Two-line hybrid rice with excellent quality is preferred in the Chinese market.However,there is a trade-off between reducing costs for hybrid seed production and lowering the outcrossing rate of the sterile line,which...Two-line hybrid rice with excellent quality is preferred in the Chinese market.However,there is a trade-off between reducing costs for hybrid seed production and lowering the outcrossing rate of the sterile line,which is largely determined by the stigma exsertion rate(SER).In this study,we constructed mutants of male sterility lines with improved grain length(GL)and SER in three elite early-season indica rice varieties through targeted manipulation of the TMS5 and GS3 genes using CRISPR/Cas9-mediated multiplex systems.We obtained a series of marker-free gs3 single mutants and gs3tms5 double mutants with significantly higher SER,longer grains,and increased 1000-grain weight compared with the wild type(WT).Importantly,the typically thermo-sensitive genic male sterile(TGMS)trait with a higher SER was observed in gs3tms5 mutants,and their F1 hybrids exhibited remarkable improvements in grain shape and yield-related traits.Our findings provided an efficient method to generate new valuable TGMS germplasm with improved SER through the mutagenesis of GS3 and TMS5 synergistically,and demonstrated that GS3 had pleiotropic effects on grain size,SER,and grain quality in early-season indica rice.展开更多
目的探讨椎体骨质疏松性骨折椎体成形术后骨水泥弥散分布与疗效的关系。方法选择2020年1月至2022年4月在本院行椎体成形术的80例椎体骨质疏松性骨折患者作为研究对象。根据术后骨水泥在伤椎椎体中的弥散体积比,结合骨水泥在正侧位片上...目的探讨椎体骨质疏松性骨折椎体成形术后骨水泥弥散分布与疗效的关系。方法选择2020年1月至2022年4月在本院行椎体成形术的80例椎体骨质疏松性骨折患者作为研究对象。根据术后骨水泥在伤椎椎体中的弥散体积比,结合骨水泥在正侧位片上的弥散状态,分为1级组(<25%,n=36),2级组(25%~50%,n=24)和3级组(50%~75%,n=20)。比较三组患者术前、术后的Oswestry功能障碍指数(Oswestry disability index,ODI)、伤椎后凸角、伤椎前柱高度变化、术前、术后疼痛视觉模拟评分(visual analog scale,VAS)和并发症发生率。结果三组术后、术后1个月、术后3个月、术后6个月的VAS、ODI均有所改善(P<0.05);三组术后、术后6个月伤椎椎体后凸角均比术前减小(P<0.05),且2级组和3级组术后、术后6个月小于同期1级组(P<0.05),但2级组和3级组比较无统计学差异(P>0.05);三组术后、术后6个月椎体前柱均比术前增高(P<0.05),且2级组和3级组术后、术后6个月椎体前柱均高于1级组(P<0.05),但2级组和3级组比较无统计学差异(P>0.05)。结论椎体成形术后骨水泥的弥散分布会影响术后影像学指标,但弥散体积比达到50%即可获得较为理想的疗效。展开更多
基金the Natural Science Foundation of Zhejiang Province,China(Grant Nos.LY24C130004,LY22C135104,and LY23C130002)the National Natural Science Foundation of China(Grant No.31501288)+1 种基金the Open Project Program of State Key Laboratory of Rice Biology and Breeding,China(Grant No.20210207)Central Publicinterest Scientific Institution Basal Research Fund,China(Grant No.CPSIBRF-CNRRI-202203).
文摘Two-line hybrid rice with excellent quality is preferred in the Chinese market.However,there is a trade-off between reducing costs for hybrid seed production and lowering the outcrossing rate of the sterile line,which is largely determined by the stigma exsertion rate(SER).In this study,we constructed mutants of male sterility lines with improved grain length(GL)and SER in three elite early-season indica rice varieties through targeted manipulation of the TMS5 and GS3 genes using CRISPR/Cas9-mediated multiplex systems.We obtained a series of marker-free gs3 single mutants and gs3tms5 double mutants with significantly higher SER,longer grains,and increased 1000-grain weight compared with the wild type(WT).Importantly,the typically thermo-sensitive genic male sterile(TGMS)trait with a higher SER was observed in gs3tms5 mutants,and their F1 hybrids exhibited remarkable improvements in grain shape and yield-related traits.Our findings provided an efficient method to generate new valuable TGMS germplasm with improved SER through the mutagenesis of GS3 and TMS5 synergistically,and demonstrated that GS3 had pleiotropic effects on grain size,SER,and grain quality in early-season indica rice.
文摘目的探讨椎体骨质疏松性骨折椎体成形术后骨水泥弥散分布与疗效的关系。方法选择2020年1月至2022年4月在本院行椎体成形术的80例椎体骨质疏松性骨折患者作为研究对象。根据术后骨水泥在伤椎椎体中的弥散体积比,结合骨水泥在正侧位片上的弥散状态,分为1级组(<25%,n=36),2级组(25%~50%,n=24)和3级组(50%~75%,n=20)。比较三组患者术前、术后的Oswestry功能障碍指数(Oswestry disability index,ODI)、伤椎后凸角、伤椎前柱高度变化、术前、术后疼痛视觉模拟评分(visual analog scale,VAS)和并发症发生率。结果三组术后、术后1个月、术后3个月、术后6个月的VAS、ODI均有所改善(P<0.05);三组术后、术后6个月伤椎椎体后凸角均比术前减小(P<0.05),且2级组和3级组术后、术后6个月小于同期1级组(P<0.05),但2级组和3级组比较无统计学差异(P>0.05);三组术后、术后6个月椎体前柱均比术前增高(P<0.05),且2级组和3级组术后、术后6个月椎体前柱均高于1级组(P<0.05),但2级组和3级组比较无统计学差异(P>0.05)。结论椎体成形术后骨水泥的弥散分布会影响术后影像学指标,但弥散体积比达到50%即可获得较为理想的疗效。
