Haynaldia villosa is a wild relative of wheat and a valuable gene resource for wheat improvement.Owing to the limited number of probes available for fluorescence in situ hybridization(FISH),the resolution at which the...Haynaldia villosa is a wild relative of wheat and a valuable gene resource for wheat improvement.Owing to the limited number of probes available for fluorescence in situ hybridization(FISH),the resolution at which the karyotype of H.villosa can be characterized is poor,hampering accurate characterization of small segmental alien introgressions.We designed ten oligonucleotide probes using tandem repeats in DNA sequences derived from the short arm of H.villosa chromosome 6 V(6 VS).FISH with seven of them resulted in clear signals on H.villosa chromosomes.Using these,we constructed FISH karyotypes for H.villosa using oligo-6 VS-1 and oligo-6 VS-35 oligonucleotides and characterized the distribution of the two probes in five different H.villosa accessions.The new FISH probes can efficiently characterize H.villosa introgressions into wheat.展开更多
Dasypyrum villosum is one of the most valuable gene resources in wheat improvement,especially for disease resistance.The mining of favorable genes from D.villosum is frustrated by the lack of a whole genome sequence.I...Dasypyrum villosum is one of the most valuable gene resources in wheat improvement,especially for disease resistance.The mining of favorable genes from D.villosum is frustrated by the lack of a whole genome sequence.In this study,we generated a doubled-haploid line,91C43^(DH),using microspore culture and obtained a 4.05-GB high-quality,chromosome-scale genome assembly for D.villosum.The assembly contains39727 high-confidence genes,and 85.31% of the sequences are repetitive.Two reciprocal translocation events were detected,and 7VS-4VL is a unique translocation in D.villosum.The prolamin seed storage protein-coding genes were found to be duplicated;in particular,the genes encoding low-molecular-weight glutenin at the Glu-V3 locus were significantly expanded.RNA sequencing(RNA-seq)analysis indicated that,after Blumeria graminearum f.sp tritici(Bgt)inoculation,there were more upregulated genes involved in the pattern-triggered immunity and effector-triggered immunity defense pathways in D.villosum than in Triticum urartu.MNase hypersensitive sequencing(MH-seq)identified two Bgt-inducible MH sites(MHSs),one in the promoter and one in the 3'terminal region of the powdery mildew resistance(Pm)gene NLR1-V.Each site had two subpeaks and they were termed MHS1(MHS1.1/1.2)and MHS2(MHS2.1/2.2).Bgt-inducible MHS2.2 was uniquely present in D.villosum,and MHS1.1 was more inducible in D.villosum than in wheat,suggesting that MHSs may be critical for regulation of NLR1-V expression and plant defense.In summary,this study provides a valuable genome resource for functional genomics studies and wheat-D.villosum introgression breeding.The identified regulatory mechanisms may also be exploited to develop new strategies for enhancing Pm resistance by optimizing gene expression in wheat.展开更多
Haynaldia villosa (L.) Schur (syn. Dasypyrurn villosum (L.) Can- dargy) (2n - 14, genome VV), a wild relative of wheat, is an impor- tant gene pool for improving wheat quality and disease resistance. Several g...Haynaldia villosa (L.) Schur (syn. Dasypyrurn villosum (L.) Can- dargy) (2n - 14, genome VV), a wild relative of wheat, is an impor- tant gene pool for improving wheat quality and disease resistance. Several genes found in H. villosa have been transferred into wheat to improve wheat resistance by the development of alien transloca- tion lines. The seed storage protein loci on chromosome 1V contribute to grain quality (Zhang et al., 2014).展开更多
Dear Editor,Triticum petropavlovskyi,commonly known as Xinjiang rice wheat,is a unique hexaploidy wheat grown in the Xinjiang area.T.petropavlovskyi is marked by its elongated glume(Eg)trait,which is controlled by gen...Dear Editor,Triticum petropavlovskyi,commonly known as Xinjiang rice wheat,is a unique hexaploidy wheat grown in the Xinjiang area.T.petropavlovskyi is marked by its elongated glume(Eg)trait,which is controlled by gene P1pet located on chromosome 7A(Wang et al.,2002;Watanabe and Imamura,2002).The origin of T.petropavlovskyi has been disputed.It is generally accepted that P1pet was introduced from tetraploid polish wheat(T.polonicum)via a hybridization with hexaploid common wheat(T.aestivum)(Akond et al.,2008;Chen et al.,2016).展开更多
基金supported by the National Key Research and Development Program of China(2016YFD0102001)the National Natural Science Foundation of China(31571653,31771782,31201204,31501305)+3 种基金International Cooperation and Exchange Programme of the National Natural Science Foundation of China(31661143005)Introducing the Technique to Exploring the Genetic Germplasm Based on the Chromosome Sorting and Sequencing(2015-Z41)the Special Fund of Jiangsu Province for the Transformation of Scientific and Technological Achievements(BA2017138)supported by European Regional Development Fund Project“Plants as a Tool for Sustainable Global Development”(CZ.02.1.01/0.0/0.0/16_019/0000827)。
文摘Haynaldia villosa is a wild relative of wheat and a valuable gene resource for wheat improvement.Owing to the limited number of probes available for fluorescence in situ hybridization(FISH),the resolution at which the karyotype of H.villosa can be characterized is poor,hampering accurate characterization of small segmental alien introgressions.We designed ten oligonucleotide probes using tandem repeats in DNA sequences derived from the short arm of H.villosa chromosome 6 V(6 VS).FISH with seven of them resulted in clear signals on H.villosa chromosomes.Using these,we constructed FISH karyotypes for H.villosa using oligo-6 VS-1 and oligo-6 VS-35 oligonucleotides and characterized the distribution of the two probes in five different H.villosa accessions.The new FISH probes can efficiently characterize H.villosa introgressions into wheat.
