Improved mammalian family phylogeny using gap-rare multiple sequence alignment:A timetree of extant placentals and marsupials

The timing of mammalian diversification in relation to the Cretaceous-Paleogene(KPg)mass extinction continues to be a subject of substantial debate.Previous studies have either focused on limited taxonomic samples with available whole-genome data or relied on short sequence alignments coupled with extensive species samples.In the present study,we improved an existing dataset from the landmark study of Meredith et al.(2011)by filling in missing fragments and further generated another dataset containing 120 taxa and 98 exonic markers.Using these two datasets,we then constructed phylogenies for extant mammalian families,providing improved resolution of many conflicting relationships.Moreover,the timetrees generated,which were calibrated using appropriate molecular clock models and multiple fossil records,indicated that the interordinal diversification of placental mammals initiated before the Late Cretaceous period.Additionally,intraordinal diversification of both extant placental and marsupial lineages accelerated after the KPg boundary,supporting the hypothesis that the availability of numerous vacant ecological niches subsequent to the mass extinction event facilitated rapid diversification.Thus,our results support a scenario of placental radiation characterized by both basal cladogenesis and active interordinal divergences spanning from the Late Cretaceous into the Paleogene.

Relationship between acute kidney injury before thoracic endovascular aneurysm repair and in-hospital outcomes in patients with type B acute aortic dissection

ObjectiveAcute 肾损害(AKI ) 经常发生在基于导管的 interventional 过程和增加死亡以后。然而，类型 B 尖锐大动脉的解剖(AAD ) 的 AKI 以前胸的 endovascular 动脉瘤修理(TEVAR ) 的含意仍然保持不清楚。这研究与类型 B AAD.MethodsBetween 2009 在病人在 TEVAR 前评估了 AKI 的发生，预言者，和在里面医院结果并且 2013， 76 个病人回顾地被评估从症状发作在 36 h 以内为类型 B AAD 收到了 TEVAR。病人被分类进 no-AKI 对 AKI 组，并且 AKI 的严厉进一步根据肾疾病被上演：在外科手术前的 AKI 的 TEVAR.ResultsThe 发生前改进全球结果标准是 36.8% 。在里面医院复杂并发症与 no-AKI 相比在有外科手术前的 AKI 的病人是显著地更高的(50.0% 对 4.2% 分别地；P &#x0003c；0.001 ) ，包括尖锐肾的失败(21.4% 对 0 分别地；P &#x0003c；0.001 ) ，并且他们与 AKI 的严厉增加了(P &#x0003c；0.001 ) 。身体温度和白血房间计数的最大的层次是以前显著地与最大的浆液 creatinine 有关铺平 TEVAR。Multivariate 分析在承认上显示出那收缩血压(或：1.023；95% CI：1.003-1.044；P = 0.0238 ) 并且双边的肾的动脉参与(或：19.076；95% CI：1.914-190.164；P = 0.0120 ) 外科手术前的 AKI.ConclusionsPreoperative AKI 的强壮的预言者经常与类型 B AAD 在病人被发生，并且与更高的在里面医院复杂并发症相关并且提高了煽动性的反应。承认和双边的肾的动脉参与上的收缩血压是为在 TEVAR 前的 AKI 的主要风险因素。

Predictors and in-hospital outcomes of preoperative acute kidney injury in patients with type A acute aortic dissection

BackgroundAcute 肾损害(AKI ) 在为尖锐大动脉的解剖(AAD ) 的外科以后是普通的并且增加在里面医院和长期的死亡。然而，很少数据与类型在病人在早外科手术前的 AKI 的临床、预示的关联上存在 AAD。我们试图在病人在在里面医院结果上决定外科手术前的 AKI 的发生和 AKI 的影响与打阿德梅索兹夫罗姆·梅 2009 到 2014 年 6 月，我们回顾地注册了为类型从症状发作和收到的开的外科在 48 h 以内进入我们的医院的 178 个病人 AAD。病人被划分成没有 AKI 和 AKI 组并且在 surgery.ResultsAKI 发生在 41 个病人(23.0%) 以前，根据 KDIGO 标准与 AKI 上演了严厉。在里面医院复杂并发症的发生在有与没有 AKI 相比的外科手术前的 AKI 的病人是显著地更高的(41.5% 对 9.5% ， P <；0.001 ) ，包括肾的梗塞(7.3% 对 0， P = 0.012 ) ，并且它与 AKI 严厉增加了(P 趋势 <；0.001 ) 。尽管没有重要差别被发现，没有 AKI，有 AKI 的病人与病人相比有更高的在里面医院死亡(14.6% 对 5.1% ， P = 0.079 ) 。Multivariate 分析显示那男性，承认上的心脏舒张的血压和双边的肾的动脉参与 AAD.ConclusionsEarly 是在有类型 A 的病人的外科手术前的 AKI 的独立预言者在外科前的 AKI 在有类型的病人是普通的 AAD，并且与增加的在里面医院复杂并发症被联系。男性，承认上的心脏舒张的血压和双边的肾的动脉参与是为外科手术前的 AKI 的主要预言者。

Diagnostic value of HPV and P16 protein in patients with HSIL and prognosis

Objective: To investigate the diagnostic value and prognostic value of HPV and P16 protein in patients with HSIL and to provide a reference for the clinical diagnosis and assessment of the prognosis of patients with HSIL. Methods: The surgical treatment of HSIL patients from January 2013 to January 2015 in our hospital were selected. All patients were routinely tested for HPV and P16 protein, All patients were followed up for 1 year. Patients were divided into progressive group and quiescent group according to whether the disease progressed one year after surgery. Preoperative HPV and P16 protein levels were compared between the two groups. Using receiver operating curve (ROC curve) Analysis of HPV diagnostic value of HSIL. The levels of HPV and P16 protein in the two groups were analyzed and compared. Results: The quantitative level of high-risk HPV-DNA after LEEP was significantly lower than that before operation. The level of P16 protein in preoperative patients was higher than that before operation, and the difference was statistically significant. There were 21 patients in the postoperative progression group, and the average HPV-DNA content in the patients in the progression group was higher than that in the control group within one year after operation. The difference was statistically significant. The P16 protein level in patients in advanced group was significantly higher than that in resting group. Preoperative HPV-DNA levels and P16 protein levels in patients with progressive disease were significantly higher than those in still group. ROC curve analysis showed that the cut-off value of 2.441, HPV-DNA prediction of HSIL patients one year after the recurrence of the sensitivity was 95.12%, the specificity was 76.16%, under the curve area of 0.878;7.4 cut-off value, P16 The predictive value of HSIL patients recurrence after 1 year was 71.95%, specificity was 66.67%, and the area under the curve was 0.753. The recurrence group HPV-DNA content and P16 protein level showed a significant positive correlation, with statistical significance. Conclusions: LEEP can reduce the postoperative levels of HPV and P16 protein in patients with HSIL. The HPV and P16 protein levels are of high value for the early diagnosis of HSIL and the prediction of postoperative disease progression.