Impact of Water-Sediment Regulation on Variations of Amino Acids in the Middle-Lower Yellow River, China

In order to examine the impacts of water-sediment regulation on regional carbon cycling,we collected water,particulate and sediment samples from the middle-lower Yellow River in late June and early July,2015 and analyzed their specific amino acids(AA),DOC,POC,and bacteria abundance.Summarized by 14 specific AA,the total hydrolysable AA(THAA),particulate AA(PAA),and sediment AA(SAA)varied in ranges of 2.29-9.05μmol L^-1,5.22-22.96μmol L^-1,and 81.7-137.19μg g^-1 dry weight.After the regulation,dissolved free AA(DFAA)decreased by 29%while DCAA increased by 72%.These variations suggested that DFAA were further degraded,while DCAA molecules were further activated.Meanwhile,PAA increased almost 4 times as many as those before regulation,and SAA increased as well.After regulation,the amounts of bioactive amino acids(Asp,Glu and Gly)increased in THAA but decreased in PAA,with little changes in SAA.The ratios of Asp/Gly in different phases increased after regulation,indicating the AA contributions were promoted by calcareous organisms rather than by siliceous organisms.Multiple correlation analysis showed that PAA was primary representatives of AA and organic carbon,followed by DCAA and POC.Moreover,bacterial reproduction played a key role in shaping the AA compositions and properties,followed by the redox condition and acid-base balance.The results of this study provided a clear evidence for the effects of water-sediment regulation on regional biogeochemistry of organic carbon in the middle-lower Yellow River.

腹腔镜胆囊切除术联合胆道镜治疗胆囊结石合并胆总管结石的临床分析

目的对胆囊结石合并胆总管结石患者在采用腹腔镜胆囊切除术联合胆道镜治疗后的临床效果进行分析。方法便利选取2019年1月—2020年10月该院收治的100例患者进行研究,分组依据为治疗方案的差异性,分为对照组与观察组,每组50例。对照组患者采用开腹胆囊切除术联合T管引流术与胆总管切开探查的方案;观察组患者采用腹腔镜胆囊切除术联合胆道镜取石术的方案进行治疗。对两组患者的治疗效果进行比较。结果治疗后,观察组手术时间(134.23±18.50)min,对照组手术时间(132.17±25.20)min,两组比较,差异无统计学意义(P>0.05)。治疗后,观察组住院时间(12.20±4.10)d,对照组住院时间(15.30±2.20)d,差异有统计学意义(P<0.05)。两组患者经过不同方案进行治疗后,对照组患者的总满意度仅为84%,低于观察组患者的总满意度96%,差异有统计学意义(χ^(2)=4.000,P<0.05)。结论在当前的临床治疗中,针对胆囊结石合并胆总管结石患者的微创手术治疗方案,可以选择腹腔镜胆囊切除术联合胆道镜取石术进行治疗,减少患者手术时间与住院时间改善患者治疗效果。

辽河口芦苇湿地积水营养盐组成和结构的周日变化特征

本文于2015年5、6和9月对辽河口芦苇湿地积水进行营养盐昼夜变化监测,分析了其不同形态氮、磷组成和时空变化特征,研究了温度、盐度、pH、DO和叶绿素a理化因子,与营养盐组成和结构的关系。结果表明,芦苇湿地积水中总溶解态氮(TDN)昼夜变化相对稳定,芦苇生长初期、快速生长期和成熟期TDN含量范围依次为1.35-3.12mg/L(平均为2.07mg/L)、0.82-3.06mg/L(平均1.31mg/L)和0.31-12.59mg/L(平均3.26mg/L);虽然TDN的组成结构不断变化,但是DON始终是TN的主要组分,约占81-99%。总溶解磷(TDP)含量范围依次为0.04-0.12mg/L(平均为0.07mg/L)、0.03-0.12mg/L(平均0.06mg/L)和0.02-0.13mg/L(平均0.07mg/L)。芦苇生长期内水体中溶解性氮磷比(N/P)除5月4点外,均小于12,芦苇快速生产期(1.28)和成熟期(2.40)积水中的N/P比平均水平低于芦苇生长初期(5.47),积水中氮处于相对匮乏的状态,可能与芦苇对氮的大量吸收相关。温度、盐度、pH、DO和叶绿素a与不同形态营养盐的相关分析结果表明,芦苇湿地系统不仅存在旺盛的芦苇生产活动,也存在较强的微藻初级生产和微生物再生产活动,环境因子通过影响芦苇和积水微藻的初级生产,以及积水中微生物的再生产活动,显著影响营养盐组成和结构的时空变化。