Bone marrow mesenchymal stem cells can differentiate into neurons and astrocytes after trans- plantation in the spinal cord of rats with ischemia/reperfusion injury. Although bone marrow mesenchymal stem cells are kno...Bone marrow mesenchymal stem cells can differentiate into neurons and astrocytes after trans- plantation in the spinal cord of rats with ischemia/reperfusion injury. Although bone marrow mesenchymal stem cells are known to protect against spinal cord ischemia/reperfusion injury through anti-apoptotic effects, the precise mechanisms remain unclear. In the present study, bone marrow mesenchymal stem cells were cultured and proliferated, then transplanted into rats with ischemia/reperfusion injury via retro-orbital injection. Immunohistochemistry and immunofluorescence with subsequent quantification revealed that the expression of the axonal regeneration marker, growth associated protein-43, and the neuronal marker, microtubule-as- sociated protein 2, significantly increased in rats with bone marrow mesenchymal stem cell transplantation compared with those in rats with spinal cord ischemia/reperfusion injury. Fur- thermore, the expression of the autophagy marker, microtubule-associated protein light chain 3B, and Beclin 1, was significantly reduced in rats with the bone marrow mesenchymal stem cell transplantation compared with those in rats with spinal cord ischemia/reperfusion injury. Western blot analysis showed that the expression of growth associated protein-43 and neuro- filament-H increased but light chain 3B and Beclin 1 decreased in rats with the bone marrow mesenchymal stem cell transplantation. Our results therefore suggest that bone marrow mes- enchymal stem cell transplantation promotes neurite growth and regeneration and prevents autophagy. These responses may likely be mechanisms underlying the protective effect of bone marrow mesenchymal stem cells against spinal cord ischemia/reperfusion injury.展开更多
Chitosan, collagen I and gelatin were mixed in appropriate quantities to develop a new nerve repair material, with good arrangement and structure, as well as even aperture size. The composite material was sterilized b...Chitosan, collagen I and gelatin were mixed in appropriate quantities to develop a new nerve repair material, with good arrangement and structure, as well as even aperture size. The composite material was sterilized by 60Co irradiation for 24 hours prior to implantation in the right thigh of rats following sciatic nerve damage. Results showed that the material was nontoxic to the kidneys and the liver, and did not induce an inflammatory response in the muscles. The composite material enhanced the recovery of sciatic nerve damage in rats. These experimental findings indicate that the composite material offers good biocompatibility and has a positive effect on injured nerve rehabilitation.展开更多
In recent years,the Ministry of Agriculture has increased input in agricultural scientific research projects.As a result,the procurement amount and quantity of special materials are increasing gradually,and the manage...In recent years,the Ministry of Agriculture has increased input in agricultural scientific research projects.As a result,the procurement amount and quantity of special materials are increasing gradually,and the management of special materials becomes more standardized.In order to do well in management of special materials,the authors made exploration and thinking about further strengthening management of special materials in combination with actual conditions,including establishing regulations and systems,enhancing procurement process,strengthening requisition and safekeeping,and reinforcing general ledger management of special materials.展开更多
Osteosarcoma(OS)is the most common primary malignant bone tumor that more commonly occurs in children and adolescents.The most commonly used treatment for OS is surgery combined with chemotherapy,but the treatment out...Osteosarcoma(OS)is the most common primary malignant bone tumor that more commonly occurs in children and adolescents.The most commonly used treatment for OS is surgery combined with chemotherapy,but the treatment outcomes are typically unsatisfactory.High rates of metastasis and post-treatment recurrence rates are major challenges in the treatment of OS.This underlines the need for studying the in-depth characterization of the pathogenetic mechanisms of OS and development of more effective therapeutic modalities.Previous studies have demonstrated the important role of the bone microenvironment and the regulation of signaling pathways in the occurrence and development of OS.In this review,we discussed the available evidence pertaining to the mechanisms of OS development and identified therapeutic targets for OS.We also summarized the available treatment modalities for OS and identified future priorities for therapeutics research.展开更多
基金supported by the National Natural Science Foundation of China,No.30972153the Science and Technology Development Program of Jilin Provincial Science and Technology Department in China,No.200905183the Scientific Research Foundation of Jilin Department of Health of China,No.2008Z041
文摘Bone marrow mesenchymal stem cells can differentiate into neurons and astrocytes after trans- plantation in the spinal cord of rats with ischemia/reperfusion injury. Although bone marrow mesenchymal stem cells are known to protect against spinal cord ischemia/reperfusion injury through anti-apoptotic effects, the precise mechanisms remain unclear. In the present study, bone marrow mesenchymal stem cells were cultured and proliferated, then transplanted into rats with ischemia/reperfusion injury via retro-orbital injection. Immunohistochemistry and immunofluorescence with subsequent quantification revealed that the expression of the axonal regeneration marker, growth associated protein-43, and the neuronal marker, microtubule-as- sociated protein 2, significantly increased in rats with bone marrow mesenchymal stem cell transplantation compared with those in rats with spinal cord ischemia/reperfusion injury. Fur- thermore, the expression of the autophagy marker, microtubule-associated protein light chain 3B, and Beclin 1, was significantly reduced in rats with the bone marrow mesenchymal stem cell transplantation compared with those in rats with spinal cord ischemia/reperfusion injury. Western blot analysis showed that the expression of growth associated protein-43 and neuro- filament-H increased but light chain 3B and Beclin 1 decreased in rats with the bone marrow mesenchymal stem cell transplantation. Our results therefore suggest that bone marrow mes- enchymal stem cell transplantation promotes neurite growth and regeneration and prevents autophagy. These responses may likely be mechanisms underlying the protective effect of bone marrow mesenchymal stem cells against spinal cord ischemia/reperfusion injury.
基金supported by the Department of Science and Technology of Jilin Province, China, No. 20070417
文摘Chitosan, collagen I and gelatin were mixed in appropriate quantities to develop a new nerve repair material, with good arrangement and structure, as well as even aperture size. The composite material was sterilized by 60Co irradiation for 24 hours prior to implantation in the right thigh of rats following sciatic nerve damage. Results showed that the material was nontoxic to the kidneys and the liver, and did not induce an inflammatory response in the muscles. The composite material enhanced the recovery of sciatic nerve damage in rats. These experimental findings indicate that the composite material offers good biocompatibility and has a positive effect on injured nerve rehabilitation.
文摘In recent years,the Ministry of Agriculture has increased input in agricultural scientific research projects.As a result,the procurement amount and quantity of special materials are increasing gradually,and the management of special materials becomes more standardized.In order to do well in management of special materials,the authors made exploration and thinking about further strengthening management of special materials in combination with actual conditions,including establishing regulations and systems,enhancing procurement process,strengthening requisition and safekeeping,and reinforcing general ledger management of special materials.
基金supported by a grant from the National Natural Science Foundation of China(No.81871814).
文摘Osteosarcoma(OS)is the most common primary malignant bone tumor that more commonly occurs in children and adolescents.The most commonly used treatment for OS is surgery combined with chemotherapy,but the treatment outcomes are typically unsatisfactory.High rates of metastasis and post-treatment recurrence rates are major challenges in the treatment of OS.This underlines the need for studying the in-depth characterization of the pathogenetic mechanisms of OS and development of more effective therapeutic modalities.Previous studies have demonstrated the important role of the bone microenvironment and the regulation of signaling pathways in the occurrence and development of OS.In this review,we discussed the available evidence pertaining to the mechanisms of OS development and identified therapeutic targets for OS.We also summarized the available treatment modalities for OS and identified future priorities for therapeutics research.
