Effect of microRNA-143-3p-mediated CTNND1 on the biological function of lung cancer cells

Lung cancer poses a serious threat to human life with high incidence and miRNA is an important biomarkerin tumors. This study aimed to explore the effect of miR-143-3p on the biological function of lung cancer cells and theunderlying mechanism. Eighty-seven samples of lung cancer tissues and 81 samples of tumor-adjacent tissues from patients undergoing radical lung cancer surgery in our hospital were collected. The lung cancer cells and lung fibroblastcells (HFL-1) were purchased, and then miR-143-3p-mimics, miR-NC, si-CTNND1, and NC were transfected into A549 and PC-9 cells to establish cell models. MiR-143-3p and CTNND1 expression levels were measured by the qRT-PCR, Bax, Bcl-2, and CTNND1 expression levels by the Western Blot (WB), and cell proliferation, invasion, and apoptosis by the MTT assay, Transwell assay, and flow cytometry. Dual luciferase report assay was used to determinethe relationship between miR-143-3p and CTNND1. In this study, miR-143-3p was lowly expressed in lung cancer and CTNND1 was highly expressed in lung cancer. The overexpression of miR-143-3p inhibited cell proliferation and invasion, promoted cell apoptosis, significantly increased Bax protein expression, and decreased Bcl-2 protein expression. The inhibition of CTNND1 led to opposite biological characteristic in cells. The dual luciferase reporter assay demonstrated that miR-143-3p was a target region of CTNND1. Such results suggest that miR-143-3p can inhibitthe proliferation and invasion of lung cancer cells by regulating the expression of CTNND1 and promote the apoptosisof lung cancer cells, sott is expected to be a potential target for lung cancer.

Extraction and Drying Technique of Bawei Shangke Huoxue Tablets

[Objectives]To optimize the water extraction process of Bawei Shangke Huoxue tablets and determine the best drying method of the extract.[Methods]The orthogonal test method was used to select the optimal water extraction process,with the content of total flavonoids and the yield of dry extract as the test indicators,and the soaking time,the amount of water added,the extraction time and the extraction times as the main test factors.The water content of spray powder was taken as the evaluation indicator,the inlet air temperature,the density of liquid phase and the outlet air temperature were selected as the factors,and the spray drying process of water-extracted concentrated liquid was optimized by orthogonal test.[Results]The optimal water extraction process was no immersion,water decoction and extraction twice,adding 10 times of water each time,extraction for 1h,in which the amount of water added was the main factor.The liquid density of spray dried extract powder is 1.05-1.08(60℃),the inlet air temperature is 180℃,the outlet air temperature is 80℃is the most suitable,and the liquid density is the main factor.[Conclusions]The extraction and drying technology of Bawei Huangke Huoxue tablet is feasible,scientific and reasonable,and can be used for industrial production.