Gene targeting is a powerful approach of studying the gene function in vivo. Specific genetic modifications, including simple gene disruption, point mutations, large chromosomal deletions and rearrangements, targeted ...Gene targeting is a powerful approach of studying the gene function in vivo. Specific genetic modifications, including simple gene disruption, point mutations, large chromosomal deletions and rearrangements, targeted incorporation of foreign genes, could be introduced into the mouse genome by gene targeting. Recent studies make it possible to do the gene targeting with temporal and spatial control.展开更多
The human telomerase reverse transcriptase （hTERT） is expressed in more than 85% of tumor cells but is usually not found in normal cells, which makes hTERT as an ideal tumor-associate antigen （TAA） to develop pote...The human telomerase reverse transcriptase （hTERT） is expressed in more than 85% of tumor cells but is usually not found in normal cells, which makes hTERT as an ideal tumor-associate antigen （TAA） to develop potential vaccine specifically destroying cancers without impairing normal tissues in human cancer immunotherapy. Here are reviewed the fundamental advances of studies on immunogenicity of hTERT or its peptides and the early clinical trials using the hTERT vaccine approach in the last decades.展开更多
Conotoxins are short peptide-toxins with specific targets and large diversity. They are usetul in analgesia, neuroprotection, detection of some kinds of deseases, and receptor and ion channel study. in order to explor...Conotoxins are short peptide-toxins with specific targets and large diversity. They are usetul in analgesia, neuroprotection, detection of some kinds of deseases, and receptor and ion channel study. in order to explore the conotoxin resourses of Chinese oceans, rapid amplification of 3’ cDNA ends (RACE) method was utilized to systemically analyze the O-superfamily conotoxin content of Conus striatus inhabited near Chinese Hainan Island. Six new O-superfamily conopeptides were identified, one of which is highly homologous to MVIlA, an N-type calcium channel antagonist.展开更多
基金the National Natural Science Foundation of China (Grant Nos. 39970413 and 30070837) the National High Technology Research and Development Program (Grant No. 102-08-08-02) Beijing Science Projects (Grant No. 954020600) and the Innovation Initiation Fu
文摘Gene targeting is a powerful approach of studying the gene function in vivo. Specific genetic modifications, including simple gene disruption, point mutations, large chromosomal deletions and rearrangements, targeted incorporation of foreign genes, could be introduced into the mouse genome by gene targeting. Recent studies make it possible to do the gene targeting with temporal and spatial control.
文摘The human telomerase reverse transcriptase （hTERT） is expressed in more than 85% of tumor cells but is usually not found in normal cells, which makes hTERT as an ideal tumor-associate antigen （TAA） to develop potential vaccine specifically destroying cancers without impairing normal tissues in human cancer immunotherapy. Here are reviewed the fundamental advances of studies on immunogenicity of hTERT or its peptides and the early clinical trials using the hTERT vaccine approach in the last decades.
文摘Conotoxins are short peptide-toxins with specific targets and large diversity. They are usetul in analgesia, neuroprotection, detection of some kinds of deseases, and receptor and ion channel study. in order to explore the conotoxin resourses of Chinese oceans, rapid amplification of 3’ cDNA ends (RACE) method was utilized to systemically analyze the O-superfamily conotoxin content of Conus striatus inhabited near Chinese Hainan Island. Six new O-superfamily conopeptides were identified, one of which is highly homologous to MVIlA, an N-type calcium channel antagonist.
