Correlation between 25 hydroxyvitamin D3 and carotid atherosclerosis of newly diagnosed type 2 diabetes mellitus

Objective: To investigate the correlation between 25 hydroxyvitamin D3[25(OH)D3] and carotid atherosclerosis of newly diagnosed type 2 diabetes mellitus (T2DM). Methods: A total of 258 patients with newly diagnosed T2DM in this hospital between July 2015 and July 2017 were selected as T2DM group, and 100 subjects with normal glucose metabolism who received physical examination in this hospital during the same period were selected as normal control group. The differences in serum 25(OH)D3 levels, ultrasonic carotid atherosclerosis parameters as well as serum lipid metabolism index and inflammatory adipocytokines contents were compared between the two groups. Pearson test was used to assess the correlation between serum 25(OH)D3 level and carotid atherosclerosis in patients with newly diagnosed T2DM. Results: Serum 25(OH)D3 content of T2DM group was lower than that of normal control group;PWV and CC levels of the ultrasonic left and right carotid arteries were higher than those of normal control group;serum lipid metabolism indexes TC and LDL-C contents were higher than those of normal control group whereas HDL-C content was lower than that of normal control group;serum inflammatory cytokine APN content was lower than that of normal control group whereas CHEM, RSTN and LEP contents were higher than those of normal control group. Pearson test showed that serum 25(OH)D3 level in T2DM patients was directly correlated with the degree of carotid atherosclerosis. Conclusion: Serum 25(OH)D3 level is abnormally low in patients with newly diagnosed T2DM, and the specific decrease is directly correlated with the degree of carotid atherosclerosis and can be used as an early evaluation indicator for carotid artery disease in patients with T2DM.

Study on the relationship between PI3K/AKT signaling pathway and apoptosis in cartilage tissue of rats with osteoarthritis

Objective:To study the relationship between PI3K/AKT signaling pathway and apoptosis in cartilage tissue of rats with osteoarthritis (OA).Methods: The clean male Wistar rats were selected as experimental animals and randomly divided into OA model group and control group. The OA model was established by intra-articular injection of papain solution and L-cysteine. Fourth weeks and eighth weeks after model establishment, the expression of PI3K/AKT signaling molecules, inflammatory mediators, apoptosis marker molecules and autophagy marker molecules in articular cartilage were determined.Results: p-PI3K and p-AKT expression in articular cartilage of OA group were significantly lower than those of control group;IL-1β, IL-6, IL-17, IL-18, eIF4E, Bax, Caspase-3, mTOR, Beclin1, Atg5 and Atg7 expression in articular cartilage of OA group were significantly higher than those of control group and negatively correlated with p-PI3K and p-AKT expression while Bcl-2 expression in articular cartilage of OA group was significantly lower than that of control group and positively correlated with p-PI3K and p-AKT expression.Conclusion:The inhibition of PI3K/AKT signaling pathway in cartilage tissue of OA rat model can promote chondrocyte apoptosis and autophagy.