The purpose of this study is to reveal the genetic mechanism of the variation of amylose content among different semi waxy or glutinous japonica rice in the background of Wxmp gene.Sixty-four semi waxy lines derived f...The purpose of this study is to reveal the genetic mechanism of the variation of amylose content among different semi waxy or glutinous japonica rice in the background of Wxmp gene.Sixty-four semi waxy lines derived from the hybrid progenies of Wujing 13 and Milky Princess(Kantou 194)with polymorphism in soluble starch synthase gene SSIIa(SSII-3)and SSIIIa(SSIII-2)but no polymorphism in other starch synthase related genes were used as test materials.The genotypes of SSIIa and SSIIIa allele were identified by molecular markers,and the allelic effects of SSIIa and SSIIIa gene on amylose content(AC),gel consistency(GC),gelatinization temperature(GT)and rapid visco analyzer(RVA)profile characteristics were analyzed.The significant effects of SSIIa and SSIIIa alleles and the interactive effects between two genes on AC,GT,GC and RVA profile characteristics were found.The SSIIa and SSIIIa alleles from Wujing13 shown positive effects on AC with an average increase of 1.87 and 1.23%in 2 years respectively.There was no significant effect on GT for SSIIa or SSIIIa allele but remarkable influence on GT when the co-existence of the two genes.The genotype SSIIa^(mp)SSIIIa^(mp) shown 1.34℃higher GT than genotype SSIIawjSSIIIawj(mp and wj indicated that the gene was derived from Milky Princess and Wujing 13 respectively,the same as in the below).Different genes and alleles resulted in significant different GC.The genetic effect of SSIIawj and SSIIIamp on GC was 8.74 and 9.62mm respectively.The GC of SSIIa^(wj)SSIIIa^(mp) was 10.64 and 16.95mm higher than that of SSIIa^(mp)SSIIIa^(wj) and SSIIawjSSIIIawj,respectively.The allele SSIIa^(wj) could increase the peak viscosity(PKV),hot paste viscosity(HPV),cool paste viscosity(CPV)and breakdown viscosity(BDV),while decrease the consistency viscosity(CSV)and setback viscosity(SBV).However for the allele SSIIIa^(wj) the opposite was true.The genotype SSIIa^(wj)SSIIIa^(mp) had the largest PKV,HPV and CPV,the genotype SSIIa^(wj)SSIIIa^(wj) had the largest BDV and CSV,but the genotype SSIIa^(wj)SSIIIa^(mp) had the least SBV.According to the comprehensive effect of each trait,the genotype SSIIawjSSIIIamp was the best.The allelic variation and interaction effect of SSIIa and SSIIIa genes have important reference value for improving cooking and eating quality of semi waxy japonica rice.展开更多
To understand rice types that were utilized during postdomestication and in the modern age and the potential of genetic research in aged rice materials,archaeogenetic analysis was conducted for two populations of arch...To understand rice types that were utilized during postdomestication and in the modern age and the potential of genetic research in aged rice materials,archaeogenetic analysis was conducted for two populations of archaic rice grains from the Mojiaoshan site during the Liangzhu Period in China(2940 to 2840 BC).Sequencing after the PCR amplification of three regions of the chloroplast genome and one region of the nuclear genome showed recovery rates that were comparable to those in previous studies except for one chloroplast genome region,suggesting that the materials used in this work were appropriate for recovering genetic information related to domestication traits by using advanced technology.Classification after sequencing in these regions proved the existence of Japonica and Indica chloroplasts in archaic grains from the west trench,which were subsequently classified into eight plastid groups(type I–VIII),and indicated that these rice grains derived from different maternal lineages were stored together in storage houses at the Mojiaohsan site.Among these plastid groups,type V exhibited the same sequences as two modern Indica accessions that are utilized in basic studies and rice breeding.It was inferred that part of the chloroplast genome of archaic rice has been preserved in modern genetic resources in these two modern Indica accessions,and the results indicated that rice related to their maternal ancestor was present at the Mojiaoshan site during the Liangzhu Period in China.The usefulness of archaeogenetic analysis can be demonstrated by our research data as well as previous studies,providing encouragement for the possibility that archaeogenetic analysis can be applied to older rice materials that were utilized in the rice-domesticated period.展开更多
基金supported by Jiangsu Natural Science Foundation(No.BK20180302)Jiangsu agricultural science and Technology Innovation Fund(No.CX[18]1001)+3 种基金Jiangsu Key Research and development program(No.BE2018357)Jiangsu major new varieties creation project(No.PZCZ201703)the earmarked fund for China Agriculture Research System(No.CARS-1-62)the open project of key Laboratory of Jiangsu crop genomics and molecular Breeding(No.PL201902).
