Cytosine methylation/demethylation plays pivotal roles in regulating gene expression at a genome-wide level. However, limited reports are available to reveal correlating changes of cytosine methylation and proteomic e...Cytosine methylation/demethylation plays pivotal roles in regulating gene expression at a genome-wide level. However, limited reports are available to reveal correlating changes of cytosine methylation and proteomic expression in Brassica napus so far. Therefore, in the present study, global cytosine methylation and proteome were analysed in B. napus after cold treatment by methylation-sensitive amplified polymorphism （MSAP） and two-dimensional protein electrophoresis technology （2-DE）. The results showed that the lowered genome-wide DNA methylation status was revealed after cold treatment, and about 0.88% of discrepancy in DNA methylation was detected between the non-flowering and flowering plants after cold treatment. Moreover, the 52 significantly up-regulated proteins emerged in comparison with the 36 down-regulated proteins, as well as the 14 proteins exclusively detected in the flowering plants. Intriguingly the 8 specifically expressed proteins in the non-flowering plants disappeared in the flowering plants with cold treatment. Therefore, these present data proved that the correlating changes of cytosine methylation and proteomic expression were evidenced under cold treatment in B. napus.展开更多
We investigate the evolutionary Prisoner＇s dilemma game on the simplest spatial networks constructed as geometrical graphs. The optimal cooperation enhancement against the topology randomness is found. It is proposed...We investigate the evolutionary Prisoner＇s dilemma game on the simplest spatial networks constructed as geometrical graphs. The optimal cooperation enhancement against the topology randomness is found. It is proposed that the optimal behavior of the cooperation results from the competition between individuals with high degrees and with low degrees： the former assists the formation of cooperator clusters and the latter tends to prevent the formation of such dusters.展开更多
基金the National Key Technology R&D Program of China(2010BAD01B02)the foundation of Postgraduate Research Program of Zhengzhou University,China(12L00403)
文摘Cytosine methylation/demethylation plays pivotal roles in regulating gene expression at a genome-wide level. However, limited reports are available to reveal correlating changes of cytosine methylation and proteomic expression in Brassica napus so far. Therefore, in the present study, global cytosine methylation and proteome were analysed in B. napus after cold treatment by methylation-sensitive amplified polymorphism （MSAP） and two-dimensional protein electrophoresis technology （2-DE）. The results showed that the lowered genome-wide DNA methylation status was revealed after cold treatment, and about 0.88% of discrepancy in DNA methylation was detected between the non-flowering and flowering plants after cold treatment. Moreover, the 52 significantly up-regulated proteins emerged in comparison with the 36 down-regulated proteins, as well as the 14 proteins exclusively detected in the flowering plants. Intriguingly the 8 specifically expressed proteins in the non-flowering plants disappeared in the flowering plants with cold treatment. Therefore, these present data proved that the correlating changes of cytosine methylation and proteomic expression were evidenced under cold treatment in B. napus.
文摘We investigate the evolutionary Prisoner＇s dilemma game on the simplest spatial networks constructed as geometrical graphs. The optimal cooperation enhancement against the topology randomness is found. It is proposed that the optimal behavior of the cooperation results from the competition between individuals with high degrees and with low degrees： the former assists the formation of cooperator clusters and the latter tends to prevent the formation of such dusters.
