T-2 toxin is the most widespread mycotoxin in crops,feed and food,which poses a serious threat to body health.Bone is the main target tissue for T-2 toxin accumulation.Ingestion of food contaminated by T-2 toxin is th...T-2 toxin is the most widespread mycotoxin in crops,feed and food,which poses a serious threat to body health.Bone is the main target tissue for T-2 toxin accumulation.Ingestion of food contaminated by T-2 toxin is the main cause of Kashin-Beck disease.However,the specific mechanism of bone damage caused by T-2 toxin is still unclear.In this study,a total of 40 male C57BL/6N mice were divided into four groups and orally treated with 0,0.5,1.0 and 2.0 mg·kg^(-1) body weight T-2 toxin for 28 days.The results showed that exposure to T-2 toxin led to weight loss,bone mineral density reduction and femoral structural damage of mice.In addition,osteoblast-mediated bone formation was inhibited,and osteoclast-mediated bone resorption was enhanced.Meanwhile,the levels of bone metabolism-related hormones including parathyroid hormone,calcitonin and 1,25-dihydroxyvitamin D3 were reduced.More importantly,it was found that the level of neuropeptide Y(a neurohormone)was decreased.These results provided a new perspetive for understanding the osteotoxicity of T-2 toxin.展开更多
目的比较通辽地区不同来源奇异变形杆菌对小鼠致病力及其毒力基因携带情况。方法取4株(SPM、GPM、BPM-1和BPM-2)不同来源的奇异变形杆菌,进行生化鉴定、药物敏感性测试及动物致病性试验,对4株菌株的8种毒力基因(ureC、zapA、mrpA、ucaA...目的比较通辽地区不同来源奇异变形杆菌对小鼠致病力及其毒力基因携带情况。方法取4株(SPM、GPM、BPM-1和BPM-2)不同来源的奇异变形杆菌,进行生化鉴定、药物敏感性测试及动物致病性试验,对4株菌株的8种毒力基因(ureC、zapA、mrpA、ucaA、rsbA、pmfA、atfA、atfC)进行PCR检测,对其16S r RNA基因序列进行同源性和系统进化树分析。结果 4株菌株均被鉴定为奇异变形杆菌;对4株病原菌均高度敏感的药物有丁胺卡那、美罗培南、头孢他啶、头孢噻肟、头孢吡肟、他巴克坦和氨曲南7种,均不敏感的药物有庆大霉素、亚胺培南、头孢唑啉、氨苄西林、克拉维酸、黏菌素、磺胺甲恶唑、氯霉素、环丙沙星和四环素10种;4株菌株的半数致死量(LD50)按BPM-2、BPM-1、SPM、GPM依次降低,鹅源的毒力最高;奇异变形杆菌具有不同的毒力基因谱,8种毒力基因在菌株SPM、GPM、BPM-2中均被检出,菌株BPM-2未检出ucaA基因。BPM-2和BPM-1处于同一分支,但分处于不同的末支;SPM和GPM分处于不同的小分支。结论不同来源的奇异变形杆菌对小鼠的致病力存在较小的差异,且对小鼠有致病力的基因表型与该毒力基因的分布可能存在一定的相关性。展开更多
基金Supported by the National Natural Science Foundation of China(31872530)。
文摘T-2 toxin is the most widespread mycotoxin in crops,feed and food,which poses a serious threat to body health.Bone is the main target tissue for T-2 toxin accumulation.Ingestion of food contaminated by T-2 toxin is the main cause of Kashin-Beck disease.However,the specific mechanism of bone damage caused by T-2 toxin is still unclear.In this study,a total of 40 male C57BL/6N mice were divided into four groups and orally treated with 0,0.5,1.0 and 2.0 mg·kg^(-1) body weight T-2 toxin for 28 days.The results showed that exposure to T-2 toxin led to weight loss,bone mineral density reduction and femoral structural damage of mice.In addition,osteoblast-mediated bone formation was inhibited,and osteoclast-mediated bone resorption was enhanced.Meanwhile,the levels of bone metabolism-related hormones including parathyroid hormone,calcitonin and 1,25-dihydroxyvitamin D3 were reduced.More importantly,it was found that the level of neuropeptide Y(a neurohormone)was decreased.These results provided a new perspetive for understanding the osteotoxicity of T-2 toxin.
文摘目的比较通辽地区不同来源奇异变形杆菌对小鼠致病力及其毒力基因携带情况。方法取4株(SPM、GPM、BPM-1和BPM-2)不同来源的奇异变形杆菌,进行生化鉴定、药物敏感性测试及动物致病性试验,对4株菌株的8种毒力基因(ureC、zapA、mrpA、ucaA、rsbA、pmfA、atfA、atfC)进行PCR检测,对其16S r RNA基因序列进行同源性和系统进化树分析。结果 4株菌株均被鉴定为奇异变形杆菌;对4株病原菌均高度敏感的药物有丁胺卡那、美罗培南、头孢他啶、头孢噻肟、头孢吡肟、他巴克坦和氨曲南7种,均不敏感的药物有庆大霉素、亚胺培南、头孢唑啉、氨苄西林、克拉维酸、黏菌素、磺胺甲恶唑、氯霉素、环丙沙星和四环素10种;4株菌株的半数致死量(LD50)按BPM-2、BPM-1、SPM、GPM依次降低,鹅源的毒力最高;奇异变形杆菌具有不同的毒力基因谱,8种毒力基因在菌株SPM、GPM、BPM-2中均被检出,菌株BPM-2未检出ucaA基因。BPM-2和BPM-1处于同一分支,但分处于不同的末支;SPM和GPM分处于不同的小分支。结论不同来源的奇异变形杆菌对小鼠的致病力存在较小的差异,且对小鼠有致病力的基因表型与该毒力基因的分布可能存在一定的相关性。
