Alpha linolenic acid(ALA)is an essential polyunsaturated fatty acids that can improve human health.Rapeseed is the second largest oil crop in the world but the ALA content in its seed fatty acids is only about 10%.Two...Alpha linolenic acid(ALA)is an essential polyunsaturated fatty acids that can improve human health.Rapeseed is the second largest oil crop in the world but the ALA content in its seed fatty acids is only about 10%.Two rapeseed germplasms YH25005 and R8Q10 with high content(up to 21%)ALA were developed by intervarietal crossing.They were used as the maternal parent(P_(1))when crossed with a low ALA parent SW(P2)to produce the seeds of the F1 hybrid,F2,and backcrosses to P1(BCP1)and P2(BCP2).A multigeneration joint segregation analysis was conducted to determine major gene t polygene effects of the content of three major unsaturated fatty acids including oleic(OA),linoleic(LA)and ALA.The results showed that,although some genes favorable to ALA accumulation were not allelic in R8Q10 and YH25005,all the inheritances of OA,LA and ALA in YH25005×SW and R8Q10×SW followed a genetic model of‘two pairs of additive major genes t additive-dominant polygenes’.It is suggested that the contents of OA,LA and ALA are closely related and the major genes in the parent SW containing loss-of-function mutations in FAD2 and FAD3 loci had strong effect to reduce ALA and elevate OA.However,total genetic effect of the polygenes was greater than that of the two major genes,especial on ALA content.It indicated that it is necessary to employ a larger F2 population to find the plant that accumulate enough minor-effect polygenes for high ALA content.The results are useful for high ALA rapeseed breeding and future work of gene mapping.展开更多
Dysregulated crosstalk between different signaling pathways contributes to tumor development,including resistance to cancer therapy.In the present study,we found that the mitogen-activated extracellular signal-regulat...Dysregulated crosstalk between different signaling pathways contributes to tumor development,including resistance to cancer therapy.In the present study,we found that the mitogen-activated extracellular signal-regulated kinase(MEK)inhibitor trametinib failed to suppress the proliferation of PANC-1 and MGC803 cells by activating the Janus kinase 2(JAK2)/signal transducer and activator of transcription 3(STAT3)signaling pathway,while the JAK2 inhibitor fedratinib failed to inhibit the growth of the PANC-1 cells upon stimulation of extracellular signal-regulated kinase(ERK)signaling.In particular,the most prominent enhancement of the anti-proliferative effect resulted from the concurrent blockage of the JAK2/STAT3 and ERK signaling pathways.Furthermore,the combination of the two inhibitors resulted in a reduced tumor burden in mice.Our evidence suggests novel crosstalk between JAK2/STAT3 and ERK signaling in gastric cancer(GC)and pancreatic ductal adenocarcinoma(PDAC)cells and provides a therapeutic strategy to overcome potential resistance in gastrointestinal cancer.展开更多
A recent article by Robiou du Pont and Meinshausen [1](hereafter RM2018) declared that when taken as benchmark by other countries, the Nationally Determined Contributions (NDCs) of India, the EU, the USA and China lea...A recent article by Robiou du Pont and Meinshausen [1](hereafter RM2018) declared that when taken as benchmark by other countries, the Nationally Determined Contributions (NDCs) of India, the EU, the USA and China lead to 2.6, 3.2, 4 and over 5.1 °C warmings. RM2018 was quickly referred in media that China's NDC is "one of the worst to contribute to the global effort to reach the targets under Paris Agreement”.展开更多
基金supported by the project of Key R&D plans of Shaanxi Province(2021NY-074)Yangling Seed Industry Innovation Plan.
文摘Alpha linolenic acid(ALA)is an essential polyunsaturated fatty acids that can improve human health.Rapeseed is the second largest oil crop in the world but the ALA content in its seed fatty acids is only about 10%.Two rapeseed germplasms YH25005 and R8Q10 with high content(up to 21%)ALA were developed by intervarietal crossing.They were used as the maternal parent(P_(1))when crossed with a low ALA parent SW(P2)to produce the seeds of the F1 hybrid,F2,and backcrosses to P1(BCP1)and P2(BCP2).A multigeneration joint segregation analysis was conducted to determine major gene t polygene effects of the content of three major unsaturated fatty acids including oleic(OA),linoleic(LA)and ALA.The results showed that,although some genes favorable to ALA accumulation were not allelic in R8Q10 and YH25005,all the inheritances of OA,LA and ALA in YH25005×SW and R8Q10×SW followed a genetic model of‘two pairs of additive major genes t additive-dominant polygenes’.It is suggested that the contents of OA,LA and ALA are closely related and the major genes in the parent SW containing loss-of-function mutations in FAD2 and FAD3 loci had strong effect to reduce ALA and elevate OA.However,total genetic effect of the polygenes was greater than that of the two major genes,especial on ALA content.It indicated that it is necessary to employ a larger F2 population to find the plant that accumulate enough minor-effect polygenes for high ALA content.The results are useful for high ALA rapeseed breeding and future work of gene mapping.
基金the Zhejiang Provincial Natural Science Foundation of China(Nos.LQ18H280005 and LY21H030002)the National Natural Science Foundation of China(Nos.81770535,81600595,81503297,81603340,81773945,and 81803775)+1 种基金the Medical Health Science and Technology Project of Zhejiang Provincial Health Commission(Nos.2019RC228,2019RC229,and 2019RC113)the Open Foundation from Chinese and Western Integrative Medicine in the Most Important Subjects of Zhejiang(No.ZXYJH2018002),China。
文摘Dysregulated crosstalk between different signaling pathways contributes to tumor development,including resistance to cancer therapy.In the present study,we found that the mitogen-activated extracellular signal-regulated kinase(MEK)inhibitor trametinib failed to suppress the proliferation of PANC-1 and MGC803 cells by activating the Janus kinase 2(JAK2)/signal transducer and activator of transcription 3(STAT3)signaling pathway,while the JAK2 inhibitor fedratinib failed to inhibit the growth of the PANC-1 cells upon stimulation of extracellular signal-regulated kinase(ERK)signaling.In particular,the most prominent enhancement of the anti-proliferative effect resulted from the concurrent blockage of the JAK2/STAT3 and ERK signaling pathways.Furthermore,the combination of the two inhibitors resulted in a reduced tumor burden in mice.Our evidence suggests novel crosstalk between JAK2/STAT3 and ERK signaling in gastric cancer(GC)and pancreatic ductal adenocarcinoma(PDAC)cells and provides a therapeutic strategy to overcome potential resistance in gastrointestinal cancer.
基金supported by the National Key Research and Development Program of China(2017YFA0605302)
文摘A recent article by Robiou du Pont and Meinshausen [1](hereafter RM2018) declared that when taken as benchmark by other countries, the Nationally Determined Contributions (NDCs) of India, the EU, the USA and China lead to 2.6, 3.2, 4 and over 5.1 °C warmings. RM2018 was quickly referred in media that China's NDC is "one of the worst to contribute to the global effort to reach the targets under Paris Agreement”.
