The conformation of silk fibroin （SF） frozen with polyethylene glycol （PEG） at a molecular weight from 2 kDa to 20 kDa and a mass ratio of PEG：SF from 1：5 to 10：1 was studied by spectral and microscopic methods...The conformation of silk fibroin （SF） frozen with polyethylene glycol （PEG） at a molecular weight from 2 kDa to 20 kDa and a mass ratio of PEG：SF from 1：5 to 10：1 was studied by spectral and microscopic methods. It is found that the conformation transition of SF from random coil to β-sheet could be induced by the stress resulting from PEG crystallization at -20 ℃, and greatly depended on the cooling rate, PEG：SF mass ratio and PEG molecular weight. These findings provide a new method for the preparation of desired SF nanofibers.展开更多
基金financially supported by the National Natural Science Foundation of China(No.21374022)
文摘The conformation of silk fibroin （SF） frozen with polyethylene glycol （PEG） at a molecular weight from 2 kDa to 20 kDa and a mass ratio of PEG：SF from 1：5 to 10：1 was studied by spectral and microscopic methods. It is found that the conformation transition of SF from random coil to β-sheet could be induced by the stress resulting from PEG crystallization at -20 ℃, and greatly depended on the cooling rate, PEG：SF mass ratio and PEG molecular weight. These findings provide a new method for the preparation of desired SF nanofibers.
