Mg^(2+),Al^(3+)和Fe^(3+)对氟磷灰石表面性质的影响

矿物表面性质是影响其分散聚集和浮选行为的主要因素,通过溶液化学分析计算、浊度、接触角及Zeta-电位测试分析,研究了矿浆中,Mg^(2+),Al^(3+),Fe^(3+)等金属离子对氟磷灰石(FAP)表面性质的影响。结果表明:金属离子在溶液中的存在形式随pH值的变化而改变,FAP是亲水性矿物,添加Fe^(3+)对FAP的润湿性影响受pH的影响较大,在酸性环境中,Fe^(3+)对FAP的亲水起促进作用,在中性及碱性环境下,Fe^(3+)能较好地抑制FAP的亲水。Mg^(2+)和Al^(3+)受pH的影响较小,在中性环境下Mg^(2+),Al^(3+)离子均抑制FAP的润湿性。在pH=3~11范围内,Mg^(2+)主要以+2价的离子存在,压缩FAP表面双电层;Al^(3+)和Fe^(3+)主要是通过水解的作用生成氢氧化物沉淀覆盖在FAP表面,并且Mg^(2+),Al^(3+),Fe^(3+)均会破坏FAP的分散性,促使FAP发生聚沉现象。

StRAP2.3, an ERF-Ⅶ transcription factor, directly activates StInvInh2 to enhance cold-induced sweetening resistance in potato

Potato invertase inhibitor(StInvInh2)positively regulates cold-induced sweetening(CIS)resistance by inhibiting the activity of vacuolar invertase.The distinct expression patterns of StInvInh2 have been thoroughly characterized in different potato genotypes,but the related CIS ability has not been characterized.The understanding of the regulatory mechanisms that control StInvInh2 transcription is unclear.In this study,we identified an ERF‐Ⅶ transcription factor,StRAP2.3,that directly regulates StInvInh2 to positively modulate CIS resistance.Acting as a nuclear-localized transcriptional activator,StRAP2.3 directly binds the ACCGAC cis-element in the promoter region of StInvInh2,enabling promoter activity.Overexpression of StRAP2.3 in CIS-sensitive potato tubers induced StInvInh2 mRNA abundance and increased CIS resistance.In contrast,silencing StRAP2.3 in CIS-resistant potato tubers repressed the expression of StInvInh2 and decreased CIS resistance.We conclude that cold-responsive StInvInh2 is due to the binding of StRAP2.3 to the ACCGAC cis-element in the promoter region of StInvInh2.Overall,these findings indicate that StRAP2.3 directly regulates StInvInh2 to positively modulate CIS resistance,which may provide a strategy to improve the processing quality of potatoes.