基金financially supported by the National Key Agriculture Projects(NK2022060101)National Key Research and Development Program(2022YFF1002900,2020YFE0202900)+3 种基金the Fundamental Research Funds for the Central University(XUEKEN2022012)Jiangsu Provincial Key Research and Development Program(BE2021375,BE2022346)Seed Industry Revitalization Project of Jiangsu Province(JBGS2021006,2021013,2021047)Joint Research of Improved Wheat Variety of Anhui,and Jiangsu Agricultural Technology System(JATS)(JATS[2021]463,JATS[2022]464).
文摘Dasypyrum villosum is one of the most valuable gene resources in wheat improvement,especially for disease resistance.The mining of favorable genes from D.villosum is frustrated by the lack of a whole genome sequence.In this study,we generated a doubled-haploid line,91C43^(DH),using microspore culture and obtained a 4.05-GB high-quality,chromosome-scale genome assembly for D.villosum.The assembly contains39727 high-confidence genes,and 85.31% of the sequences are repetitive.Two reciprocal translocation events were detected,and 7VS-4VL is a unique translocation in D.villosum.The prolamin seed storage protein-coding genes were found to be duplicated;in particular,the genes encoding low-molecular-weight glutenin at the Glu-V3 locus were significantly expanded.RNA sequencing(RNA-seq)analysis indicated that,after Blumeria graminearum f.sp tritici(Bgt)inoculation,there were more upregulated genes involved in the pattern-triggered immunity and effector-triggered immunity defense pathways in D.villosum than in Triticum urartu.MNase hypersensitive sequencing(MH-seq)identified two Bgt-inducible MH sites(MHSs),one in the promoter and one in the 3'terminal region of the powdery mildew resistance(Pm)gene NLR1-V.Each site had two subpeaks and they were termed MHS1(MHS1.1/1.2)and MHS2(MHS2.1/2.2).Bgt-inducible MHS2.2 was uniquely present in D.villosum,and MHS1.1 was more inducible in D.villosum than in wheat,suggesting that MHSs may be critical for regulation of NLR1-V expression and plant defense.In summary,this study provides a valuable genome resource for functional genomics studies and wheat-D.villosum introgression breeding.The identified regulatory mechanisms may also be exploited to develop new strategies for enhancing Pm resistance by optimizing gene expression in wheat.
基金supported by the grants from the National Key Research and Development Program (2016YFD0102001)the National Natural Science Foundation of China (Nos.31571653,31771782,31201204,and 31501305)+6 种基金the International Cooperation and Exchange of the National Natural Science Foundation of China (No.31661143005)the ‘948’ Project of Ministry of Agriculture (2015-Z41)the Fundamental Research Funds for the Central Universities (KYZ201403 and KJ2013003)the Technology Support Program of Jiangsu Province (BE2015352-2)the special fund of Jiangsu Province for the transformation of scientific and technological achievements (BA2017138)the Program of Introducing Talents of Discipline to Universities (B08025)the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
文摘Haynaldia villosa (L.) Schur (syn. Dasypyrurn villosum (L.) Can- dargy) (2n - 14, genome VV), a wild relative of wheat, is an impor- tant gene pool for improving wheat quality and disease resistance. Several genes found in H. villosa have been transferred into wheat to improve wheat resistance by the development of alien transloca- tion lines. The seed storage protein loci on chromosome 1V contribute to grain quality (Zhang et al., 2014).
基金This research was supported by the funds from the National Key Research and Developme nt Program,China(grant nos.2016YFD0100302 and 2020YFE0202900)the National Natural Science Foundation of China,China(grant no.91935304)+2 种基金the International Coop-eration and Exchange of the National Natural Science Foundation of China,China(grant no.31661143005)the Jiangsu Agricultural Technology System,China(JATS)(no.2020411)the SAAS Program for Excellent Research Team,China,and the Key Research and Development Major Project of Ningxia Hui Autonomous Region,China(no.2019BBF02022-04).
文摘Dear Editor,Triticum petropavlovskyi,commonly known as Xinjiang rice wheat,is a unique hexaploidy wheat grown in the Xinjiang area.T.petropavlovskyi is marked by its elongated glume(Eg)trait,which is controlled by gene P1pet located on chromosome 7A(Wang et al.,2002;Watanabe and Imamura,2002).The origin of T.petropavlovskyi has been disputed.It is generally accepted that P1pet was introduced from tetraploid polish wheat(T.polonicum)via a hybridization with hexaploid common wheat(T.aestivum)(Akond et al.,2008;Chen et al.,2016).