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The study of RNAi-mediated by conditionally replicating adenovirus silencing on Survivin gene in colon cancer cell lastingly
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作者 chunyi wang zhongxue fu 《The Chinese-German Journal of Clinical Oncology》 CAS 2008年第8期460-464,共5页
Objective: To investigate the effect of RNAj-mediated Survivin gene with conditionally replicating adenovirus silencing on Survivin gene in colon carcinoma cell lines HT-29 lastingly. Methods: We transfected Ad-delE... Objective: To investigate the effect of RNAj-mediated Survivin gene with conditionally replicating adenovirus silencing on Survivin gene in colon carcinoma cell lines HT-29 lastingly. Methods: We transfected Ad-delE1655KD-shRNA / Survivin-EGFP to HT-29 (control was replication defective adenovirus and liposome vector which was contained the same shRNA as Ad-delE1b55KD-shRNA / Survivin-EGFP). The expressions of EGFP, Survivin mRNA and Survivin protein in HT- 29 were detected at the 1st, 7th, 14th and 28th days alter transfection. Results: The expression of EGFP, the inhibition of Survivin mRNA and Survivin protein in HT-29 were high in each group at the 7th day alter transfection, among the total, the effect of Ad-delE1655KD-shRNA/Survivin-EGFP group was the highest; at the 14th day, the effects of replication defective adenovirus group and liposome vector group were decreased obviously, and it was still high in Ad-delE1655KD-shRNA / Survivin-EGFP group; at the 28th day, the effects of control groups were disappeared, and it was still high in Ad-delE1b55KD- shRNA/Survivin-EGFP group like before (P 〈 0.05). Conclusion: RNAi-mediated Survivin gene with conditionally replicating adenovirus can silence Survivin gene in colon carcinoma cell lines HT-29 lastingly. 展开更多
关键词 conditionally replicating adenovirus RNA interference SURVIVIN colon cancer APOPTOSIS
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