Background:Our national collaborative research initiative is proposing to develop a common infrastructure for Rb research.We are proposing a novel in vivo Rb model using human Rb cells line.Methods:The rabbit model ha...Background:Our national collaborative research initiative is proposing to develop a common infrastructure for Rb research.We are proposing a novel in vivo Rb model using human Rb cells line.Methods:The rabbit model has advantages over the mouse models:(I)the larger eye size of rabbits,similar to the human infant eye,permits a more accurate injection of the drugs and evaluation of methods of targeted intraocular drug delivery;(II)the rabbit model demonstrated similar fundus appearance and pathologic features to human Rb,including vitreous seeds of viable tumor when the retinal tumor is mid-sized,which are usually found in the late stage in mouse models.The lack of ability to eliminate vitreous seeds is a major reason of current treatment failures in Group C and D tumors;therefore,the rabbit model of Rb may be used as a model to evaluate the effectiveness and various routes of drug delivery.Results:This is an implementation of an infrastructure for evaluating therapeutic targets.In addition,this finding enables a variety of pharmacokinetic studies,pharmacodynamic and toxicology studies for new therapeutic agents.Conclusions:This infrastructure meets the growing concern of practitioners and researchers in the field.The common facility is easily accessible to all VHRN members on request,including requests from other sectors.展开更多
文摘Background:Our national collaborative research initiative is proposing to develop a common infrastructure for Rb research.We are proposing a novel in vivo Rb model using human Rb cells line.Methods:The rabbit model has advantages over the mouse models:(I)the larger eye size of rabbits,similar to the human infant eye,permits a more accurate injection of the drugs and evaluation of methods of targeted intraocular drug delivery;(II)the rabbit model demonstrated similar fundus appearance and pathologic features to human Rb,including vitreous seeds of viable tumor when the retinal tumor is mid-sized,which are usually found in the late stage in mouse models.The lack of ability to eliminate vitreous seeds is a major reason of current treatment failures in Group C and D tumors;therefore,the rabbit model of Rb may be used as a model to evaluate the effectiveness and various routes of drug delivery.Results:This is an implementation of an infrastructure for evaluating therapeutic targets.In addition,this finding enables a variety of pharmacokinetic studies,pharmacodynamic and toxicology studies for new therapeutic agents.Conclusions:This infrastructure meets the growing concern of practitioners and researchers in the field.The common facility is easily accessible to all VHRN members on request,including requests from other sectors.
