We report here a fast staining method for single-stranded DNA fixed on nitrocellulose membrane using M-meso-letra(3-N-methylpyridyl)-porphrin (M-TMPyP, M: Mn3, Fe3, Pt3). The optimal staining solution consists of 54-1...We report here a fast staining method for single-stranded DNA fixed on nitrocellulose membrane using M-meso-letra(3-N-methylpyridyl)-porphrin (M-TMPyP, M: Mn3, Fe3, Pt3). The optimal staining solution consists of 54-108 μg/ml M-TMPyP in 25～200 mmol/l. phosphate buffer solution (pH 5.0～8.0).When DNA dot on NC membrane is treated with the staining solution at room temperature for 1～2 minutes with continuous stirring, it turns to brown-yellow.10pg per dot of single-stranded DNA is visible. It is a useful tool to sludy the DNA blotting, and for recognition of the special bands of DNA in a complete electrophoretogram of DNA blot. The staining mechanism is also discussed.The staining techniques of DNA play a important role in DNA blotting,display of DNA elcctophoretogram and location of the special DNA band from it.Since R.J. Fiel and co-workers[1] reported that meso-tetra(4-N-methylpyridyl) porphyrin can intercalale in calf thymus DNA. the intercalation between TMPyP-4 and DNA in solution has been intensively investigated by several groups[2～8],but none of them concerned with the interaction of metalloporphyrin and filterbound DNA. In this report, the use of M-TMPyP as a fast and sensitive staining reagent for single-strand DNA on NC membrane is described.展开更多
A new fluorometric method for the determination of sulfonamides is described, based on the formation of a fluorescent inclusion complex of carbonic anhydrase(CA) with dansylamide (DNSA). The fluorescence maximum emiss...A new fluorometric method for the determination of sulfonamides is described, based on the formation of a fluorescent inclusion complex of carbonic anhydrase(CA) with dansylamide (DNSA). The fluorescence maximum emission of the complex is at 460nm with excitation at 280nm. Dissociation constants were determined for the CA-sulfonamide complexes. Limits of detection for DNSA,sulfanilamide(SAN) and p-toluenesulfonamide (PTSN) were 0.84, 19.5 and 6.1 ng/ml, respectively. The recommended method, was applied. to the determination of sulfonamides in cow milk at ng/ml level.展开更多
文摘We report here a fast staining method for single-stranded DNA fixed on nitrocellulose membrane using M-meso-letra(3-N-methylpyridyl)-porphrin (M-TMPyP, M: Mn3, Fe3, Pt3). The optimal staining solution consists of 54-108 μg/ml M-TMPyP in 25～200 mmol/l. phosphate buffer solution (pH 5.0～8.0).When DNA dot on NC membrane is treated with the staining solution at room temperature for 1～2 minutes with continuous stirring, it turns to brown-yellow.10pg per dot of single-stranded DNA is visible. It is a useful tool to sludy the DNA blotting, and for recognition of the special bands of DNA in a complete electrophoretogram of DNA blot. The staining mechanism is also discussed.The staining techniques of DNA play a important role in DNA blotting,display of DNA elcctophoretogram and location of the special DNA band from it.Since R.J. Fiel and co-workers[1] reported that meso-tetra(4-N-methylpyridyl) porphyrin can intercalale in calf thymus DNA. the intercalation between TMPyP-4 and DNA in solution has been intensively investigated by several groups[2～8],but none of them concerned with the interaction of metalloporphyrin and filterbound DNA. In this report, the use of M-TMPyP as a fast and sensitive staining reagent for single-strand DNA on NC membrane is described.
文摘A new fluorometric method for the determination of sulfonamides is described, based on the formation of a fluorescent inclusion complex of carbonic anhydrase(CA) with dansylamide (DNSA). The fluorescence maximum emission of the complex is at 460nm with excitation at 280nm. Dissociation constants were determined for the CA-sulfonamide complexes. Limits of detection for DNSA,sulfanilamide(SAN) and p-toluenesulfonamide (PTSN) were 0.84, 19.5 and 6.1 ng/ml, respectively. The recommended method, was applied. to the determination of sulfonamides in cow milk at ng/ml level.
