Three new amide alkaloids piperlongumamides A-C(1-3),together with 12 known ones(4-15),were isolated from the fruits of Piper longum.The structures of the new isolates were determined using spectroscopic data analyses...Three new amide alkaloids piperlongumamides A-C(1-3),together with 12 known ones(4-15),were isolated from the fruits of Piper longum.The structures of the new isolates were determined using spectroscopic data analyses.Cytotoxic activity of these amides against HL-60(human leukemia),A-549(human lung cancer),MCF-7(human breast cancer),SMMC-7721(human liver cancer)and SW480(human rectal cancer)cell lines were evaluated.Piperchabamide B(11)exhibited weak inhibitory activity against HL-60(IC_(50)=21.32μM),A-549(IC_(50)=23.82μM)and MCF-7(IC_(50)=16.58μM)cell lines.展开更多
Professor Heng Li(李恒,March 9,1929-January 12,2023)was a legendary plant taxonomist and phytogeographer at the Kunming Institute of Botany,Chinese Academy of Sciences(Fig.1).She made important contributions to our un...Professor Heng Li(李恒,March 9,1929-January 12,2023)was a legendary plant taxonomist and phytogeographer at the Kunming Institute of Botany,Chinese Academy of Sciences(Fig.1).She made important contributions to our understanding of China's flora,including the biodiversity and biogeography of Dulong Valley and the Gaoligong Mountains,as well as the vegetation of plateau lakes and wetlands in Southwest China.Professor Heng Li received her Bachelor's degree in Russian from Beijing Foreign Studies University in 1956 and immediately joined the Institute of Geography(now Institute of Geographic Sciences and Natural Resources Research),Chinese Academy of Sciences,as a Russian translator.In 1961,Prof.展开更多
Two new neolignans selaginellol(1)and selaginellol 4'-O-β-D-glucopyranoside(2),together with seven known compounds(3–9),were isolated from the whole plant of Selaginella moellendorffii.The structures of the new ...Two new neolignans selaginellol(1)and selaginellol 4'-O-β-D-glucopyranoside(2),together with seven known compounds(3–9),were isolated from the whole plant of Selaginella moellendorffii.The structures of the new isolates were determined through spectroscopic data analysis.Compounds 1–9,as well as compounds 10–18 previously isolated from the species,were measured for the activity against platelet aggregation induced by ADP or collagen.Three neoligans(8,11,and 12),one flavanone(14),and one alkaloid(16)showed inhibitory activity against ADP-or collagen-induced platelet aggregation as compared with tirofiban.The dihydrobenzofuran neolignans(8,11,and 12)are more potent than the benzofuran neolignan(13)and other types of neolignans(1–7).Glucosidation of the dihydrobenzofuran neolignans(11 and 12)is helpful for the activity.Graphical Abstract Two new neolignans selaginellol(1)and selaginellol 40-O-b-D-glucopyranoside(2)were isolated from the whole plant of Selaginella moellendorffii.Several compounds from this plant showed the activity against platelet aggregation induced by ADP or collagen.展开更多
One undescribedamide,pipermullesine A,twoundescribed isoquinoline alkaloids,pipermullesinesBand C,and six undescribed dipeptides,pipermullamides A–F,along with 28 known compounds,were isolated from the aerial parts o...One undescribedamide,pipermullesine A,twoundescribed isoquinoline alkaloids,pipermullesinesBand C,and six undescribed dipeptides,pipermullamides A–F,along with 28 known compounds,were isolated from the aerial parts of Piper mullesua.The structures of the undescribed compounds were elucidated based on the analysis of 1D and 2D NMR and MS data.Furthermore,the structures of pipermullesines A–Cwere confirmed by single crystal X-ray diffraction analysis.All isolates were evaluated for inhibitory activity against platelet aggregation induced by thrombin(IIa)or platelet-activating factor(PAF).(-)-Mangochinine,pellitorine,and(2E,4E)-N-isobutyl-2,4-dodecadienamide showed weak inhibitory activity against rabbit platelet aggregation induced by PAF,with IC_(50)values of 470.3μg/mL,614.9μg/mL,and 579.7μg/mL,respectively.展开更多
