Lipopolysaccharides protect mesenchymal stem cell against cardiac ischemia-reperfusion injury by HMGB1/STAT3 signaling

BACKGROUND Myocardial ischemia-reperfusion(I/R) is a serious and irreversible injury. Bone marrow-derived mesenchymal stem cells(MSCs) is considered to be a potential therapy for I/R injury due to the paracrine effects. High-mobility group box1(HMGB1) is a novel mediator in MSC and regulates the response of inflammation injury. Signal Transduction and Transcription Activator 3(STAT3) is a critical transcription factor and important for release of paracrine factors. However, the relationship between HMGB1 and STAT3 in paracrine effect of MSC remains unknown.METHODS In vitro, hypoxia/reoxygenation injury model was established by Anaero Pack System and examined by Annexin V flow cytometry, CCK8 assay and morphology observation. Detection of apoptotic proteins and protein expression of HMGB1and STAT3 by Western blot.RESULTS The conditioned medium of MSCs with or without LPS pretreatment was cocultured with H9C2 cells for 24 h before hypoxia treatment and MSC showed obvious cardiomyocytes protect role, as evidence by decreased apoptosis rate and improved cells viability, and LPS pretreated MSC exhibited better protect role than untreated MSC. However, such effect was abolished in HMGB1 deficiency group, silencing HMGB1 decreased the secretion of vascular endothelial growth factor(VEGF), hepatocyte growth factor(HGF), insulin growth factor(IGF), cell viability, and the expression of STAT3. Furthermore, STAT3 silence attenuated the protective effect of LPS in MSC.CONCLUSIONS These findings suggested that LPS improved MSC-mediated cardiomyocytes protection by HMGB1/STAT3signaling.

基于MAS的智能数控系统体系结构研究（英文）

作为智能制造的技术基础与核心控制单元,智能数控系统表现出信息感知、交互以及自主决策优化等特性。利用MAS进行智能数控系统体系结构设计,可以充分发挥Agent在学习推理以及智能决策等方面的优势,满足系统智能化需求。在分析智能数控加工流程的基础上,提出采用层次结构设计,按系统功能划分为人机交互、信息管理、几何设计、工艺规划、运动控制、逻辑控制以及故障诊断等模块。各模块间采用混合式结构进行通信。

Exploring the tidal effect of urban business district with large-scale human mobility data

Business districts are urban areas that have various functions for gathering people,such as work,consumption,leisure and entertainment.Due to the dynamic nature of business activities,there exists significant tidal effect on the boundary and functionality of business districts.Indeed,effectively analyzing the tidal patterns of business districts can benefit the economic and social development of a city.However,with the implicit and complex nature of business district evolution,it is non-trivial for existing works to support the fine-grained and timely analysis on the tidal effect of business districts.To this end,we propose a data-driven and multi-dimensional framework for dynamic business district analysis.Specifically,we use the large-scale human trajectory data in urban areas to dynamically detect and forecast the boundary changes of business districts in different time periods.Then,we detect and forecast the functional changes in business districts.Experimental results on real-world trajectory data clearly demonstrate the effectiveness of our framework on detecting and predicting the boundary and functionality change of business districts.Moreover,the analysis on practical business districts shows that our method can discover meaningful patterns and provide interesting insights into the dynamics of business districts.For example,the major functions of business districts will significantly change in different time periods in a day and the rate and magnitude of boundaries varies with the functional distribution of business districts.

Preparation and Degradation Phenol Characterization of Ti/SnO_2-Sb-Mo Electrode Doped with Different Contents of Molybdenum

The Ti/SnO2-Sb-Mo electrodes doped with different molar ratios of molybdenum（Mo） were prepared by sol—gel method in order to investigate the effect of Mo on the characterization of Ti/SnO2-Sb—Mo electrodes.X-ray diffraction（XRD）,field-emission scanning electron microscopy（FE-SEM）,energy dispersive spectrometry（EDS）,and linear scanning voltammetry（LSV） were used to scrutinize the coating material and the electrochemical activity.The concentration of phenol,the value of total organic carbon（TOC）,the mineralization current efficiency（MCE） and the ultraviolet—visible spectroscopy（UV-Vis） spectrum of phenol solution were measured over the electrochemical degradation process of phenol to confirm the phenol degradation characterization of Ti/SnO2-Sb-Mo electrodes.Results showed that the electrode at the Mo content of 1 at.%provided optimal catalytic activity for phenol degradation and the longest life time.The removal percentage of phenol and TOC were 99.62%and82.67%,respectively.The Ti/SnO2-Sb-Mo electrode with 1 at.%of Mo reached maximum MCE of phenol oxidation.The kinetic investigation of phenol and TOC degradation displayed the pseudo-first order reaction model.The Ti/SnO2-Sb-Mo electrode coating with 7 at.%Mo presented the highest oxygen evolution overpotential,indicating the diverse effects for different Mo molar ratio doping.

A new ginsengenin containing an oxacyclopentane-ring isolated from the acid hydrolysate of total ginsenosides

Bioassay-guided fractionation of the acid hydrolysate of total ginsenosides of Panax ginseng C.A.Meyer （Araliaceae） led to the isolation of a novel ginsengenin（1）.The structure of 1 was determined as （20S,22S）-dammar-22,25-epoxy-3β,12β,20-triol by extensive spectroscopy and single-crystal X-ray diffraction analyses.The cytotoxicity of 1 was further tested against SWl 116,HCTl 16,and A549 cells by the MTT method,with IC50 values in the range of 2.96-30.9μmol/L.