Objective:To investigate plasmid DNA profiles and the antibiotic resistance of a total of 41 strains of Escherichia coli(E.coli)isolated from seawater and mussel collected from 15 different sampling stations in Sinop,...Objective:To investigate plasmid DNA profiles and the antibiotic resistance of a total of 41 strains of Escherichia coli(E.coli)isolated from seawater and mussel collected from 15 different sampling stations in Sinop,Turkey.Methods:Most probable number technique was used for detection of E.coli.Antibiotic susceptibilities of the isolates were determined by the disc diffusion method.Plasmid DNA of the strains was extracted by the alkaline lyses procedure.Results:According to morphological and physiological properties,it was determined that the isolates belonged to E.coli species.Antibiotic susceptibility of the strains was determined against seven standard drugs using disc diffusion method.All isolates were resistant to bacitracin(100%),novobiocin(100%),ampicillin(12.5%),tetracycline(7.5%),ceftazidime(5%)and imipenem(2.5%),respectively,whereas the strains were susceptible to polymyxin B(100%).The multiple antibiotic resistance values for the strains were found in range from 0.28 to 0.57.In addition,plasmid DNA analyses results confirmed that 22 strains harbored a single or more than two plasmids sized approximately between 24.500 to 1.618 bp.The high-size plasmid(14.700 bp)was observed as common in 21 of all strains.Conclusions:As a result,our study indicated that the presence of antibiotic resistant E.coli strains in seawater and mussel might be potential risk for public health issue.展开更多
文摘Objective:To investigate plasmid DNA profiles and the antibiotic resistance of a total of 41 strains of Escherichia coli(E.coli)isolated from seawater and mussel collected from 15 different sampling stations in Sinop,Turkey.Methods:Most probable number technique was used for detection of E.coli.Antibiotic susceptibilities of the isolates were determined by the disc diffusion method.Plasmid DNA of the strains was extracted by the alkaline lyses procedure.Results:According to morphological and physiological properties,it was determined that the isolates belonged to E.coli species.Antibiotic susceptibility of the strains was determined against seven standard drugs using disc diffusion method.All isolates were resistant to bacitracin(100%),novobiocin(100%),ampicillin(12.5%),tetracycline(7.5%),ceftazidime(5%)and imipenem(2.5%),respectively,whereas the strains were susceptible to polymyxin B(100%).The multiple antibiotic resistance values for the strains were found in range from 0.28 to 0.57.In addition,plasmid DNA analyses results confirmed that 22 strains harbored a single or more than two plasmids sized approximately between 24.500 to 1.618 bp.The high-size plasmid(14.700 bp)was observed as common in 21 of all strains.Conclusions:As a result,our study indicated that the presence of antibiotic resistant E.coli strains in seawater and mussel might be potential risk for public health issue.