Objective:To clarify the active constituents of the heartwoods of Caesalpinia sappan,a traditional Chinese medicine with the functions of promoting blood circulation(Huoxue in Chinese)and removing blood stasis(Quyu in...Objective:To clarify the active constituents of the heartwoods of Caesalpinia sappan,a traditional Chinese medicine with the functions of promoting blood circulation(Huoxue in Chinese)and removing blood stasis(Quyu in Chinese).Methods:The chemical constituents were isolated and purified by combination of silica gel and Sephadex LH-20 column chromatography,along with semipreparative HPLC.Their chemical structures were established by multiple spectroscopic methods and comparison with literature data.The in vitro antiplatelet aggregation activities were evaluated using mouse platelet induced by AYPGKF-NH2,a gold agonist of protease-activated receptor 4(PAR4).Results:Two new phenols,methyl 2-(4,4’,5’-trihydroxy-2’-(methoxymethyl)biphenyl-2-yloxy)acetate(1)and 1’-methylcaesalpin J(2),together with 24 known compounds(3-26),were isolated from the heartwoods of C.sappan.Among them,sappanchalcone(16)and brazilin(20)showed inhibitory activities against mouse platelet aggregation with IC50 values of 114.8μmol/L and 100.8μmol/L,respectively.Conclusion:Antiplatelet compounds from C.sappan targeting at PAR4 are reported for the first time.展开更多
Edible fruits of Garcinia xanthochymus were traditionally used to treat diarrhoea and dysentery.Our previous work showed that some components of G.xanthochymus fruits induced apoptosis in colon cancer cells.In this st...Edible fruits of Garcinia xanthochymus were traditionally used to treat diarrhoea and dysentery.Our previous work showed that some components of G.xanthochymus fruits induced apoptosis in colon cancer cells.In this study,the aril(edible pulp of the fruit)and seed of G.xanthochymus fruits were further investigated for their展开更多
基金This work was funded by the Natural Science Foundation of Yunnan Province,China(No.2011FZ205)the National Natural Science Foundation of China(Nos.31070288,31161140345)+1 种基金the Ministry of Education of China through its 111 and 985 projects(Nos.B08044,MUC98506-01000101&MUC985-9)the Japan Society for the Promotion of Science(No.JSPS/AP/109080).
文摘Three new amide alkaloids piperlongumamides A-C(1-3),together with 12 known ones(4-15),were isolated from the fruits of Piper longum.The structures of the new isolates were determined using spectroscopic data analyses.Cytotoxic activity of these amides against HL-60(human leukemia),A-549(human lung cancer),MCF-7(human breast cancer),SMMC-7721(human liver cancer)and SW480(human rectal cancer)cell lines were evaluated.Piperchabamide B(11)exhibited weak inhibitory activity against HL-60(IC_(50)=21.32μM),A-549(IC_(50)=23.82μM)and MCF-7(IC_(50)=16.58μM)cell lines.
文摘Professor Heng Li(李恒,March 9,1929-January 12,2023)was a legendary plant taxonomist and phytogeographer at the Kunming Institute of Botany,Chinese Academy of Sciences(Fig.1).She made important contributions to our understanding of China's flora,including the biodiversity and biogeography of Dulong Valley and the Gaoligong Mountains,as well as the vegetation of plateau lakes and wetlands in Southwest China.Professor Heng Li received her Bachelor's degree in Russian from Beijing Foreign Studies University in 1956 and immediately joined the Institute of Geography(now Institute of Geographic Sciences and Natural Resources Research),Chinese Academy of Sciences,as a Russian translator.In 1961,Prof.
基金This work was funded by the Ministry of Science&Technology of China(2012FY110300)the National Natural Science Foundation of China(3116140345)the Minzu University of China(YLDX01013,2015MDTD16C).