BMI对早卵泡期长效长方案IVF/ICSI患者新鲜周期胚胎移植妊娠结局的影响

目的探究体质指数(BMI)对于进行早卵泡期长效长方案体外受精或卵母细胞胞质内单精子注射法(IVF/ICSI)治疗患者的治疗过程以及新鲜周期胚胎移植妊娠结局的影响。方法回顾性分析2018年1月1日至2020年7月1日于南京医科大学第一附属医院首次行早卵泡期长效长方案的IVF/ICSI治疗患者的临床资料。按照患者的BMI分为低BMI组(BMI<18.5 kg/m^(2),141例)、正常BMI组(18.5≤BMI<24.0 kg/m^(2),1663例)、超重肥胖组(BMI≥24.0 kg/m^(2),453例),分析各组患者的诱导排卵指标有无差异;再从3组中筛选出进行新鲜胚胎移植的患者分别命名为低BMI新鲜组(102例)、正常BMI新鲜组(1266例)、超重肥胖新鲜组(373例),比较各组的诱导排卵情况和妊娠结局。结果本研究共纳入2257例患者,低BMI组、正常BMI组及超重肥胖组患者的促性腺激素(Gn)启动剂量、Gn总天数、Gn总剂量均有显著差异(P均<0.01),超重肥胖组Gn启动剂量[(162±40)U]、Gn总剂量[(2159±668)U]均最多、Gn总天数[(12.3±2.5)d]最长。超重肥胖组hCG日雌二醇及孕酮水平[分别为(7474±4852)pmol/L、(3.4±1.9)nmol/L]均显著低于低BMI组及正常BMI组(P均<0.01),获卵数[(8.4±4.1)个]较正常BMI组减少(P<0.05)。超重肥胖组患者的正常受精数、可移植胚胎数、高评分卵裂期胚胎数均较其他2组略减少,但差异均无统计学意义(P均>0.05)。进行新鲜胚胎移植的1741例患者中,低BMI新鲜组的移植胚胎数[(1.2±0.4)个]较其他2组减少(P<0.05),正常BMI新鲜组患者的生化妊娠率、临床妊娠率、活产率较其他2组增高,但差异均无统计学意义(P均>0.05)。结论在进行早卵泡期长方案lVF/ICSI治疗的患者中,BMI升高可能会影响诱导排卵反应,导致Gn用量增加、Gn诱导排卵的时间延长。虽然不同BMI的各组新鲜周期胚胎移植的妊娠结局尚无统计学差异,但考虑超重、肥胖患者后续妊娠后围产期不良风险增高,故临床工作中对于行早卵泡期长方案治疗的患者仍应重视控制BMI。

Self-powered electrochromic devices with tunable infrared intensity

Triboelectric nanogenerator(TENG) is an efficient way to convert ambient mechanical energy into electricity to power up portable electronics.In this work,a flexible infrared electrochromical device(IR-ECD)with stable performances was assembled with a TENG for building self-powered infrared detector with tunable intensity.As driven by TENG,the electrochromic device could be operated in the mid-IR region due to the reversible electrochromic reactions.An average infrared reflectance contrast of 46% was achieved in 8–14 lm regions and as well a clear thermal image change can be observed.This work indicates that the TENG-driven infrared electrochromical device has potential for use in self-powered camouflage and thermal control.

A novel long-term intravenous combined with local treatment with human amnion-derived mesenchymal stem cells for a multidisciplinary rescued uremic calciphylaxis patient and the underlying mechanism

Calciphylaxis is a rare disease characterized histologically by microvessel calcification and microthrombosis,with high mortality and no proven therapy.Here,we reported a severe uremic calciphylaxis patient with progressive skin ischemia,large areas of painful malodorous ulcers,and mummified legs.Because of the worsening symptoms and signs refractory to conventional therapies,treatment with human amnion-derived mesenchymal stem cells(hAMSCs)was approved.Preclinical release inspections of hAMSCs,efficacy,and safety assessment,including cytokine secretory ability,immunocompetence,tumorigenicity,and genetics analysis in vitro,were introduced.We further performed acute and long-term hAMSC toxicity evaluations in C57BL/6 mice and rats,abnormal immune response tests in C57BL/6 mice,and tumorigenicity tests in neonatal Balbc-nu nude mice.After the preclinical research,the patient was treated with hAMSCs by intravenous and local intramuscular injection and external supernatant application to the ulcers.When followed up to 15 months,the blood-based markers of bone and mineral metabolism improved,with skin soft tissue regeneration and a more favorable profile of peripheral blood mononuclear cells.Skin biopsy after 1-month treatment showed vascular regeneration with mature noncalcified vessels within the dermis,and 20 months later,the re-epithelialization restored the integrity of the damaged site.No infusion or local treatment-related adverse events occurred.Thus,this novel long-term intravenous combined with local treatment with hAMSCs warrants further investigation as a potential regenerative treatment for uremic calciphylaxis due to effects of inhibiting vascular calcification,stimulating angiogenesis and myogenesis,anti-inflammatory and immune modulation,multidifferentiation,re-epithelialization,and restoration of integrity.