Objective:To study the effect of rhizoma drynariae extract on osteoblast viability and proliferation as well as related gene expression. Methods:SD rats were selected, and the osteoblasts in the femur were isolated, c...Objective:To study the effect of rhizoma drynariae extract on osteoblast viability and proliferation as well as related gene expression. Methods:SD rats were selected, and the osteoblasts in the femur were isolated, cultured and divided into the rhizoma drynariae group that were treated with 0.02 g/mL rhizoma drynariae extract and the control group that were treated with serum-free medium;24 h, 36 h and 48 h after treatment, CCK-8 kits were used to determine osteoblast proliferation activity, and fluorescent quantitative PCR kits were used to determine the mRNA expression of osteogenic activity-related genes and proliferation-related genes.Results:24 h, 36 h and 48h after treatment, osteoblast proliferation activity of rhizoma drynariae group was significantly higher than that of control group;Runx2, OPN, OCN, ALP, OPG, PI3K, AKT, EKR1/2, CyclinD1, CDK2, CDK4 and E2F mRNA expression in osteoblasts of rhizoma drynariae group were significantly higher than those of control group. Conclusion:Rhizoma drynariae extract can promote osteoblast proliferation, increase the osteogenetic activity of osteoblasts and also accelerate the cell cycle process through the PI3K/AKT and ERK1/2 signaling pathways.展开更多
基金Natural Science Foundation of Inner Mongolia No:2013MS0752.
文摘Objective:To study the effect of rhizoma drynariae extract on osteoblast viability and proliferation as well as related gene expression. Methods:SD rats were selected, and the osteoblasts in the femur were isolated, cultured and divided into the rhizoma drynariae group that were treated with 0.02 g/mL rhizoma drynariae extract and the control group that were treated with serum-free medium;24 h, 36 h and 48 h after treatment, CCK-8 kits were used to determine osteoblast proliferation activity, and fluorescent quantitative PCR kits were used to determine the mRNA expression of osteogenic activity-related genes and proliferation-related genes.Results:24 h, 36 h and 48h after treatment, osteoblast proliferation activity of rhizoma drynariae group was significantly higher than that of control group;Runx2, OPN, OCN, ALP, OPG, PI3K, AKT, EKR1/2, CyclinD1, CDK2, CDK4 and E2F mRNA expression in osteoblasts of rhizoma drynariae group were significantly higher than those of control group. Conclusion:Rhizoma drynariae extract can promote osteoblast proliferation, increase the osteogenetic activity of osteoblasts and also accelerate the cell cycle process through the PI3K/AKT and ERK1/2 signaling pathways.
