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Development of oligonucleotide probes for FISH karyotyping in Haynaldia villosa,a wild relative of common wheat 被引量:4
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作者 Jia Lei Jiawen Zhou +7 位作者 Haojie Sun Wentao Wan Jin Xiao chunxia yuan Miroslava Karafiátová Jaroslav Dolezel Haiyan Wang Xiue Wang 《The Crop Journal》 SCIE CAS CSCD 2020年第4期676-681,共6页
Haynaldia villosa is a wild relative of wheat and a valuable gene resource for wheat improvement.Owing to the limited number of probes available for fluorescence in situ hybridization(FISH),the resolution at which the... Haynaldia villosa is a wild relative of wheat and a valuable gene resource for wheat improvement.Owing to the limited number of probes available for fluorescence in situ hybridization(FISH),the resolution at which the karyotype of H.villosa can be characterized is poor,hampering accurate characterization of small segmental alien introgressions.We designed ten oligonucleotide probes using tandem repeats in DNA sequences derived from the short arm of H.villosa chromosome 6 V(6 VS).FISH with seven of them resulted in clear signals on H.villosa chromosomes.Using these,we constructed FISH karyotypes for H.villosa using oligo-6 VS-1 and oligo-6 VS-35 oligonucleotides and characterized the distribution of the two probes in five different H.villosa accessions.The new FISH probes can efficiently characterize H.villosa introgressions into wheat. 展开更多
关键词 Chromosome identification Haynaldia villosa Oligonucleotide probes Tandem DNA repeats
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A chromosome-scale genome assembly of Dasypyrum villosum provides insights into its application as a broad-spectrum disease resistance resource for wheat improvement 被引量:2
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作者 Xu Zhang Haiyan Wang +13 位作者 Haojie Sun Yingbo Li Yilong Feng Chengzhi Jiao Mengli Li Xinying Song Tong Wang Zongkuan Wang chunxia yuan Li Sun Ruju Lu Wenli Zhang Jin Xiao Xiue Wang 《Molecular Plant》 SCIE CAS CSCD 2023年第2期432-451,共20页
Dasypyrum villosum is one of the most valuable gene resources in wheat improvement,especially for disease resistance.The mining of favorable genes from D.villosum is frustrated by the lack of a whole genome sequence.I... Dasypyrum villosum is one of the most valuable gene resources in wheat improvement,especially for disease resistance.The mining of favorable genes from D.villosum is frustrated by the lack of a whole genome sequence.In this study,we generated a doubled-haploid line,91C43^(DH),using microspore culture and obtained a 4.05-GB high-quality,chromosome-scale genome assembly for D.villosum.The assembly contains39727 high-confidence genes,and 85.31% of the sequences are repetitive.Two reciprocal translocation events were detected,and 7VS-4VL is a unique translocation in D.villosum.The prolamin seed storage protein-coding genes were found to be duplicated;in particular,the genes encoding low-molecular-weight glutenin at the Glu-V3 locus were significantly expanded.RNA sequencing(RNA-seq)analysis indicated that,after Blumeria graminearum f.sp tritici(Bgt)inoculation,there were more upregulated genes involved in the pattern-triggered immunity and effector-triggered immunity defense pathways in D.villosum than in Triticum urartu.MNase hypersensitive sequencing(MH-seq)identified two Bgt-inducible MH sites(MHSs),one in the promoter and one in the 3'terminal region of the powdery mildew resistance(Pm)gene NLR1-V.Each site had two subpeaks and they were termed MHS1(MHS1.1/1.2)and MHS2(MHS2.1/2.2).Bgt-inducible MHS2.2 was uniquely present in D.villosum,and MHS1.1 was more inducible in D.villosum than in wheat,suggesting that MHSs may be critical for regulation of NLR1-V expression and plant defense.In summary,this study provides a valuable genome resource for functional genomics studies and wheat-D.villosum introgression breeding.The identified regulatory mechanisms may also be exploited to develop new strategies for enhancing Pm resistance by optimizing gene expression in wheat. 展开更多
关键词 D.villosum genome crop wild relative genome evolution disease resistance RNA-seq MNase hy-persensitivityassay
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Construction and application of oligo-based FISH karyotype of Haynaldia villosa 被引量:5
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作者 Haojie Sun Jingjing Song +7 位作者 Jia Lei Xinying Song Keli Dai Jin Xiao chunxia yuan Shengmin An Haiyan Wang Xiue Wang 《Journal of Genetics and Genomics》 SCIE CAS CSCD 2018年第8期463-466,共4页
Haynaldia villosa (L.) Schur (syn. Dasypyrurn villosum (L.) Can- dargy) (2n - 14, genome VV), a wild relative of wheat, is an impor- tant gene pool for improving wheat quality and disease resistance. Several g... Haynaldia villosa (L.) Schur (syn. Dasypyrurn villosum (L.) Can- dargy) (2n - 14, genome VV), a wild relative of wheat, is an impor- tant gene pool for improving wheat quality and disease resistance. Several genes found in H. villosa have been transferred into wheat to improve wheat resistance by the development of alien transloca- tion lines. The seed storage protein loci on chromosome 1V contribute to grain quality (Zhang et al., 2014). 展开更多
关键词 VP Construction and application of oligo-based FISH karyotype of Haynaldia villosa GAA
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A natural variation of an SVP MADS-box transcription factor in Triticum petropavlovskyi leads to its ectopic expression and contributes to elongated glume 被引量:3
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作者 Jin Xiao Yan Chen +13 位作者 Yifan Lu Zhipeng Liu Dongmei Si Tao Xu Li Sun Zongkuan Wang chunxia yuan Haojie Sun Xu Zhang Mingxing Wen Luyang Wei Wenli Zhang Haiyan Wang Xiue Wang 《Molecular Plant》 SCIE CAS CSCD 2021年第9期1408-1411,共4页
Dear Editor,Triticum petropavlovskyi,commonly known as Xinjiang rice wheat,is a unique hexaploidy wheat grown in the Xinjiang area.T.petropavlovskyi is marked by its elongated glume(Eg)trait,which is controlled by gen... Dear Editor,Triticum petropavlovskyi,commonly known as Xinjiang rice wheat,is a unique hexaploidy wheat grown in the Xinjiang area.T.petropavlovskyi is marked by its elongated glume(Eg)trait,which is controlled by gene P1pet located on chromosome 7A(Wang et al.,2002;Watanabe and Imamura,2002).The origin of T.petropavlovskyi has been disputed.It is generally accepted that P1pet was introduced from tetraploid polish wheat(T.polonicum)via a hybridization with hexaploid common wheat(T.aestivum)(Akond et al.,2008;Chen et al.,2016). 展开更多
关键词 TRITICUM Xinjiang wheat
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