[Objective]The paper was to screen highly efficient fungicides for the prevention and control of tobacco brown spot(Alternaria alternata).[Method]The inhibitory effects of 12 chemical agents against A.alternata were d...[Objective]The paper was to screen highly efficient fungicides for the prevention and control of tobacco brown spot(Alternaria alternata).[Method]The inhibitory effects of 12 chemical agents against A.alternata were determined indoors by mycelium growth rate method.[Result]Toxicity test showed that 30%kresoxim-methyl·boscalid SC had the best inhibitory effect,with an EC_(50)of 1.05μg/mL,followed by 9%pyraclostrobin CS,with an EC_(50)of 2.09μg/mL.As a broad spectrum protective fungicide,77%copper calcium sulfate WP showed a low sensitivity against A.alternata in the petri dish test since it had a mechanism of action different from that of other chemical agents.[Conclusion]The study lays the foundation for subsequent screening of fungicides for the prevention and control of A.alternata in the field.展开更多
为实现对烟草叶片中烟草赤星病菌的SYBR Green I实时定量PCR(q-PCR)快速检测,根据链格孢属ITS基因序列差异位点,设计了1对特异性引物。通过构建含有目的片段的重组质粒,建立了标准曲线,构建了q-PCR快速定量检测方法并对感病叶片进行检测...为实现对烟草叶片中烟草赤星病菌的SYBR Green I实时定量PCR(q-PCR)快速检测,根据链格孢属ITS基因序列差异位点,设计了1对特异性引物。通过构建含有目的片段的重组质粒,建立了标准曲线,构建了q-PCR快速定量检测方法并对感病叶片进行检测,灵敏度达到1.0×10^(-3) ng/μL。实验样品验证表明,该方法可以对烟草赤星病菌进行快速检测和定量。展开更多
The rapid boosting in mobile traffic and the scarcity of available radio spectrum have hindered the improvement of capacity in cellular networks.It is necessary to discover an appropriate coexistence between cellular ...The rapid boosting in mobile traffic and the scarcity of available radio spectrum have hindered the improvement of capacity in cellular networks.It is necessary to discover an appropriate coexistence between cellular and other radio access technologies(mainly Wi-Fi)to offload the high traffic on to unlicensed bands.Dealing with joint time and power allocation to devices,a non-convex problem is modeled to maximize the throughput as well as guarantee the desired user satisfaction.A two-step traffic balancing scheme is proposed to derive the solution.We also focus on the inner competition among cellular users instead of traditional competition between cellular and Wi-Fi in the unlicensed bands.Finally,simulation results show the effectiveness of the proposed two-step traffic balancing scheme.展开更多
基金Supported by Natural Science Foundation of Shandong ProvinceScience and Technology Project of Guizhou,Shaanxi and Sichuan Provincial Company of China National Tobacco Corporation(ZR2018BC037,201910,2019-ZY-1,SXYC-KJ-2017-01,SCYC201703)。
文摘[Objective]The paper was to screen highly efficient fungicides for the prevention and control of tobacco brown spot(Alternaria alternata).[Method]The inhibitory effects of 12 chemical agents against A.alternata were determined indoors by mycelium growth rate method.[Result]Toxicity test showed that 30%kresoxim-methyl·boscalid SC had the best inhibitory effect,with an EC_(50)of 1.05μg/mL,followed by 9%pyraclostrobin CS,with an EC_(50)of 2.09μg/mL.As a broad spectrum protective fungicide,77%copper calcium sulfate WP showed a low sensitivity against A.alternata in the petri dish test since it had a mechanism of action different from that of other chemical agents.[Conclusion]The study lays the foundation for subsequent screening of fungicides for the prevention and control of A.alternata in the field.
文摘为实现对烟草叶片中烟草赤星病菌的SYBR Green I实时定量PCR(q-PCR)快速检测,根据链格孢属ITS基因序列差异位点,设计了1对特异性引物。通过构建含有目的片段的重组质粒,建立了标准曲线,构建了q-PCR快速定量检测方法并对感病叶片进行检测,灵敏度达到1.0×10^(-3) ng/μL。实验样品验证表明,该方法可以对烟草赤星病菌进行快速检测和定量。
基金supported by The National Natural Science Foundation of China(No.61471058)Beijing Nova Program(No.xx2012037).
文摘The rapid boosting in mobile traffic and the scarcity of available radio spectrum have hindered the improvement of capacity in cellular networks.It is necessary to discover an appropriate coexistence between cellular and other radio access technologies(mainly Wi-Fi)to offload the high traffic on to unlicensed bands.Dealing with joint time and power allocation to devices,a non-convex problem is modeled to maximize the throughput as well as guarantee the desired user satisfaction.A two-step traffic balancing scheme is proposed to derive the solution.We also focus on the inner competition among cellular users instead of traditional competition between cellular and Wi-Fi in the unlicensed bands.Finally,simulation results show the effectiveness of the proposed two-step traffic balancing scheme.
