[Objectives]To explore the effect of psoralen combined with paclitaxel on the apoptosis of MCF-7 cells.[Methods]The effects of different concentrations of psoralen,paclitaxel,or the combination of psoralen and paclita...[Objectives]To explore the effect of psoralen combined with paclitaxel on the apoptosis of MCF-7 cells.[Methods]The effects of different concentrations of psoralen,paclitaxel,or the combination of psoralen and paclitaxel on cell viability were detected using CCK-8 assay kit.Cell cycle distribution and apoptosis after 24 h of psoralen(0.16,0.32,0.64 mmol/L),paclitaxel(0.1μmol/L),combined action of psoralen(0.32 mmol/L)and paclitaxel(0.1μmol/L)were detected using flow cytometry.[Results]Lower concentration of psoralen(0.04-0.32 mmol/L)showed no significant inhibitory effect on cells.After combined with paclitaxel,the inhibitory effect on MCF-7 cell proliferation was significantly higher than that of the group treated alone.Compared with the paclitaxel group,the cell apoptosis rate in the drug combination group was significantly increased.Different low concentrations of psoralen can block the cell cycle of MCF-7 at G 0/G 1 phase,while paclitaxel can block the cell cycle at G 2/M phase.After combined action,the number of cells blocked at G 2/M phase decreased.[Conclusions]Overall,the combined effect of psoralen and paclitaxel can enhance anti-tumor ability by inhibiting cell proliferation,inducing apoptosis,and blocking cell cycle.展开更多
Hereditary hearing loss is one of the most common neurosensory defects in humans. Approximately 70% of cases are nonsyndromic and could be inherited in autosomal domi- nant, autosomal recessive, mitochondrial, X-linke...Hereditary hearing loss is one of the most common neurosensory defects in humans. Approximately 70% of cases are nonsyndromic and could be inherited in autosomal domi- nant, autosomal recessive, mitochondrial, X-linked, and Y-linked manners （Wang et al., 2004; Alford, 2011）. The autosomal dominant type, comprising 15%-20% of non- syndromic hearing loss, is monogenic and genetically heterogeneous. Since the first dominant deafness locus （DFNA 1） was identified in 1992, a total of 64 DFNA loci have been mapped （DFNA1-DFNA64）,展开更多
基金Supported by the Natural Science Foundation Project of Guangxi University of Chinese Medicine(2020MS058)Youth Fund Project of Guangxi Natural Science Foundation(2022JJB140402)+4 种基金Basic Research Ability Improvement Project of Young and Middle-aged Teachers in Guangxi Universities(2022KY0278)"Youth Project"Talent Cultivation Project of Guangxi International Zhuang Medicine Hospital(2022001)High-level Key Discipline of Traditional Chinese Medicine(Zhuang Pharmacy)Construction Project of National Administration of Traditional Chinese Medicine(GZYYRJH[2022]226)Multidisciplinary Cross Innovation Team Project of Guangxi Traditional Chinese Medicine(GZKJ2309)"High-level Talent Cultivation and Innovation Team"Project of Guangxi University of Chinese Medicine(2022A008).
文摘[Objectives]To explore the effect of psoralen combined with paclitaxel on the apoptosis of MCF-7 cells.[Methods]The effects of different concentrations of psoralen,paclitaxel,or the combination of psoralen and paclitaxel on cell viability were detected using CCK-8 assay kit.Cell cycle distribution and apoptosis after 24 h of psoralen(0.16,0.32,0.64 mmol/L),paclitaxel(0.1μmol/L),combined action of psoralen(0.32 mmol/L)and paclitaxel(0.1μmol/L)were detected using flow cytometry.[Results]Lower concentration of psoralen(0.04-0.32 mmol/L)showed no significant inhibitory effect on cells.After combined with paclitaxel,the inhibitory effect on MCF-7 cell proliferation was significantly higher than that of the group treated alone.Compared with the paclitaxel group,the cell apoptosis rate in the drug combination group was significantly increased.Different low concentrations of psoralen can block the cell cycle of MCF-7 at G 0/G 1 phase,while paclitaxel can block the cell cycle at G 2/M phase.After combined action,the number of cells blocked at G 2/M phase decreased.[Conclusions]Overall,the combined effect of psoralen and paclitaxel can enhance anti-tumor ability by inhibiting cell proliferation,inducing apoptosis,and blocking cell cycle.
基金supported by the grants from the National Natural Science Foundation of China,key project(No.30830104) and major project(No.81120108009)
文摘Hereditary hearing loss is one of the most common neurosensory defects in humans. Approximately 70% of cases are nonsyndromic and could be inherited in autosomal domi- nant, autosomal recessive, mitochondrial, X-linked, and Y-linked manners （Wang et al., 2004; Alford, 2011）. The autosomal dominant type, comprising 15%-20% of non- syndromic hearing loss, is monogenic and genetically heterogeneous. Since the first dominant deafness locus （DFNA 1） was identified in 1992, a total of 64 DFNA loci have been mapped （DFNA1-DFNA64）,
