To explore pharmaceutical actinobacteria from salty environments in Siberia,8 soil samples were collected from different depths of Gudzhirganskoe saline lake,the Republic of Buryatia,Russia.Totally,635 actinobacterial...To explore pharmaceutical actinobacteria from salty environments in Siberia,8 soil samples were collected from different depths of Gudzhirganskoe saline lake,the Republic of Buryatia,Russia.Totally,635 actinobacterial strains affiliated with 21 genera in 12 families of 7 orders were obtained by culture-dependent approaches.The predominant genus was Streptomyces(74.5%),followed by Microbacterium(4.9%),Agromyces(3.5%),Nocardiopsis(3.0%),and Kitasatospora(2.5%).Seven strains showed relatively low 16 S rRNA similarities(<98.65%)with validly described species.Seventy-seven strains were selected as representatives to evaluate their antimicrobial activities and underlying mechanisms by paper-disk diffusion method and a double fluorescent protein reporter system(pDualrep 2),respectively.Streptomyces sp.S 6 b 3-1 was highlighted due to its strong inhibitory activities against Gram-positive bacteria and underlying antibacterial mechanism by inducing the SOS response in the reporter system.LG-1,(R)-6-[1-(benzoyloxy)ethyl]-phenazine-1-carboxylic acid,an analogue of saphenamycin was finally isolated from the culture broth of Streptomyces strain.This study preliminarily explored the actinobacterial diversity of soil samples collected in the highly alkaline and saline lake Gudzhirganskoe by culture-dependent approach and their capacity to produce antibiotics for the first time,which revealed that it deserves to make more efforts to discover new actinobacterial species and potential new antibiotics from the saline lake in Eastern Siberia.展开更多
A new epipolythiodioxopiperazine （ETP）, asperterzine （1）, along with two known analogs, bisdethiobis （methylthio）-acetylaranotin （2） and bisdethiobis（methylthio）-acetylapoaranotin （3）, was isolated from th...A new epipolythiodioxopiperazine （ETP）, asperterzine （1）, along with two known analogs, bisdethiobis （methylthio）-acetylaranotin （2） and bisdethiobis（methylthio）-acetylapoaranotin （3）, was isolated from the plant endophytic fungus Aspergillus terreus PR-P-2. The structure elucidation of 1 was accomplished by a combination of spectral methods and electronic circular dichroism （ECD） spectrum. Asperterzine （1） was a symmetric aromatized ETP found as a natural product for the first time. Compounds 2 and 3 showed strong cytotoxicity against HL-60 cell line. The putative biosynthetic pathway of 1 was also detailed in the text.展开更多
基金Supported by the CAMS Innovation Fund for Medical Sciences(No.CIFMS 2021-I 2 M-1-028)the Beijing Natural Science Foundation(No.7222256)+5 种基金the National Natural Science Foundation of China(No.82011530051)the Kunlun Talented People,High-end Innovation and Entrepreneurship talents Plan,Qinghai Province,China,for Chenghang SUN,the Talent Training Project of Basic Research Fund of Heilongjiang Department of Education Item to Chunmei XUE(No.2020-KYYWF-0238)the Sino-Hungarian Bilateral S&T Cooperation Projects from 2019 to 2020(Chinese Project:8-11)partly supported by the Russian Foundation for Basic Research(Nos.NCFS-RFBR 20-54-53014,18-44-030021 r_а)the Russian Science Foundation(No.20-74-10031)partially within the framework of the State Assignment(No.121030100229-1)for Institute of General and Experimental Biology,Siberian Branch,Russian Academy of Sciences。
文摘To explore pharmaceutical actinobacteria from salty environments in Siberia,8 soil samples were collected from different depths of Gudzhirganskoe saline lake,the Republic of Buryatia,Russia.Totally,635 actinobacterial strains affiliated with 21 genera in 12 families of 7 orders were obtained by culture-dependent approaches.The predominant genus was Streptomyces(74.5%),followed by Microbacterium(4.9%),Agromyces(3.5%),Nocardiopsis(3.0%),and Kitasatospora(2.5%).Seven strains showed relatively low 16 S rRNA similarities(<98.65%)with validly described species.Seventy-seven strains were selected as representatives to evaluate their antimicrobial activities and underlying mechanisms by paper-disk diffusion method and a double fluorescent protein reporter system(pDualrep 2),respectively.Streptomyces sp.S 6 b 3-1 was highlighted due to its strong inhibitory activities against Gram-positive bacteria and underlying antibacterial mechanism by inducing the SOS response in the reporter system.LG-1,(R)-6-[1-(benzoyloxy)ethyl]-phenazine-1-carboxylic acid,an analogue of saphenamycin was finally isolated from the culture broth of Streptomyces strain.This study preliminarily explored the actinobacterial diversity of soil samples collected in the highly alkaline and saline lake Gudzhirganskoe by culture-dependent approach and their capacity to produce antibiotics for the first time,which revealed that it deserves to make more efforts to discover new actinobacterial species and potential new antibiotics from the saline lake in Eastern Siberia.
基金supported by the Fund of Natural Science of Liaoning Province(No. 2015020730)supported by Program for Innovative Research Team of the Ministry of EducationProgram for Liaoning Innovative Research Team in University
文摘A new epipolythiodioxopiperazine （ETP）, asperterzine （1）, along with two known analogs, bisdethiobis （methylthio）-acetylaranotin （2） and bisdethiobis（methylthio）-acetylapoaranotin （3）, was isolated from the plant endophytic fungus Aspergillus terreus PR-P-2. The structure elucidation of 1 was accomplished by a combination of spectral methods and electronic circular dichroism （ECD） spectrum. Asperterzine （1） was a symmetric aromatized ETP found as a natural product for the first time. Compounds 2 and 3 showed strong cytotoxicity against HL-60 cell line. The putative biosynthetic pathway of 1 was also detailed in the text.
