Aim:The effects of hepatocyte growth factor(HGF)on a non-invasive prostate cancer cell line(CAHPV-10),expressing cMET were studied,to mimic the possible effects neo-adjuvant androgen deprivation therapy may have in pr...Aim:The effects of hepatocyte growth factor(HGF)on a non-invasive prostate cancer cell line(CAHPV-10),expressing cMET were studied,to mimic the possible effects neo-adjuvant androgen deprivation therapy may have in promoting tumour progression.Methods:Prostate epithelial cells and prostate cancer cells derived from cancer metastatic sites were analysed using cell culture assays,immunofluorescence,quantitative real-time polymerase chain reaction and western blotting,with or without HGF stimulation.Results:HGF significantly enhanced cell proliferation and induced cell scattering and invasion in CAHPV-10 cells compared to untreated controls.Active adenosine diphosphate-ribosylation factor 6(ARF6)was found to be present in all metastatic prostate cancer cells,with levels highest in the most aggressive cell line,PC-3.Following stimulation with HGF,active ARF6 expression was substantially elevated in CAHPV-10 cells.Conclusion:These findings provide further molecular insight into the progression of prostate cancer and highlights potential issues for early prostate cancer therapeutic strategies.展开更多
基金This work was funded by The St David's Medical Foundation and Prostate Action(grant reference number G2009/27).
文摘Aim:The effects of hepatocyte growth factor(HGF)on a non-invasive prostate cancer cell line(CAHPV-10),expressing cMET were studied,to mimic the possible effects neo-adjuvant androgen deprivation therapy may have in promoting tumour progression.Methods:Prostate epithelial cells and prostate cancer cells derived from cancer metastatic sites were analysed using cell culture assays,immunofluorescence,quantitative real-time polymerase chain reaction and western blotting,with or without HGF stimulation.Results:HGF significantly enhanced cell proliferation and induced cell scattering and invasion in CAHPV-10 cells compared to untreated controls.Active adenosine diphosphate-ribosylation factor 6(ARF6)was found to be present in all metastatic prostate cancer cells,with levels highest in the most aggressive cell line,PC-3.Following stimulation with HGF,active ARF6 expression was substantially elevated in CAHPV-10 cells.Conclusion:These findings provide further molecular insight into the progression of prostate cancer and highlights potential issues for early prostate cancer therapeutic strategies.
