Ultraviolet light A irradiation induces immunosuppression associated with the generation of reactive oxygen species in human neutrophils

Ultraviolet blood irradiation has been used as a physical therapy to treat many nonspeci¯c diseases in clinics;however,the underlying mechanisms remain largely unclear.Neutrophils,the first line of host defense,play a crucial role in a variety of in°ammatory responses.In the present work,we investigated the effects of ultraviolet light A(UVA)on the immune functions of human neutrophils at the single-cell level by using an inverted°uorescence microscope.N-Formylmethionyl-leucyl-phenylalanine(FMLP),a classic physiological chemotactic peptide,was used to induce a series of immune responses in neutrophils in vitro.FMLP-induced calcium mobilization,migration,and phagocytosis in human neutrophils was significantly blocked after treatment with 365 nm UVA irradiation,demonstrating the immunosuppressive effects of UVA irradiation on neutrophils.Similar responses were also observed when the cells were pretreated with H2O2,a type of reactive oxygen species(ROS).Furthermore,UVA irradiation resulted in an increase in NAD(P)H,a member of host oxidative stress in cells.Taken together,our data indicate that UVA irradiation results in immunosuppression associated with the production of ROS in human neutrophils.

Multimodal collaborative BCI system based on the improved CSP feature extraction algorithm

Background As a novel approach for people to directly communicate with an external device,the study of brain-computer interfaces(BCIs)has become well-rounded.However,similar to the real-world scenario,where individuals are expected to work in groups,the BCI systems should be able to replicate group attributes.Methods We proposed a 4-order cumulants feature extraction method(CUM4-CSP)based on the common spatial patterns(CSP)algorithm.Simulation experiments conducted using motion visual evoked potentials(mVEP)EEG data verified the robustness of the proposed algorithm.In addition,to freely choose paradigms,we adopted the mVEP and steady-state visual evoked potential(SSVEP)paradigms and designed a multimodal collaborative BCI system based on the proposed CUM4-CSP algorithm.The feasibility of the proposed multimodal collaborative system framework was demonstrated using a multiplayer game controlling system that simultaneously facilitates the coordination and competitive control of two users on external devices.To verify the robustness of the proposed scheme,we recruited 30 subjects to conduct online game control experiments,and the results were statistically analyzed.Results The simulation results prove that the proposed CUM4-CSP algorithm has good noise immunity.The online experimental results indicate that the subjects could reliably perform the game confrontation operation with the selected BCI paradigm.Conclusions The proposed CUM4-CSP algorithm can effectively extract features from EEG data in a noisy environment.Additionally,the proposed scheme may provide a new solution for EEG-based group BCI research.

The Decision Strategies of Adolescents with Different Emotional Stabilities in Unfair Situations

Dear Editor,Neuroticism,a crucial dimension of human personality,fluctuates from emotional stability to instability[1].Compared to those with emotional stability,individuals with emotional instability show stronger attentional bias to negative stimuli,lower life satisfaction,and tend to perform poorly(i.e.,fewer correct responses)in cognitive tasks.For instance,individuals with different emotional stabilities experience distinct decision-making responses,especially when facing inequity,as the unfairness induces typical conflict between self-interest and fairness[2].