Gold Nanoparticle and Berberine Entrapped into Hydrogel Matrix as Drug Delivery System

In this study the novel hydrogel loaded with gold nanoparticle (AuNP) enhanced the berberine (BS) release when compared with other formulations of hydrogel. Hydrogels are hydrophilic polymer networks having the capacity to absorb water, ranging from about twenty to thousand times their dry weight. BS is a natural product, a quaternary ammonium salt from the group of isoquinoline alkaloids found in medicinal plants as Berberis Vulgaris. BS has some activity against dysentery, hypertension, inflammation, and liver disease in China and Japan. In this work, BS was used as a model drug to study its association with different types of hydrogel composites of polyvinyl alcohol (BS-PVA 10%);gellan gum (BS-GG 2%), gellan gum-PVA crosslinked with cysteine (cys) (BSGG2%PVA2%cys) and gellan gum-PVA cosslinked with cysteine associated with gold nanoparticles (AuNP-BSGG2%PVA2%cys). Several parameters such as fraction of retained water (Wf), hydration percentage (%H), Swelling (DSw) and time course profile (t = 100%) (TC) were evaluated for all preparations. The results showed that the AuNP-BS-GG2%PVA2%cys was able to retain more water and swelling than the other preparations. The time course of release of the BS to the medium was greater for AuNP-BS-GG2%PVA2%cys making it a candidate to drug delivery studies in biological assays. Also Scanning Electron Microscopy (SEM) images of the surface of these hydrogel were performed. Furthermore, crosslink of the resulting hydrogels were investigated by Fourier Transform Infrared Spectroscopy (FTIR) and differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA). Thus, briefly, the aim of this work was to study three composition of hydrogel loaded with BS and its composition in relation to addition to AuNP and evaluate its profile for further drug delivery application using the Surface Plasmonic Resonance (SPR) as a tool improving the drug release in the new hydrogel.

Chlorambucil Encapsulation into PLGA Nanoparticles and Cytotoxic Effects in Breast Cancer Cell

The present work aimed to develop and evaluate a colloidal system composed of poly (DL-lactide-co-glycolide) (PLGA) nanoparticles (NPs) associated with chlorambucil (CHB) and its effects on cancer cells. The nanoparticles showed %EE (>92%), a mean particle size in the range of 240 to 334 nm and zeta potential of -16.7 to -26.0 mV. In vitro release profile showed a biphasic pattern, with an initial burst for all formulations. The scanning electron microscopy of CHB-nanoparticles showed regular spherical shapes, smooth surface without aggregations. Differential scanning calorimetry thermograms, UV-vis absorption, fluorescence emission and Fourier transform infrared spectroscopy were performed showing the entrapment of the antitumoral in drug delivery system. CHB encapsulated in PLGA nanoparticles decrease the survival rates of the breast cancer cells: 68.9% reduction of cell viability on MCF-7 cell line and 59.7% on NIH3T3. Our results indicated that polymeric nanoparticles produced by classical methods are efficient drug delivery systems for CHB.