The development of neural tissue engineering has brought new hope to the treatment of spinal cord injury(SCI).Up to date,various scaffolds have been developed to induce the oriented growth and arrangement of nerves to...The development of neural tissue engineering has brought new hope to the treatment of spinal cord injury(SCI).Up to date,various scaffolds have been developed to induce the oriented growth and arrangement of nerves to facilitate the repair after injury.In this work,a conductive and anisotropic inverse opal substrate was presented by modifying polystyrene(PS)inverse opal films with carbon nanotubes and then stretching them to varying degrees.The film had good biocompatibility,and neural stem cells(NSCs)grown on the film displayed good orientation along the stretching direction.In addition,benefiting from the conductivity and anisotropy of the film,NSCs differentiated into neurons significantly.These results suggest that the conductive and anisotropic PS inverse opal substrates possess value in nerve tissue engineering regeneration.展开更多
基金This work was supported by grants from National Key R&D Program of China(Nos.2021YFA1101300,2021YFA1101800,2020YFA0112503)Strategic Priority Research Program of the Chinese Academy of Science(No.XDA16010303)+4 种基金National Natural Science Foundation of China(Nos.82030029,81970882,92149304,82201292)Science and Technology Department of Sichuan Province(No.2021YFS0371)Shenzhen Fundamental Research Program(Nos.JCYJ20190814093401920,JCYJ20210324125608022)Open Research Fund of State Key Laboratory of Genetic Engineering,Fudan University(No.SKLGE-2104)Open Research Fund of Guangdong Academy of Medical Sciences(YKY-KF202201).
文摘The development of neural tissue engineering has brought new hope to the treatment of spinal cord injury(SCI).Up to date,various scaffolds have been developed to induce the oriented growth and arrangement of nerves to facilitate the repair after injury.In this work,a conductive and anisotropic inverse opal substrate was presented by modifying polystyrene(PS)inverse opal films with carbon nanotubes and then stretching them to varying degrees.The film had good biocompatibility,and neural stem cells(NSCs)grown on the film displayed good orientation along the stretching direction.In addition,benefiting from the conductivity and anisotropy of the film,NSCs differentiated into neurons significantly.These results suggest that the conductive and anisotropic PS inverse opal substrates possess value in nerve tissue engineering regeneration.
