Rotator cuff(RC)attaches to humerus across a triphasic yet continuous tissue zones(bone-fibrocartilage-tendon),termed“enthesis”.Regrettably,rapid and functional enthesis regeneration is challenging after RC tear.The...Rotator cuff(RC)attaches to humerus across a triphasic yet continuous tissue zones(bone-fibrocartilage-tendon),termed“enthesis”.Regrettably,rapid and functional enthesis regeneration is challenging after RC tear.The existing grafts bioengineered for RC repair are insufficient,as they were engineered by a scaffold that did not mimic normal enthesis in morphology,composition,and tensile property,meanwhile cannot simultaneously stimulate the formation of bone-fibrocartilage-tendon tissues.Herein,an optimized decellularization approach based on a vacuum aspiration device(VAD)was developed to fabricate a book-shaped decellularized enthesis matrix(O-BDEM).Then,three recombinant growth factors(CBP-GFs)capable of binding collagen were synthesized by fusing a collagen-binding peptide(CBP)into the N-terminal of BMP-2,TGF-β3,or GDF-7,and zone-specifically tethered to the collagen of O-BDEM to fabricate a novel scaffold(CBP-GFs/O-BDEM)satisfying the above-mentioned requirements.After ensuring the low immunogenicity of CBP-GFs/O-BDEM by a novel single-cell mass cytometry in a mouse model,we interleaved urine-derived stem cell-sheets into this CBP-GFs/O-BDEM to bioengineer an enthesis-like graft.Its high-performance on regenerating enthesis was determined in a canine model.These findings indicate this CBP-GFs/O-BDEM may be an excellent scaffold for constructing enthesis-like graft to patch large/massive RC tears,and provide breakthroughs in fabricating graded interfacial tissue.展开更多
Precast concrete structures have developed rapidly in the last decades due to the advantages of better quality,non-pollution and fast construction with respect to conventional cast-in-place structures.In the present s...Precast concrete structures have developed rapidly in the last decades due to the advantages of better quality,non-pollution and fast construction with respect to conventional cast-in-place structures.In the present study,a theoretical model and nonlinear 3D model are developed and established to assess the dynamic behavior of precast concrete slabs under blast load.At first,the 3D model is validated by an experiment performed by other researchers.The verified model is adopted to investigate the blast performance of fabricated concrete panels(FCPs)in terms of parameters of the explosive charge,panel thickness,and reinforcement ratio.Finally,a simplified theoretical model of the FCP under blast load is developed to predict the maximum deflection.It is indicated that the theoretical model can precisely predict the maximum displacement of FCP under blast loads.The results show that the failure modes of the panels varied from bending failure to shear failure with the mass of TNT increasing.The thickness of the panel,reinforcement ratio,and explosive charges have significant effects on the anti-blast capacity of the FCPs.展开更多
基金supported by the National Natural Science Foundation of China(Nos.81902192 and 81730068)the Science and Technology Major Project of Changsha(No.kh2003015)+1 种基金the Postdoctoral Science Foundation of China(No.2019M652809)Additionally,we thank the staffs at BL01B station of National Facility for Protein Science Shanghai and the BL15U1 station of the Shanghai Synchrotron Radiation Facility,Shanghai,China,for their kind assistance during the experiments.
文摘Rotator cuff(RC)attaches to humerus across a triphasic yet continuous tissue zones(bone-fibrocartilage-tendon),termed“enthesis”.Regrettably,rapid and functional enthesis regeneration is challenging after RC tear.The existing grafts bioengineered for RC repair are insufficient,as they were engineered by a scaffold that did not mimic normal enthesis in morphology,composition,and tensile property,meanwhile cannot simultaneously stimulate the formation of bone-fibrocartilage-tendon tissues.Herein,an optimized decellularization approach based on a vacuum aspiration device(VAD)was developed to fabricate a book-shaped decellularized enthesis matrix(O-BDEM).Then,three recombinant growth factors(CBP-GFs)capable of binding collagen were synthesized by fusing a collagen-binding peptide(CBP)into the N-terminal of BMP-2,TGF-β3,or GDF-7,and zone-specifically tethered to the collagen of O-BDEM to fabricate a novel scaffold(CBP-GFs/O-BDEM)satisfying the above-mentioned requirements.After ensuring the low immunogenicity of CBP-GFs/O-BDEM by a novel single-cell mass cytometry in a mouse model,we interleaved urine-derived stem cell-sheets into this CBP-GFs/O-BDEM to bioengineer an enthesis-like graft.Its high-performance on regenerating enthesis was determined in a canine model.These findings indicate this CBP-GFs/O-BDEM may be an excellent scaffold for constructing enthesis-like graft to patch large/massive RC tears,and provide breakthroughs in fabricating graded interfacial tissue.
基金This work was supported by the National Natural Science Foundation of China(Grant No.51508148)China Postdoctoral Science Foundation Funded Project(Nos.2016T90563 and 2015M581980)+1 种基金open fund of Anhui Key Laboratory of Civil Engineering Structures and Materials in Hefei University of Technology,and State Key Laboratory of Structural Analysis for Industrial Equipment(Grant No.GZ19106)The authors also highly ppreciate the acknowledgment of the China Scholarship Council(CSC).
文摘Precast concrete structures have developed rapidly in the last decades due to the advantages of better quality,non-pollution and fast construction with respect to conventional cast-in-place structures.In the present study,a theoretical model and nonlinear 3D model are developed and established to assess the dynamic behavior of precast concrete slabs under blast load.At first,the 3D model is validated by an experiment performed by other researchers.The verified model is adopted to investigate the blast performance of fabricated concrete panels(FCPs)in terms of parameters of the explosive charge,panel thickness,and reinforcement ratio.Finally,a simplified theoretical model of the FCP under blast load is developed to predict the maximum deflection.It is indicated that the theoretical model can precisely predict the maximum displacement of FCP under blast loads.The results show that the failure modes of the panels varied from bending failure to shear failure with the mass of TNT increasing.The thickness of the panel,reinforcement ratio,and explosive charges have significant effects on the anti-blast capacity of the FCPs.
