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肿瘤免疫治疗时代的多重免疫组织化学/免疫荧光技术 被引量:4
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作者 Wei Chang colin tan +7 位作者 Sanjna Nilesh Nerurkar Hai Yun Cai Harry Ho Man Ng Duoduo Wu Yu Ting Felicia Wee Jeffrey Chun Tatt Lim Joe Yeong Tony Kiat Hon Lim 《癌症》 SCIE CAS 2021年第9期367-387,共21页
常规免疫组织化学(immunohistochemistry,IHC)是组织病理学中广泛应用的诊断技术。但该技术存在诸多局限性,包括观察者间的变异性高,每张组织切片仅能标记一个标志物。本文详细描述了各种多重检测技术,这些技术克服了上述局限性,可在单... 常规免疫组织化学(immunohistochemistry,IHC)是组织病理学中广泛应用的诊断技术。但该技术存在诸多局限性,包括观察者间的变异性高,每张组织切片仅能标记一个标志物。本文详细描述了各种多重检测技术,这些技术克服了上述局限性,可在单张切片上同时检测多个标志物,可对细胞组成、细胞功能和细胞间相互作用进行整合研究。在这些技术中,多重免疫组织化学/免疫荧光(multiplex immunohistochemistry/immunofluorescence,mIHC/IF)已成为最具应用前景的技术。mIHC/IF为开展高重复性、高效、高性价比的组织研究提供了高通量多重染色技术和标准化的定量分析。该技术在转化研究和临床实践中,尤其是肿瘤免疫治疗中极具应用价值。 展开更多
关键词 免疫荧光 免疫组织化学 免疫治疗 多重 概述
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Building a Machine Learning-based Ambulance Dispatch Triage Model for Emergency Medical Services
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作者 Han Wang Qin Xiang Ng +3 位作者 Shalini Arulanandam colin tan Marcus E.H.Ong Mengling Feng 《Health Data Science》 2023年第1期17-25,共9页
Background:In charge of dispatching the ambulances,Emergency Medical Services(EMS)call center specialists often have difficulty deciding the acuity of a case given the information they can gather within a limited time... Background:In charge of dispatching the ambulances,Emergency Medical Services(EMS)call center specialists often have difficulty deciding the acuity of a case given the information they can gather within a limited time.Although there are protocols to guide their decision-making,observed performance can still lack sensitivity and specificity.Machine learning models have been known to capture complex relationships that are subtle,and well-trained data models can yield accurate predictions in a split of a second.Methods:In this study,we proposed a proof-of-concept approach to construct a machine learning model to better predict the acuity of emergency cases.We used more than 360,000 structured emergency call center records of cases received by the national emergency call center in Singapore from 2018 to 2020.Features were created using call records,and multiple machine learning models were trained.Results:A Random Forest model achieved the best performance,reducing the over-triage rate by an absolute margin of 15%compared to the call center specialists while maintaining a similar level of under-triage rate.Conclusions:The model has the potential to be deployed as a decision support tool for dispatchers alongside current protocols to optimize ambulance dispatch triage and the utilization of emergency ambulance resources. 展开更多
关键词 Services ABSOLUTE PROOF
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Eventual Positivity of Hermitian Polynomials and Integral Operators 被引量:1
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作者 colin tan 《Chinese Annals of Mathematics,Series B》 SCIE CSCD 2016年第1期83-94,共12页
Quillen proved that if a Hermitian bihomogeneous polynomial is strictly positive on the unit sphere, then repeated multiplication of the standard sesquilinear form to this polynomial eventually results in a sum of Her... Quillen proved that if a Hermitian bihomogeneous polynomial is strictly positive on the unit sphere, then repeated multiplication of the standard sesquilinear form to this polynomial eventually results in a sum of Hermitian squares. Catlin-D'Angelo and Varolin deduced this positivstellensatz of Quillen from the eventual positive-definiteness of an associated integral operator. Their arguments involve asymptotic expansions of the Bergman kernel. The goal of this article is to give an elementary proof of the positive-definiteness of this integral operator. 展开更多
关键词 ASYMPTOTICS POLYNOMIAL POSITIVITY
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Eventual Positivity of Hermitian Algebraic Functions and Associated Integral Operators
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作者 colin tan Wing-Keung TO 《Chinese Annals of Mathematics,Series B》 SCIE CSCD 2020年第6期967-988,共22页
Quillen proved that repeated multiplication of the standard sesquilinear form to a positive Hermitian bihomogeneous polynomial eventually results in a sum of Hermitian squares,which was the first Hermitian analogue of... Quillen proved that repeated multiplication of the standard sesquilinear form to a positive Hermitian bihomogeneous polynomial eventually results in a sum of Hermitian squares,which was the first Hermitian analogue of Hilbert s seventeenth problem in the nondegenerate case.Later Catlin-D’Angelo generalized this positivstellensatz of Quillen to the case of Hermitian algebraic functions on holomorphic line bundles over compact complex manifolds by proving the eventual positivity of an associated integral operator.The arguments of Catlin-D’Angelo involve subtle asymptotic estimates of the Bergman kernel.In this article,the authors give an elementary and geometric proof of the eventual positivity of this integral operator,thereby yielding another proof of the corresponding positivstellensatz. 展开更多
关键词 Hermitian algebraic functions Integral operators POSITIVITY
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