目的分析血清Occludin与基底核区脑出血量、严重程度以及认知功能障碍的关系。方法回顾性收集2019年2月~2021年2月于笔者医院首次确诊基底核区脑出血118例患者作为观察组,同期选择健康体检者90例作为对照组。酶联免疫吸附实验(enzyme li...目的分析血清Occludin与基底核区脑出血量、严重程度以及认知功能障碍的关系。方法回顾性收集2019年2月~2021年2月于笔者医院首次确诊基底核区脑出血118例患者作为观察组,同期选择健康体检者90例作为对照组。酶联免疫吸附实验(enzyme linked immunosorbent assay,ELISA)检测血清Occludin。结果与对照组比较,观察组血清Occludin显著升高(4.56±1.03ng/ml vs 1.96±0.42ng/ml,P<0.001)。根据出血量将观察组分为<20ml组和20~40ml组,20~40ml组血清Occludin显著高于<20ml组(6.12±1.34ng/ml vs 3.81±1.22ng/ml,P<0.001)。根据美国国立卫生研究院卒中量表(National Institutes of Health Stroke Scale,NIHSS)评分标准将观察组分为轻度51例(0~6分)、中度45例(7~15分)和重度22例(≥16分),与轻度患者(3.76±1.02ng/ml)比较,重度(6.03±0.89ng/ml)和中度(5.86±1.1ng/ml)患者血清Occludin显著升高(P<0.001)。治疗90天后应用蒙特利尔认知评估表(Montreal Cognitive Assessment,MoCA)和简易精神状态评价量表(minimental state assessment scale,MMSE)进行认知功能评估,认知功能障碍患者(n=48)比无认知功能障碍患者(n=70)血清Occludin显著升高(6.35±1.64ng/ml vs 3.55±1.19ng/ml,P<0.001)。受试者工作特征(receiver operating characteristic,ROC)曲线显示,血清Occludin评估认知功能障碍的曲线下面积(area under curve,AUC)为0.779,临界值为4.95ng/ml,敏感度为77.5%,特异性为70.9%。多因素Logistic回归分析发现,年龄≥65岁、NIHSS评分≥7分、出血量≥20ml、血清Occludin≥5.0ng/ml是认知功能障碍的独立危险因素(P<0.05)。结论基底核区脑出血患者血清Occludin水平升高与出血量、严重程度以及认知功能障碍密切相关,可作为临床重要生化标志物。展开更多
Traditional community ecology focuses on the nutruitional relations at the levels of food chain or food web,while chemical ecology focuses on the communication among organisms based on chemical information.The full in...Traditional community ecology focuses on the nutruitional relations at the levels of food chain or food web,while chemical ecology focuses on the communication among organisms based on chemical information.The full integration between these two fields will improve our understanding of insect behaviors.This paper reviews infochemicals from different sources and their interactions that affect insect behaviors in natural ecosystems.展开更多
An expedient and efficient method for selective methylation of catechol coumarins by working with different alkalis under appropriate reaction conditions was reported. Esculetins were selectively methylated at positio...An expedient and efficient method for selective methylation of catechol coumarins by working with different alkalis under appropriate reaction conditions was reported. Esculetins were selectively methylated at position 6 and position 7 in good yields using CH3I in the presence of Nail and Na2CO3, respectively. However, daphnetins showed less regioselectivity under the same reaction conditions. Furthermore, the site preference for the methylation reaction was interpreted by the density functional theory at B3LYP/6-31 ＋G（d） level.展开更多
文摘目的分析血清Occludin与基底核区脑出血量、严重程度以及认知功能障碍的关系。方法回顾性收集2019年2月~2021年2月于笔者医院首次确诊基底核区脑出血118例患者作为观察组,同期选择健康体检者90例作为对照组。酶联免疫吸附实验(enzyme linked immunosorbent assay,ELISA)检测血清Occludin。结果与对照组比较,观察组血清Occludin显著升高(4.56±1.03ng/ml vs 1.96±0.42ng/ml,P<0.001)。根据出血量将观察组分为<20ml组和20~40ml组,20~40ml组血清Occludin显著高于<20ml组(6.12±1.34ng/ml vs 3.81±1.22ng/ml,P<0.001)。根据美国国立卫生研究院卒中量表(National Institutes of Health Stroke Scale,NIHSS)评分标准将观察组分为轻度51例(0~6分)、中度45例(7~15分)和重度22例(≥16分),与轻度患者(3.76±1.02ng/ml)比较,重度(6.03±0.89ng/ml)和中度(5.86±1.1ng/ml)患者血清Occludin显著升高(P<0.001)。治疗90天后应用蒙特利尔认知评估表(Montreal Cognitive Assessment,MoCA)和简易精神状态评价量表(minimental state assessment scale,MMSE)进行认知功能评估,认知功能障碍患者(n=48)比无认知功能障碍患者(n=70)血清Occludin显著升高(6.35±1.64ng/ml vs 3.55±1.19ng/ml,P<0.001)。受试者工作特征(receiver operating characteristic,ROC)曲线显示,血清Occludin评估认知功能障碍的曲线下面积(area under curve,AUC)为0.779,临界值为4.95ng/ml,敏感度为77.5%,特异性为70.9%。多因素Logistic回归分析发现,年龄≥65岁、NIHSS评分≥7分、出血量≥20ml、血清Occludin≥5.0ng/ml是认知功能障碍的独立危险因素(P<0.05)。结论基底核区脑出血患者血清Occludin水平升高与出血量、严重程度以及认知功能障碍密切相关,可作为临床重要生化标志物。
基金Supported by National Natural Science Foundation of China(31071972).
文摘Traditional community ecology focuses on the nutruitional relations at the levels of food chain or food web,while chemical ecology focuses on the communication among organisms based on chemical information.The full integration between these two fields will improve our understanding of insect behaviors.This paper reviews infochemicals from different sources and their interactions that affect insect behaviors in natural ecosystems.
基金Supported by the National Basic Research Program of China（No.2013CB531800）, the National Natural Science Foundation of China（Nos.81402822, 81302793） and the Science and Technology Project of Liaoning Province of China（No.2014204007）.
文摘An expedient and efficient method for selective methylation of catechol coumarins by working with different alkalis under appropriate reaction conditions was reported. Esculetins were selectively methylated at position 6 and position 7 in good yields using CH3I in the presence of Nail and Na2CO3, respectively. However, daphnetins showed less regioselectivity under the same reaction conditions. Furthermore, the site preference for the methylation reaction was interpreted by the density functional theory at B3LYP/6-31 ＋G（d） level.
