Research Progress of circRNAs during Epithelial- Mesenchymal Transition of Hepatocellular Carcinoma

Hepatocellular carcinoma is prone to invasion and metastasis.It often receives a low diagnosis rate in the early stage but has an extremely high mortality rate.Epithelial-mesenchymal transformation(EMT)is a key factor in promoting tumor cell invasion and metastasis.Circular RNA(circRNA)is involved in regulating EMT in hepatocarcinoma cells through multiple pathways,thereby affecting the occurrence and progression of hepatocellular carcinoma.This article mainly reviews the research progress of circRNA related to EMT core transcription factors,circRNA that promotes EMT in liver cancer,and circRNA that inhibits EMT in liver cancer.

Corrigendum: Research Progress of circRNAs during Epithelial-Mesenchymal Transition of Hepatocellular Carcinoma

Correction The affiliation in the original publication(https://www.doi.org/10.26689/par.v8i2.6010)is incorrect.The original affiliation was:Yuqing Li1,Cuicui Ren1,Yu Cai2,Chang Tian2,Yuanyuan Jia2,Ge Wu1*1Xi’an Medical University,Xi’an 710000,China 2Faculty Development and Teaching Evaluation Office,The First Affiliated Hospital of Xi’an。

Bone Marrow-derived Mesenchymal Stem Cells Promote Microglia/Macrophage M2 Polarization and Enhance Neurogenesis in the Acute and Chronic Stages after Ischemic Stroke

Background:Ischemic stroke has been regarded as a major cause of disability and death around the world due to limited effective therapies.Accumulating evidence have shown that although microglia are polarized to an anti-inflammatory M2 phenotype in the early stage of ischemia,they transform progressively into a proinflammatory M1 phenotype.Bone marrow-derived mesenchymal stem cells(BMSCs)may be used to treat ischemic injury through regulating the poststroke inflammatory response.However,the mechanism by which BMSCs can treat ischemic stroke remains unclarified.Objective:This study aimed to investigate whether BMSCs shift M1-to-M2 phenotype transformation of mi-croglia/macrophages and enhance neurogenesis in a rat transient middle cerebral artery occlusion(tMCAO)model.Methods:Ninety-minute tMCAO was applied to the rats,followed by reperfusion.BMSCs were transplanted into the rats via intravenous injection at 24 h after tMCAO.After being randomly divided into the sham group,the MCAO group,and the BMSCs group,the rats’behavior was assessed at 1,3,7,and 14 days following tM-CAO.qRT-PCR,double-immunofluorescence staining,and Western blot were performed at 3 and 14 days after tMCAO to determine M1/M2 polarization of microglia/macrophages.Neurogenesis was examined by double-immunofluorescence staining at 14 days after tMCAO.Expression of brain-derived neurotrophic factor(BDNF)was measured on the protein level by immunofluorescence staining at 3 and 14 days after tMCAO.Results:We found that BMSCs treatment promoted the recovery of neurological function after tMCAO,inhibited the expression of TNF𝛼,iNOS and CD16/32,which are markers of M1 microglia/macrophage,and enhanced the expression of IL10,TGF𝛽and CD206 that are markers of M2 microglia/macrophage.Moreover,BMSCs treatment promoted neurogenesis and M2-derived BDNF expression after tMCAO.Conclusion:It is indicated by the results that BMSCs modulate neuroinflammation and enhance neurogenesis,which could be due to transforming microglia/macrophages from the M1 polarization state towards M2 in a rat tMCAO model.