文摘Two new neolignans selaginellol(1)and selaginellol 4'-O-β-D-glucopyranoside(2),together with seven known compounds(3–9),were isolated from the whole plant of Selaginella moellendorffii.The structures of the new isolates were determined through spectroscopic data analysis.Compounds 1–9,as well as compounds 10–18 previously isolated from the species,were measured for the activity against platelet aggregation induced by ADP or collagen.Three neoligans(8,11,and 12),one flavanone(14),and one alkaloid(16)showed inhibitory activity against ADP-or collagen-induced platelet aggregation as compared with tirofiban.The dihydrobenzofuran neolignans(8,11,and 12)are more potent than the benzofuran neolignan(13)and other types of neolignans(1–7).Glucosidation of the dihydrobenzofuran neolignans(11 and 12)is helpful for the activity.Graphical Abstract Two new neolignans selaginellol(1)and selaginellol 40-O-b-D-glucopyranoside(2)were isolated from the whole plant of Selaginella moellendorffii.Several compounds from this plant showed the activity against platelet aggregation induced by ADP or collagen.
基金This work was funded by the Southeast Asia Biodiversity Research Institute,Chinese Academy of Sciences(Y4ZK111B01)the Natural Science Foundation of Yunnan Province,China(2011FZ205)+2 种基金the International Partneship Program of Chinese Academy of Sciences(153631KYSB20160004)the Key Laboratory of Ethnomedicine(Minzu University of China)of Ministry of Education of China(KLEM-ZZ201806)the National Natural Science Foundation of China(31761143001&31161140345).
文摘One undescribedamide,pipermullesine A,twoundescribed isoquinoline alkaloids,pipermullesinesBand C,and six undescribed dipeptides,pipermullamides A–F,along with 28 known compounds,were isolated from the aerial parts of Piper mullesua.The structures of the undescribed compounds were elucidated based on the analysis of 1D and 2D NMR and MS data.Furthermore,the structures of pipermullesines A–Cwere confirmed by single crystal X-ray diffraction analysis.All isolates were evaluated for inhibitory activity against platelet aggregation induced by thrombin(IIa)or platelet-activating factor(PAF).(-)-Mangochinine,pellitorine,and(2E,4E)-N-isobutyl-2,4-dodecadienamide showed weak inhibitory activity against rabbit platelet aggregation induced by PAF,with IC_(50)values of 470.3μg/mL,614.9μg/mL,and 579.7μg/mL,respectively.
基金supported by the International Partnership Program of Chinese Academy of Sciences(153631KYSB20160004)the Southeast Asia Biodiversity Research Institute,Chinese Academy of Sciences(Y4ZK111B01).
文摘Objective:To clarify the active constituents of the heartwoods of Caesalpinia sappan,a traditional Chinese medicine with the functions of promoting blood circulation(Huoxue in Chinese)and removing blood stasis(Quyu in Chinese).Methods:The chemical constituents were isolated and purified by combination of silica gel and Sephadex LH-20 column chromatography,along with semipreparative HPLC.Their chemical structures were established by multiple spectroscopic methods and comparison with literature data.The in vitro antiplatelet aggregation activities were evaluated using mouse platelet induced by AYPGKF-NH2,a gold agonist of protease-activated receptor 4(PAR4).Results:Two new phenols,methyl 2-(4,4’,5’-trihydroxy-2’-(methoxymethyl)biphenyl-2-yloxy)acetate(1)and 1’-methylcaesalpin J(2),together with 24 known compounds(3-26),were isolated from the heartwoods of C.sappan.Among them,sappanchalcone(16)and brazilin(20)showed inhibitory activities against mouse platelet aggregation with IC50 values of 114.8μmol/L and 100.8μmol/L,respectively.Conclusion:Antiplatelet compounds from C.sappan targeting at PAR4 are reported for the first time.
文摘Edible fruits of Garcinia xanthochymus were traditionally used to treat diarrhoea and dysentery.Our previous work showed that some components of G.xanthochymus fruits induced apoptosis in colon cancer cells.In this study,the aril(edible pulp of the fruit)and seed of G.xanthochymus fruits were further investigated for their