Nerve conduit is one of strategies for spine cord injury(SCI)treatment.Recently,studies showed that biomaterials could guide the neurite growth and promote axon regeneration at the injury site.However,the scaffold by ...Nerve conduit is one of strategies for spine cord injury(SCI)treatment.Recently,studies showed that biomaterials could guide the neurite growth and promote axon regeneration at the injury site.However,the scaffold by itself was difficult to meet the need of SCI functional recovery.The basic fibroblast growth factor(bFGF)administration significantly promotes functional recovery after organ injuries.Here,using a rat model of T9 hemisected SCI,we aimed at assessing the repair capacity of implantation of collagen scaffold(CS)modified by collagen binding bFGF(CBD-bFGF).The results showed that CS combined with CBD-bFGF treatment improved survival rates after the lateral hemisection SCI.The CS/CBD-bFGF group showed more significant improvements in motor than the simply CS-implanted and untreated control group,when evaluated by the 21-point Basso-Beattie-Bresnahan(BBB)score and footprint analysis.Both hematoxylin and eosin(H&E)and immunohistochemical staining of neurofilament(NF)and glial fibrillary acidic protein(GFAP)demonstrated that fibers were guided to grow through the implants.These findings indicated that administration of CS modified with CBD-bFGF could promote spinal cord regeneration and functional recovery.展开更多
文摘为提高红托竹荪干燥品质并获得最佳干燥工艺,采用真空红外干燥(vacuum infrared drying,VID)、气流冲击干燥(air impingement drying,AID)、控湿干燥(moisture control drying,MCD)等不同干燥方式对红托竹荪进行对比研究,以热风干燥(hot air drying,HAD)作为对照组,研究不同干燥方式及温度对红托竹荪干燥品质的影响。试验结果表明不同干燥方式对竹荪宏观品质产生了显著影响,其中MCD可获得最小的色差∆E和收缩率,AID则能够保证较高的复水比;干燥速率方面,MCD在前期能够获得较高的干燥速率,但后期干燥速率会放缓,而AID在整个干燥过程都具有较高的干燥速率,干燥时间较短;在成分保留上,MCD可以保留较高含量的多糖、三萜和黄酮,而采用VID可以有效保护多酚。单位能耗随干燥温度的升高明显降低,不同方式下VID的干燥能耗值整体偏大,MCD的单位能耗最低(18.82 kW·h/kg)。通过主成分分析法,上述干燥方式对红托竹荪综合评分后得到的结果排序为:MCD>AID>VID>HAD,MCD干燥方式中采用60℃相对湿度为40%,保湿时间15min的干燥工艺综合品质较佳,在此加工方式下的红托竹荪综合评分为2.94,该研究可为红托竹荪高品质干燥提供一定参考。
基金supported by National Natural Science Foundation of China(81101369,81071450)the Scientific Research Foundation for the Returned Overseas Chinese Scholars,Ministry of Education of China(to Shi Qin),Ph.D.Programs Foundation of State Education Ministry(20113201110013)+1 种基金Jiangsu Provincial Special Program of Medical Science(BL2012004,BK2011264)Jiangsu Province’s Key Provincial Talents Program(RC2011102)
文摘Nerve conduit is one of strategies for spine cord injury(SCI)treatment.Recently,studies showed that biomaterials could guide the neurite growth and promote axon regeneration at the injury site.However,the scaffold by itself was difficult to meet the need of SCI functional recovery.The basic fibroblast growth factor(bFGF)administration significantly promotes functional recovery after organ injuries.Here,using a rat model of T9 hemisected SCI,we aimed at assessing the repair capacity of implantation of collagen scaffold(CS)modified by collagen binding bFGF(CBD-bFGF).The results showed that CS combined with CBD-bFGF treatment improved survival rates after the lateral hemisection SCI.The CS/CBD-bFGF group showed more significant improvements in motor than the simply CS-implanted and untreated control group,when evaluated by the 21-point Basso-Beattie-Bresnahan(BBB)score and footprint analysis.Both hematoxylin and eosin(H&E)and immunohistochemical staining of neurofilament(NF)and glial fibrillary acidic protein(GFAP)demonstrated that fibers were guided to grow through the implants.These findings indicated that administration of CS modified with CBD-bFGF could promote spinal cord regeneration and functional recovery.