Objective: To deeply explore the molecular mechanism of MCLR in the course of MCLR promoting liver tumor. Methods: We applied the two -stage- medium-term theory to set up the animal model, and biochemistry technique t...Objective: To deeply explore the molecular mechanism of MCLR in the course of MCLR promoting liver tumor. Methods: We applied the two -stage- medium-term theory to set up the animal model, and biochemistry technique to evalu- ate the model, and then, used RT-PCR technique and image analysis to study the mRNA expression of the p53 and p16 in the course of promoting tumor. Results: (1) MCLR could enhance the positive reaction ratio of γ-Glutamyltransferase(GGT). (2) MCLR could increase the mRNA expression of p53, and had not effect on the mRNA expression of p53. Conclusion: (1) MC can promote liver tumor strongly. (2) The expression change of p53 may play an important role in the course of MCLR promoting liver tumor.展开更多
基金Supported by the grants from the Natural Science Foundation of Fujian Province (No. C0210011)the Science &Technology Program for the Education Department of Fujian Province (No. JA05256).
文摘Objective: To deeply explore the molecular mechanism of MCLR in the course of MCLR promoting liver tumor. Methods: We applied the two -stage- medium-term theory to set up the animal model, and biochemistry technique to evalu- ate the model, and then, used RT-PCR technique and image analysis to study the mRNA expression of the p53 and p16 in the course of promoting tumor. Results: (1) MCLR could enhance the positive reaction ratio of γ-Glutamyltransferase(GGT). (2) MCLR could increase the mRNA expression of p53, and had not effect on the mRNA expression of p53. Conclusion: (1) MC can promote liver tumor strongly. (2) The expression change of p53 may play an important role in the course of MCLR promoting liver tumor.
