Liraglutide on endothelial function, immune function and related factors in patients with type 2 diabetes mellitus

Objective: To investigate the effects of Liraglutide on endothelial function, immune function and related factors in patients with type 2 diabetes mellitus. Methods: A total of 160 patients with type 2 diabetes mellitus who received treatment in department of endocrinology of our hospital from December 2015 to June 2018 were collected. They were randomly divided into the control group and the observation group, 80 cases in each group. The patients in the control group were treated with metformin. The patients in the observation group were treated with liraglutide on the basis of the control group. The levels of endothelin (ET-1), nitric oxide (NO), immunoglobulin A (IgA), immunoglobulin M (IgM), immunoglobulin G (IgG), hypersensitive C-reactive protein (hs-CRP), insulin-like growth factor-1 (IGF-1), tumor necrosis factor- (TNF-α), Vaspin and HbAlc were detected and compared. Results: After treatment, compared with the treatment group, the serum levels of ET-1, hs-CRP, IGF-1, TNF-α and HbAlc of patients in the control group and the observation group were significantly decreased, and the levels of NO, IgA, IgG, IgM and Vaspin increased significantly. The change trend of the above indexes of patients in the observation group was more obvious than those in the control group (P<0.05). Conclusion: Liraglutide combined with metformin in the treatment of type 2 diabetes mellitus can significantly enhance endothelial function and immune function of patients, and alleviate inflammation, and it has good clinical efficacy.

Turnover of diacylglycerol kinase 4 by cytoplasmic acidification induces vacuole morphological change and nuclear DNA degradation in the early stage of pear self-incompatibility response

Pear has an S-RNase-based gametophytic selfincompatibility(SI)system.Nuclear DNA degradation is a typical feature of incompatible pollen tube death,and is among the many physiological functions of vacuoles.However,the specific changes that occur in vacuoles,as well as the associated regulatory mechanism in pear SI,are currently unclear.Although research in tobacco has shown that decreased activity of diacylglycerol kinase(DGK)results in the morphological change of pollen tube vacuole,whether DGK regulates the pollen tube vacuole of tree plants and whether it occurs in SI response,is currently unclear.We found that DGK activity is essential for pear pollen tube growth,and DGK4 regulates pollen tube vacuole morphology following its high expression and deposition at the tip and shank edge of the pollen tube of pear.Specifically,incompatible S-RNase may induce cytoplasmic acidification of the pollen tube by inhibiting V-ATPase V0 domain a1 subunit gene expression as early as 30 min after treatment,when the pollen tube is still alive.Cytoplasmic acidification induced by incompatible S-RNase results in reduced DGK4 abundance and deposition,leading to morphological change of the vacuole and fragmentation of nuclear DNA,which indicates that DGK4 is a key factor in pear SI response.

Multiple-valley effect on modulation of thermoelectric properties of n-type ZrCuSiAs-structure oxyantimonides LnTSbO(Ln=lanthanides and T=Zn,Mn)

Thermoelectric properties of n-type LnTSbO(Ln=lanthanides and T?Zn,Mn)were firstly investigated by the first-principles method and the semi-classical Boltzmann theory.The results show that a multiplevalley structure appears around the bottom of conduction band.The valley with a high band degeneracy consists of the bands with a weak band dispersion,leading to large magnitudes of the Seebeck coefficient but low electrical conductivity.The valley with a low band degeneracy is made up of the bands with an intense band dispersion,resulting in a high electrical conductivity but small magnitudes of the Seebeck coefficient.The thermoelectric properties are dominated by the energy difference,DE,between the valleys.The DE value of LnZnSbO linearly increases with the ionic radius of Ln.The thermoelectric properties are thus effectively modulated by varying the lanthanides.As a result,LnZnSbO(Ln?Ce-Nd)with the moderate values of DE shows a better thermoelectric performance.The multiple-valley effect is an effective way to modulate the thermoelectric properties of n-type LnTSbO.