目的收集沧州市水产品中副溶血性弧菌的毒力基因和脉冲场凝胶电泳(pulsed field gel electrophoresis,PFGE)分型特征,为副溶血性弧菌的防控提供参考。方法对2020—2022年1056份海产品样本中分离出的139株副溶血性弧菌进行三重实时荧光...目的收集沧州市水产品中副溶血性弧菌的毒力基因和脉冲场凝胶电泳(pulsed field gel electrophoresis,PFGE)分型特征,为副溶血性弧菌的防控提供参考。方法对2020—2022年1056份海产品样本中分离出的139株副溶血性弧菌进行三重实时荧光聚合酶链式反应(polymerase chain reaction,PCR)技术检测本地区副溶血性弧菌的毒力基因;通过脉冲场凝胶电泳的基因图谱得到聚类图,采用BioNumerics 7.6软件对PFGE酶切片段聚类分析。分类变量采用卡方检验进行比较,双侧检验,以P<0.05为差异有统计学意义。结果1056份样本检出139份副溶血弧菌阳性标本,检出率为13.16%,其中139株中有3株热稳定直接溶血素(thermostable direct hemolysin,tdh)基因阳性,具有毒力,带有毒力基因的副溶血性弧菌占比为2.16%。139株副溶血性弧菌部分同源,聚类分析相似度为36.5%~100.0%。结论沧州地区带毒力的副溶血性弧菌主要为tdh毒力型,毒力基因分布特征与其他地区报道基本一致;PFGE图谱多态化,可有效应用于食源性疾病溯源分析及分子流行病学研究。为开展本地区副溶血性弧菌分布研究,预防副溶血性弧菌相关疾病的暴发打下基础。展开更多
Objective:To observe the clinical efficacy of moxibustion at Baihui(GV20)plus Tuina(Chinese therapeutic massage)for children with postnasal drip syndrome(PNDS).Methods:A total of 60 cases were divided into an observat...Objective:To observe the clinical efficacy of moxibustion at Baihui(GV20)plus Tuina(Chinese therapeutic massage)for children with postnasal drip syndrome(PNDS).Methods:A total of 60 cases were divided into an observation group and a control group according to the random number table method,with 30 cases in each group.Cases in the control group received mometasone furoate nasal spray and nasal irrigation with 0.9%normal saline.Cases in the observation group received additional moxibustion at Baihui(GV20)plus Tuina at the head and face.All the cases were treated for four consecutive weeks.The clinical efficacy and traditional Chinese medicine(TCM)symptoms score were compared between the two groups.Results:The total effective rate of the observation group was 93.3%while that of the control group was 70.0%.The difference between the two groups was statistically significant(P<0.05).After treatment,the TCM symptom scores in both groups declined significantly,showing a statistical difference from that before treatment(P<0.05),and the score of the observation group was notably lower than that of the control group(P<0.05).Conclusion:The clinical efficacy of moxibustion at Baihui(GV20)plus Tuina at the head and face on the basis of nasal spray with Western medicine is more significant than that of Western medicine alone for PNDS in children.展开更多
文摘目的收集沧州市水产品中副溶血性弧菌的毒力基因和脉冲场凝胶电泳(pulsed field gel electrophoresis,PFGE)分型特征,为副溶血性弧菌的防控提供参考。方法对2020—2022年1056份海产品样本中分离出的139株副溶血性弧菌进行三重实时荧光聚合酶链式反应(polymerase chain reaction,PCR)技术检测本地区副溶血性弧菌的毒力基因;通过脉冲场凝胶电泳的基因图谱得到聚类图,采用BioNumerics 7.6软件对PFGE酶切片段聚类分析。分类变量采用卡方检验进行比较,双侧检验,以P<0.05为差异有统计学意义。结果1056份样本检出139份副溶血弧菌阳性标本,检出率为13.16%,其中139株中有3株热稳定直接溶血素(thermostable direct hemolysin,tdh)基因阳性,具有毒力,带有毒力基因的副溶血性弧菌占比为2.16%。139株副溶血性弧菌部分同源,聚类分析相似度为36.5%~100.0%。结论沧州地区带毒力的副溶血性弧菌主要为tdh毒力型,毒力基因分布特征与其他地区报道基本一致;PFGE图谱多态化,可有效应用于食源性疾病溯源分析及分子流行病学研究。为开展本地区副溶血性弧菌分布研究,预防副溶血性弧菌相关疾病的暴发打下基础。
文摘Objective:To observe the clinical efficacy of moxibustion at Baihui(GV20)plus Tuina(Chinese therapeutic massage)for children with postnasal drip syndrome(PNDS).Methods:A total of 60 cases were divided into an observation group and a control group according to the random number table method,with 30 cases in each group.Cases in the control group received mometasone furoate nasal spray and nasal irrigation with 0.9%normal saline.Cases in the observation group received additional moxibustion at Baihui(GV20)plus Tuina at the head and face.All the cases were treated for four consecutive weeks.The clinical efficacy and traditional Chinese medicine(TCM)symptoms score were compared between the two groups.Results:The total effective rate of the observation group was 93.3%while that of the control group was 70.0%.The difference between the two groups was statistically significant(P<0.05).After treatment,the TCM symptom scores in both groups declined significantly,showing a statistical difference from that before treatment(P<0.05),and the score of the observation group was notably lower than that of the control group(P<0.05).Conclusion:The clinical efficacy of moxibustion at Baihui(GV20)plus Tuina at the head and face on the basis of nasal spray with Western medicine is more significant than that of Western medicine alone for PNDS in children.