文摘The purpose of this study is to reveal the genetic mechanism of the variation of amylose content among different semi waxy or glutinous japonica rice in the background of Wxmp gene.Sixty-four semi waxy lines derived from the hybrid progenies of Wujing 13 and Milky Princess(Kantou 194)with polymorphism in soluble starch synthase gene SSIIa(SSII-3)and SSIIIa(SSIII-2)but no polymorphism in other starch synthase related genes were used as test materials.The genotypes of SSIIa and SSIIIa allele were identified by molecular markers,and the allelic effects of SSIIa and SSIIIa gene on amylose content(AC),gel consistency(GC),gelatinization temperature(GT)and rapid visco analyzer(RVA)profile characteristics were analyzed.The significant effects of SSIIa and SSIIIa alleles and the interactive effects between two genes on AC,GT,GC and RVA profile characteristics were found.The SSIIa and SSIIIa alleles from Wujing13 shown positive effects on AC with an average increase of 1.87 and 1.23%in 2 years respectively.There was no significant effect on GT for SSIIa or SSIIIa allele but remarkable influence on GT when the co-existence of the two genes.The genotype SSIIa^(mp)SSIIIa^(mp) shown 1.34℃higher GT than genotype SSIIawjSSIIIawj(mp and wj indicated that the gene was derived from Milky Princess and Wujing 13 respectively,the same as in the below).Different genes and alleles resulted in significant different GC.The genetic effect of SSIIawj and SSIIIamp on GC was 8.74 and 9.62mm respectively.The GC of SSIIa^(wj)SSIIIa^(mp) was 10.64 and 16.95mm higher than that of SSIIa^(mp)SSIIIa^(wj) and SSIIawjSSIIIawj,respectively.The allele SSIIa^(wj) could increase the peak viscosity(PKV),hot paste viscosity(HPV),cool paste viscosity(CPV)and breakdown viscosity(BDV),while decrease the consistency viscosity(CSV)and setback viscosity(SBV).However for the allele SSIIIa^(wj) the opposite was true.The genotype SSIIa^(wj)SSIIIa^(mp) had the largest PKV,HPV and CPV,the genotype SSIIa^(wj)SSIIIa^(wj) had the largest BDV and CSV,but the genotype SSIIa^(wj)SSIIIa^(mp) had the least SBV.According to the comprehensive effect of each trait,the genotype SSIIawjSSIIIamp was the best.The allelic variation and interaction effect of SSIIa and SSIIIa genes have important reference value for improving cooking and eating quality of semi waxy japonica rice.
基金supported by a Scientific Research on Innovative Areas of the JSPS Grant-in-Aid(No.15H05968).
文摘To understand rice types that were utilized during postdomestication and in the modern age and the potential of genetic research in aged rice materials,archaeogenetic analysis was conducted for two populations of archaic rice grains from the Mojiaoshan site during the Liangzhu Period in China(2940 to 2840 BC).Sequencing after the PCR amplification of three regions of the chloroplast genome and one region of the nuclear genome showed recovery rates that were comparable to those in previous studies except for one chloroplast genome region,suggesting that the materials used in this work were appropriate for recovering genetic information related to domestication traits by using advanced technology.Classification after sequencing in these regions proved the existence of Japonica and Indica chloroplasts in archaic grains from the west trench,which were subsequently classified into eight plastid groups(type I–VIII),and indicated that these rice grains derived from different maternal lineages were stored together in storage houses at the Mojiaohsan site.Among these plastid groups,type V exhibited the same sequences as two modern Indica accessions that are utilized in basic studies and rice breeding.It was inferred that part of the chloroplast genome of archaic rice has been preserved in modern genetic resources in these two modern Indica accessions,and the results indicated that rice related to their maternal ancestor was present at the Mojiaoshan site during the Liangzhu Period in China.The usefulness of archaeogenetic analysis can be demonstrated by our research data as well as previous studies,providing encouragement for the possibility that archaeogenetic analysis can be applied to older rice materials that were utilized in the rice-domesticated period.