In the research described in this paper,the uses of the urea/thiourea complexation approach were employed to enhance the octane number of FCC gasoline by extracting n-alkanes.It was observed that adding thiourea impro...In the research described in this paper,the uses of the urea/thiourea complexation approach were employed to enhance the octane number of FCC gasoline by extracting n-alkanes.It was observed that adding thiourea improved the removal of the n-alkanes from gasoline,and matching results were obtained from experiments using model samples.Molecular dynamics simulation revealed that the stability of urea complexes increased as the carbon number of the n-alkanes was raised,whereas lighter n-alkane molecules exhibited a lower propensity for complex formation with urea.This finding is in agreement with the results of the DSC measurement at the decomposition temperature.Furthermore,infrared spectrum analysis,XRD characterization,and reaction heat measurements indicated that although thiourea was introduced into the reaction system,it did not actively participate in the complexation reaction.In summary,the introduction of thiourea resulted in an increased solubility of urea in an ethanol solution and enhanced the reaction heat,suggesting its beneficial role in promoting urea complex formation and facilitating n-alkane removal from FCC gasoline.展开更多
The onsite next generation sequencing(NGS)of Ebola virus(EBOV)genomes during the 2013–2016 Ebola epidemic in Western Africa provides an opportunity to trace the origin,transmission,and evolution of this virus.Herein,...The onsite next generation sequencing(NGS)of Ebola virus(EBOV)genomes during the 2013–2016 Ebola epidemic in Western Africa provides an opportunity to trace the origin,transmission,and evolution of this virus.Herein,we have diagnosed a cohort of EBOV patients in Sierra Leone in 2015,during the late phase of the outbreak.The surviving EBOV patients had a recovery process characterized by decreasing viremia,fever,and biochemical parameters.EBOV genomes sequenced through the longitudinal blood samples of these patients showed dynamic intra-host substitutions of the virus during acute infection,including the previously described short stretches of 13 serial TNC mutations.Remarkably,within individual patients,samples collected during the early phase of infection possessed Ts at these nucleotide sites,whereas they were replaced by Cs in samples collected in the later phase,suggesting that these short stretches of TNC mutations could emerge independently.In addition,up to a total of 35 nucleotide sites spanning the EBOV genome were mutated coincidently.Our study showed the dynamic intra-host adaptation of EBOV during patient recovery and gave more insight into the complex EBOV-host interactions.展开更多
基金The authors gratefully acknowledge the financial support from the key special project of“Inner Mongolia Revitalization Action with Science and Technology”(No.2021EEDSCXSFQZD004)from the Clean Combustion and Low-carbon Utilization of Coal Strategic Priority Research Program of the Chinese Academy of Sciences(Grant No.XDA 29000000)。
文摘In the research described in this paper,the uses of the urea/thiourea complexation approach were employed to enhance the octane number of FCC gasoline by extracting n-alkanes.It was observed that adding thiourea improved the removal of the n-alkanes from gasoline,and matching results were obtained from experiments using model samples.Molecular dynamics simulation revealed that the stability of urea complexes increased as the carbon number of the n-alkanes was raised,whereas lighter n-alkane molecules exhibited a lower propensity for complex formation with urea.This finding is in agreement with the results of the DSC measurement at the decomposition temperature.Furthermore,infrared spectrum analysis,XRD characterization,and reaction heat measurements indicated that although thiourea was introduced into the reaction system,it did not actively participate in the complexation reaction.In summary,the introduction of thiourea resulted in an increased solubility of urea in an ethanol solution and enhanced the reaction heat,suggesting its beneficial role in promoting urea complex formation and facilitating n-alkane removal from FCC gasoline.
基金supported by the Megaproject for Infectious Disease Research of China(2016ZX10004222-003)the research of Ebola pathogen from the National Natural Science Foundation of China(NSFC,81590763)+4 种基金National Key Research and Development Program of China(2016YFC1200200 to Y.Shu)the Distinguished Young Scientist Program of the NSFC(81525017 to Y.Shu)the Excellent Young Scientist Program of the NSFC(81822040 to W.J.Liu)the Taishan Scholar Project of Shandong Province(ts201511056 to W.Shi)G.F.Gao is a primary principal investigator of the NSFC Innovative Research Group(81621091).
文摘The onsite next generation sequencing(NGS)of Ebola virus(EBOV)genomes during the 2013–2016 Ebola epidemic in Western Africa provides an opportunity to trace the origin,transmission,and evolution of this virus.Herein,we have diagnosed a cohort of EBOV patients in Sierra Leone in 2015,during the late phase of the outbreak.The surviving EBOV patients had a recovery process characterized by decreasing viremia,fever,and biochemical parameters.EBOV genomes sequenced through the longitudinal blood samples of these patients showed dynamic intra-host substitutions of the virus during acute infection,including the previously described short stretches of 13 serial TNC mutations.Remarkably,within individual patients,samples collected during the early phase of infection possessed Ts at these nucleotide sites,whereas they were replaced by Cs in samples collected in the later phase,suggesting that these short stretches of TNC mutations could emerge independently.In addition,up to a total of 35 nucleotide sites spanning the EBOV genome were mutated coincidently.Our study showed the dynamic intra-host adaptation of EBOV during patient recovery and gave more insight into the complex EBOV-host interactions.
