Investigation of fungi responsible for the post harvest deterioration of Lycopersicum esculentus (tomatoes), Elaeis guineensis (palm fruit), Ipomoea batatas (sweet potato), Solanum tuberosum (Irish potato), Musa sapie...Investigation of fungi responsible for the post harvest deterioration of Lycopersicum esculentus (tomatoes), Elaeis guineensis (palm fruit), Ipomoea batatas (sweet potato), Solanum tuberosum (Irish potato), Musa sapientum (banana), Doucus carota (carrot), Musa paradisiaca (plantain), Carica papaya (pawpaw), Persea americana (Avocado pear), Citrullus lanatus (water-melon) and Capsicum chinense (fresh red pepper) from five different markets and farm lands in Enugu state, Nigeria was carried out. Healthy and diseased samples were collected from the selected markets/ farmlands. Fungal species found associated with the deterioration of the various fruits and vegetables tested included Mucor species (M. indicus, M. amphibiorum, M. racemosus and M. hiemalis), Rhizopus species (Rhizopus stolonifer, R. nigrican and R. oligosporus), Candida albicans, Aspergillus species (Aspergillus fumigatus, A. niger and A. flavus) and Penicillum species (P. oxalicum and P. chrysogenum) and Fusarium species (F. accuminatum, F. oxysporum, F. eqiuseti and F. moniliforme, F. solani, F. dimerum). All isolated fungi were pathogenic to the different fruits and vegetables from the result of pathogencity tests carried out.展开更多
The microbiological quality of sachet and tap water in Enugu,State,Nigeriawas analyzed. Sachet water was purchased from five different manufacturers. Samples Q1, T1, R1, J1, and M1 were directly from the manufacturers...The microbiological quality of sachet and tap water in Enugu,State,Nigeriawas analyzed. Sachet water was purchased from five different manufacturers. Samples Q1, T1, R1, J1, and M1 were directly from the manufacturers while samples Q2, T2, R2, J2, and M2 were obtained from the retailers. Tap water was collected from three different locations in the town. All water samples were subjected to bacteriological (aerobic and anaerobic) and fungal studies using standard bacteriological and mycological methods with little modifications. For sachet water, batch of five packets both from the manufacturer and retailers were analysed and average result taken. Five separate tap water samples were taken from the three different locations. For sachet water, E. coli and S. faecalis were isolated with colony forming units (CFU) ranging from 7 to >500. For tap water, isolates included Coliform, Cl. sp and Penicillium sp. An average of 66% Clostridium sp was recovered from all the tap water of the 3 different locations. Penicillium sp was isolated from only 16.6% of tap samples. Microbial quality may vary rapidly and widely but short-term peaks in pathogen concentration may increase disease risks considerably and may also trigger outbreaks of waterborne disease.展开更多
Salmonella bacilli infection has remained an important health problem in the developing world. The transmission of S. typhi most often occurs through food and water, which have been contaminated by faeces from carrier...Salmonella bacilli infection has remained an important health problem in the developing world. The transmission of S. typhi most often occurs through food and water, which have been contaminated by faeces from carriers and infected humans. Prevalence of carriers is a factor in transmission. The population under study lacked access to clean and safe water. Patients practiced self medication with inadequate dosing leading to relapsing infections and chronic carrier state. With this, there is a need to determine the actual dissemination index of the salmonella organism in the locality. The isolation from stool, blood and water samples were made using enrichment broths and selective media. Identification of isolates was carried out using standard methods. Characterization was done using O and H polyvalent sera. The total number of infected subjects within the 15 household was 22 (36.7%) among whom 10 (16.7%) were confirmed carriers. The total number/percentage infected by carrier contact was 9 (39.1%). Consumption of well water gave rise to the highest (60.0%) attack rate of typhoid fever infection. The continuance exposure to contaminated water source could be consistent to incessant and relapsing typhoid fever attacks in exposed individuals. Chronic carriers pose great risk to other members of the household, therefore regular screening is required.展开更多
文摘Investigation of fungi responsible for the post harvest deterioration of Lycopersicum esculentus (tomatoes), Elaeis guineensis (palm fruit), Ipomoea batatas (sweet potato), Solanum tuberosum (Irish potato), Musa sapientum (banana), Doucus carota (carrot), Musa paradisiaca (plantain), Carica papaya (pawpaw), Persea americana (Avocado pear), Citrullus lanatus (water-melon) and Capsicum chinense (fresh red pepper) from five different markets and farm lands in Enugu state, Nigeria was carried out. Healthy and diseased samples were collected from the selected markets/ farmlands. Fungal species found associated with the deterioration of the various fruits and vegetables tested included Mucor species (M. indicus, M. amphibiorum, M. racemosus and M. hiemalis), Rhizopus species (Rhizopus stolonifer, R. nigrican and R. oligosporus), Candida albicans, Aspergillus species (Aspergillus fumigatus, A. niger and A. flavus) and Penicillum species (P. oxalicum and P. chrysogenum) and Fusarium species (F. accuminatum, F. oxysporum, F. eqiuseti and F. moniliforme, F. solani, F. dimerum). All isolated fungi were pathogenic to the different fruits and vegetables from the result of pathogencity tests carried out.
文摘The microbiological quality of sachet and tap water in Enugu,State,Nigeriawas analyzed. Sachet water was purchased from five different manufacturers. Samples Q1, T1, R1, J1, and M1 were directly from the manufacturers while samples Q2, T2, R2, J2, and M2 were obtained from the retailers. Tap water was collected from three different locations in the town. All water samples were subjected to bacteriological (aerobic and anaerobic) and fungal studies using standard bacteriological and mycological methods with little modifications. For sachet water, batch of five packets both from the manufacturer and retailers were analysed and average result taken. Five separate tap water samples were taken from the three different locations. For sachet water, E. coli and S. faecalis were isolated with colony forming units (CFU) ranging from 7 to >500. For tap water, isolates included Coliform, Cl. sp and Penicillium sp. An average of 66% Clostridium sp was recovered from all the tap water of the 3 different locations. Penicillium sp was isolated from only 16.6% of tap samples. Microbial quality may vary rapidly and widely but short-term peaks in pathogen concentration may increase disease risks considerably and may also trigger outbreaks of waterborne disease.
文摘Salmonella bacilli infection has remained an important health problem in the developing world. The transmission of S. typhi most often occurs through food and water, which have been contaminated by faeces from carriers and infected humans. Prevalence of carriers is a factor in transmission. The population under study lacked access to clean and safe water. Patients practiced self medication with inadequate dosing leading to relapsing infections and chronic carrier state. With this, there is a need to determine the actual dissemination index of the salmonella organism in the locality. The isolation from stool, blood and water samples were made using enrichment broths and selective media. Identification of isolates was carried out using standard methods. Characterization was done using O and H polyvalent sera. The total number of infected subjects within the 15 household was 22 (36.7%) among whom 10 (16.7%) were confirmed carriers. The total number/percentage infected by carrier contact was 9 (39.1%). Consumption of well water gave rise to the highest (60.0%) attack rate of typhoid fever infection. The continuance exposure to contaminated water source could be consistent to incessant and relapsing typhoid fever attacks in exposed individuals. Chronic carriers pose great risk to other members of the household, therefore regular screening is required.