Ammonia-oxidizing archaea(AOA)play crucial roles in marine carbon and nitrogen cycles by fixing inorganic carbon and performing the initial step of nitrification.Evaluation of carbon and nitrogen metabolism popularly ...Ammonia-oxidizing archaea(AOA)play crucial roles in marine carbon and nitrogen cycles by fixing inorganic carbon and performing the initial step of nitrification.Evaluation of carbon and nitrogen metabolism popularly relies on functional genes such as amoA and accA.Increasing studies suggest that quorum sensing(QS)mainly studied in biofilms for bacteria may serve as a universal communication and regulatory mechanism among prokaryotes;however,this has yet to be demonstrated in marine planktonic archaea.To bridge this knowledge gap,we employed a combination of metabolic activity markers(amoA,accA,and grs)to elucidate the regulation of AOA-mediated nitrogen,carbon processes,and their interactions with the sur-rounding heterotrophic population.Through co-transcription investigations linking metabolic markers to potential key QS genes,we discovered that QS molecules could regulate AOA's carbon,nitrogen,and lipid metabolisms under different conditions.Interestingly,specific AOA ecotypes showed a preference for employing distinct QS systems and a distinct QS circuit involving a typical population.Overall,our data demonstrate that Qs orchestrates nitrogen and carbon metabolism,including the exchange of organic metabolites between AOA and surrounding heterotrophic bacteria,which has been pre-viously overlooked in marine AOA research.展开更多
基金This work was supported by the National Natural Science Foundation of China(Nos.42321004 and 92351301)the Stable Support Plan Program of Shenzhen Natural Science Fund(20200925173954005)+3 种基金the Shenzhen Municipal Commission of Science and Technology Innovation(KCXFZ20211020174803005)the Guangdong Basic and Applied Basic Research Foundation(2021B 1515120080 and 2020B1515120012)the Shenzhen Key Laboratory of Marine Archaea Geo-Omics,Southern University of Science and Technology(ZDSYS201802081843490)the Agence Nationale de la Recherche(ANR)through the projects EUREKA(ANR-14-CE02-0004-01).
文摘Ammonia-oxidizing archaea(AOA)play crucial roles in marine carbon and nitrogen cycles by fixing inorganic carbon and performing the initial step of nitrification.Evaluation of carbon and nitrogen metabolism popularly relies on functional genes such as amoA and accA.Increasing studies suggest that quorum sensing(QS)mainly studied in biofilms for bacteria may serve as a universal communication and regulatory mechanism among prokaryotes;however,this has yet to be demonstrated in marine planktonic archaea.To bridge this knowledge gap,we employed a combination of metabolic activity markers(amoA,accA,and grs)to elucidate the regulation of AOA-mediated nitrogen,carbon processes,and their interactions with the sur-rounding heterotrophic population.Through co-transcription investigations linking metabolic markers to potential key QS genes,we discovered that QS molecules could regulate AOA's carbon,nitrogen,and lipid metabolisms under different conditions.Interestingly,specific AOA ecotypes showed a preference for employing distinct QS systems and a distinct QS circuit involving a typical population.Overall,our data demonstrate that Qs orchestrates nitrogen and carbon metabolism,including the exchange of organic metabolites between AOA and surrounding heterotrophic bacteria,which has been pre-viously overlooked in marine AOA research.
