Yiqi Fusheng Decoction reverses cisplatin resistance in colorectal cancer by down-regulating HIF-1αand p-ERK1/2

Objective:To explore the reversing resistance mechanisms of Yiqi Fusheng Decoction(YQFS)on human multidrug-resistant colorectal cancer cell HT-29/DDP.Methods:Colorectal cancer cell HT-29/DDP was treated with YQFS for 48h and MTT assay was used to detect the effect of HT-29/DDP cells on the sensitivity of chemotherapeutic drugs.Cell apoptosis was measured by flow cytometry.The effects of YQFS on the expression of HIF-1α,ERK1/2 and p-ERK1/2 in HT-29/DDP cells were detected by Western blot.Results:High concentration and low concentration YQFS groups can significantly inhibit the proliferation of HT-29/DPP cells compared with the control group(P<0.01).And the high concentration YQFS group has a more significant inhibitory effect on cell proliferation.Compared with the cisplatin group,the proliferation of HT-29/DDP cells in YQFS and cisplatin group decreased dramatically and cell apoptosis increased significantly(P<0.01).Moreover,the expressions of HIF-1αand p-ERK were reduced in the YQFS group compared with the cisplatin group(P<0.01).Conclusion:YQFS can increase the sensitivity of multidrug-resistant colorectal cancer cells to chemotherapeutic drugs and promote cell apoptosis by down-regulating the expressions of HIF-1αand p-ERK1/2.