Background:Sorting nexins are a large family of proteins that are associated with various components of the endosome system and they play many roles in processes such as endocytosis,intracellular protein trafficking a...Background:Sorting nexins are a large family of proteins that are associated with various components of the endosome system and they play many roles in processes such as endocytosis,intracellular protein trafficking and cell signaling.The subcellular distribution patterns of many of them remain controversial and their in vivo functions have not been characterized yet.Results:We investigated the subcellular distribution and function of SNX16 in this study.SNX16 is detected on Rab5-positive endosomes localized adjacent to focal adhesions at cell cortex.Inhibition of SNX23,polymerization of microtubule filaments as well as the PI3-kinase all disrupt the cell cortex distribution of SNX16.Ectopic expression of SNX16 reduces the migration and the tumor formation activity of MCF-7 cells.Conclusion:Our results indicate that,in addition to the PI3P,there is a SNX23-and microtubule-dependent cargo transport pathway required for the proper subcellular distribution of SNX16.SNX16 plays a negative regulatory role during cell migration and tumorigenesis.展开更多
To the Editor:The clinical spectrum of coronavirus disease 2019(COVID-19)appears to be wide,ranging from asymptomatic to severe progressive pneumonia with respiratory failure,multiorgan failure,and even death.Here,we ...To the Editor:The clinical spectrum of coronavirus disease 2019(COVID-19)appears to be wide,ranging from asymptomatic to severe progressive pneumonia with respiratory failure,multiorgan failure,and even death.Here,we investigated the clinical and pathological characteristics of a patient who died from severe COVID-19.Our findings will facilitate a deeper understanding of the pathogenesis and progression of COVID-19 and improve clinical strategies to combat the disease.On January 23,2020,a 57-year-old man without relevant history presented with fatigue and fever after attending a family party 2 days prior.Among the members of the family party,one relative had traveled from Wuhan.Over 9 days,the fever and cough developed,and the patient visited the emergency department.A throat swab was positive for severe acute respiratory syndrome coronavirus 2(SARS-COV-2)on real-time reverse-transcription polymerase chain reaction.He was admitted to the local hospital and received supportive therapies,anti-viral agents,atomized inhalation of interferon-γ,and oxygen therapy.Cough,fever,and dyspnea further developed on February 8.He received non-invasive mechanical ventilation therapy.On February 18,he was transferred to a superior hospital with shortness of breath.展开更多
基金We thank members of the lab for technical assistance and helpful discussions.This work was supported by grants from the"Strategic Priority Research Program"of the Chinese Academy of Sciences(XDA01020401,XDA01020307)National Natural Science Foundation of China(31271502)Science and Technology Planning Project of Guangdong Province(2011A060901019).
文摘Background:Sorting nexins are a large family of proteins that are associated with various components of the endosome system and they play many roles in processes such as endocytosis,intracellular protein trafficking and cell signaling.The subcellular distribution patterns of many of them remain controversial and their in vivo functions have not been characterized yet.Results:We investigated the subcellular distribution and function of SNX16 in this study.SNX16 is detected on Rab5-positive endosomes localized adjacent to focal adhesions at cell cortex.Inhibition of SNX23,polymerization of microtubule filaments as well as the PI3-kinase all disrupt the cell cortex distribution of SNX16.Ectopic expression of SNX16 reduces the migration and the tumor formation activity of MCF-7 cells.Conclusion:Our results indicate that,in addition to the PI3P,there is a SNX23-and microtubule-dependent cargo transport pathway required for the proper subcellular distribution of SNX16.SNX16 plays a negative regulatory role during cell migration and tumorigenesis.
基金This work was supported by grants from the National Natural Science Foundation of China(Nos.81770276,81772045,and 81902000)Novel coronavirus pneumonia emergency treatment and diagnosis technology research project of Heilongjiang Provincial Science and Technology Department,Nn10 program of Harbin Medical University Cancer Hospital and Scientific research project of Heilongjiang health and Family Planning Commission(No.2018086)。
文摘To the Editor:The clinical spectrum of coronavirus disease 2019(COVID-19)appears to be wide,ranging from asymptomatic to severe progressive pneumonia with respiratory failure,multiorgan failure,and even death.Here,we investigated the clinical and pathological characteristics of a patient who died from severe COVID-19.Our findings will facilitate a deeper understanding of the pathogenesis and progression of COVID-19 and improve clinical strategies to combat the disease.On January 23,2020,a 57-year-old man without relevant history presented with fatigue and fever after attending a family party 2 days prior.Among the members of the family party,one relative had traveled from Wuhan.Over 9 days,the fever and cough developed,and the patient visited the emergency department.A throat swab was positive for severe acute respiratory syndrome coronavirus 2(SARS-COV-2)on real-time reverse-transcription polymerase chain reaction.He was admitted to the local hospital and received supportive therapies,anti-viral agents,atomized inhalation of interferon-γ,and oxygen therapy.Cough,fever,and dyspnea further developed on February 8.He received non-invasive mechanical ventilation therapy.On February 18,he was transferred to a superior hospital with shortness of breath.
