An HPLC Fingerprint Identification of Dried Barks of Ilex rotunda and Ilex godajam

[Objectives] A simple and reliable HPLC fingerprint method was developed for the identification of dried barks of Ilex rotunda and I. godajam. [Methods] Nine batches of dried barks of I. rotunda,and seven batches of dried barks of I. godajam collected from different pharmacies and arboretums in different regions of China were used to establish fingerprints. The software Similarity Evaluation System of Chromatographic Fingerprints of Traditional Chinese Medicine( 2004 A Edition) was used to evaluate the fingerprints. [Results]The fingerprints of dried barks of I. rotunda and I. godajam were established. Methodological study met the technical requirements of fingerprints. The similarities of the fingerprints of dried barks of I. rotunda and I. godajam were all more than 0. 8 and 0. 9 respectively. There were 31 and 28 common peaks in I. rotunda and I. godajam,which could be classified into two clusters by principal component analysis( PCA) and hierarchical cluster analysis. [Conclusions] The feasibility and advantages of used HPLC fingerprints were verified,and the results indicated that the HPLC fingerprint as a characteristic distinguishing method combining similarity evaluation,principal component analysis and hierarchical cluster analysis can be successfully used to identify the authenticity of dried barks of I. rotunda and I. godajam.

System Control Research of Multi-link Fructus Trichosanthis Quality

It could not guarantee the quality of medicinal materials and decoction pieces to purely make quality standard of traditional Chinese medicine and carry out quality control. For the problems existed in each process and link of traditional Chinese medicine production,control techniques and measures of guaranteeing and improving quality should be proposed,thereby forming process control system for quality of each traditional Chinese medicine. Based on systemic research on Fructus Trichosanthis which is common traditional Chinese medicine more than30 years,the concept of establishing process control system of Fructus Trichosanthis quality is proposed firstly in this paper. The research aims to find quality problems from 10 processes and links( plant variety,cultivation and propagation,field pest control,fruiting and maturity degree of fruit,storage,regulator,chemical ingredient,quality standard,limited index and circulation),and form feasible techniques and measures solving problems in each process and link,thereby systemically guaranteeing the quality of Fructus Trichosanthis medicinal material and decoction pieces. It guarantees and improves clinical efficacy of Fructus Trichosanthis in Chinese medicine,and could provide reference for establishing process control system of quality of other traditional Chinese medicines.

Correlation between 5-hydroxymethylfurfural content and color of Rehmanniae Radix and Rehmanniae Radix Praeparata

In the present study,we explored the correlation between 5-hydroxymethylfurfural(5-HMF)and color of Rehmanniae Radix(RR)and Rehmanniae Radix Praeparata(RRP).The color was observed by human eyes under sunlight.The chromatic value and color difference were detected by a colorimeter.The water content was determined by an oven-drying method.The 5-HMF content was determined by an HPLC method.The correlation between the 5-HMF content and the color was analyzed by bivariate correlation analysis.The results showed that the color was greyish-brown and dark brown for RR,and it was black for RRP.The deepening color of RRP was reflected in the decrease of L^*,a^*and b^*values.The water content was in accord with the requirement of Chinese Pharmacopoeia.The 5-HMF contents of RR and RRP were 0.9711–25.71μg/g and 213.4–4010μg/g,respectively.The average 5-HMF contents of RR and RRP were 8.059μg/g and 1433μg/g,respectively.The 5-HMF content in RRP was higher than that in RR.The correlations between 5-HMF content and L^*,a^*and b^*values were significant.The color of RRP was deeper than that of RR.The 5-HMF content of RRP was higher than 0.02%,while it was less than 0.02%of RR,which could be used as a marker component for judging RR and RRP.

Crystal structure of trans-3-phenyl-2-propenoic acid

The title compound of trans-3-phenyl-2-propenoic acid （C9H802）, a main active organic acid of cinnamon bark and styrax, was obtained by oxidization from trans-3-phenyl-2-propenaldehyde and characterized by X-ray diffraction analysis. It crystallizes in monoclinic system, space group P21/n with a = 5.55916（10）, b = 17.4870（3）, c = 7.70677（13） A, V = 744.89（2） A3, Z= 4, Dc= 1.321 g/cm3, Mr = 148.15, F（000） = 312, and/t μ=0.764 mm-1. A total of 2661 reflections with 1447 unique ones （Rint = 0.0137） were collected, in which 1447 were observed （I〉2σ（/））. There were two C9H802 molecules in an asymmetric unit. The structural unit was a dimer formed by the hydrogen bonding of the oxygen atom of carbonyl group and the hydrogen atom of hydroxyl group. A 3D layer structure was formed through hydrogen bonds and Van der Walls＇ forces.