目的运用欧洲药学监护联盟(Pharmaceutical Care Network Europe,PCNE)分类系统识别并分类骨科住院患者的药物相关问题(drug-related problems,DRPs),以促进骨科患者合理用药。方法分析2021年1月1日~2021年2月28日我院骨科住院患者的病...目的运用欧洲药学监护联盟(Pharmaceutical Care Network Europe,PCNE)分类系统识别并分类骨科住院患者的药物相关问题(drug-related problems,DRPs),以促进骨科患者合理用药。方法分析2021年1月1日~2021年2月28日我院骨科住院患者的病历及医嘱信息,基于PCNE分类系统对DRPs进行分析。结果共纳入209名骨科住院患者,识别出253个DRPs问题,平均DRPs发生率为1.21次/人。主要为治疗安全性问题,共192个(75.89%),其次为61个(24.11%)治疗有效性问题。DRPs原因分析中最主要的为剂型选择问题,共182例次(71.93),其次有36例次(14.23%)为药物选择问题。结论通过PCNE分类系统发现我院DRPs发生率较高,药师需针对剂型选择和药物选择等问题开展相应的药学服务,以促进骨科患者合理用药。展开更多
目的:探讨iDose4迭代重建技术在学龄前儿童胸部CT低剂量平扫中的应用研究。方法:连续选取150例行胸部CT平扫2~6岁患儿,随机分为常规剂量组(50 mAs)、低剂量25 mAs组和低剂量10 mAs组。常规剂量组采用FBP重建作为常规对照组,低剂量扫描...目的:探讨iDose4迭代重建技术在学龄前儿童胸部CT低剂量平扫中的应用研究。方法:连续选取150例行胸部CT平扫2~6岁患儿,随机分为常规剂量组(50 mAs)、低剂量25 mAs组和低剂量10 mAs组。常规剂量组采用FBP重建作为常规对照组,低剂量扫描组分别采用FBP及iDose4-5对图像进行重建。2名放射科副主任医师对图像主观质量行盲法阅片,比较不同毫安秒和重建算法组图像主客观质量及辐射剂量并行统计学分析。结果:25 mAs iDose4组较25 mAs FBP组图像主客观质量增高,10 mAs iDose4组较10 mAs滤波反投影(FBP)组图像主客观质量增高。与常规剂量组比较,25 mAs FBP组图像主客观质量降低,25 mAs iDose4组图像主客观质量增高,10 mAs FBP组图像主客观质量降低,10 mAs iDose4组图像主客观质量差异无统计学意义。低剂量25 mAs、10 mAs组有效剂量较常规剂量组分别降低52.6%和78.2%。结论:行学龄前儿童胸部CT低剂量平扫时,采用25 mAs、10 mAs联合iDose4-5技术,能在降低辐射剂量的同时,获得与常规剂量更优或相当的图像质量。展开更多
The aim of the study was to establish a set of differential strains and to identify soybean resistant genes to Phytophthora root rot and then to apply those strains for analysis of the resistant genes Rps1a,Rps1c,and ...The aim of the study was to establish a set of differential strains and to identify soybean resistant genes to Phytophthora root rot and then to apply those strains for analysis of the resistant genes Rps1a,Rps1c,and Rps1k that soybean cultivars or lines may carry.Virulence formula of 125 Phytophthora sojae isolates were determined using the hypocotyls inoculation technique,the strains,which includ 6 isolates with different virulence formulas,were applied to identify the resistance of 55 soybean cultivars or lines and resistant genes were analyzed using the gene postulating procedure.Eighteen reaction types occurred in 55 cultivars or lines and results of gene postulation indicated that 2 cultivars or lines probably carried gene Rps1c and no cultivar may carry genes Rps1a or Rps1k.A few of soybean cultivars or lines from Huanghuai Region carry Rps genes Rps1a,Rps1c and Rps1k and tend to infect by P.sojae,so resistant cultivars or lines need to be bred and popularized actively.展开更多
Foot and root rot caused by Phytophthora parasitica is a substantial threat to citrus cultivation,affecting both yield and quality.Thus,rapid and accurate detection of P.parasitica plays an important role in disease m...Foot and root rot caused by Phytophthora parasitica is a substantial threat to citrus cultivation,affecting both yield and quality.Thus,rapid and accurate detection of P.parasitica plays an important role in disease management.The aim of this study was to develop a simple diagnostic method to detect P.parasitica infection by combining recombinase polymerase amplification and lateral flow strips(LF-RPA).To establish the LF-RPA assay of P.parasitica,the primers and probe designed based on the Ypt1 gene were tested for specificity to P.parasitica,which showed no cross-reactivity with DNAs of other related oomycete species.The LF-RPA assay detected the amount of genomic DNA of P.parasitica which was as low as 1 pg.To make the LF-RPA assay useful in low-resource settings,four simplified DNA extraction methods were compared,after which the LF-RPA assay was applied,with no specialized equipment,to analyze a diverse range of citrus tissues by using a simplified PEG-NaOH method for DNA extraction.This method was successful in detecting P.parasitica in infected plant samples within 30 min.Combining the LF-RPA assay and a simplified DNA extraction method could be a potential detection test for P.parasitica,especially in areas with limited resources.展开更多
文摘目的运用欧洲药学监护联盟(Pharmaceutical Care Network Europe,PCNE)分类系统识别并分类骨科住院患者的药物相关问题(drug-related problems,DRPs),以促进骨科患者合理用药。方法分析2021年1月1日~2021年2月28日我院骨科住院患者的病历及医嘱信息,基于PCNE分类系统对DRPs进行分析。结果共纳入209名骨科住院患者,识别出253个DRPs问题,平均DRPs发生率为1.21次/人。主要为治疗安全性问题,共192个(75.89%),其次为61个(24.11%)治疗有效性问题。DRPs原因分析中最主要的为剂型选择问题,共182例次(71.93),其次有36例次(14.23%)为药物选择问题。结论通过PCNE分类系统发现我院DRPs发生率较高,药师需针对剂型选择和药物选择等问题开展相应的药学服务,以促进骨科患者合理用药。
文摘目的:探讨iDose4迭代重建技术在学龄前儿童胸部CT低剂量平扫中的应用研究。方法:连续选取150例行胸部CT平扫2~6岁患儿,随机分为常规剂量组(50 mAs)、低剂量25 mAs组和低剂量10 mAs组。常规剂量组采用FBP重建作为常规对照组,低剂量扫描组分别采用FBP及iDose4-5对图像进行重建。2名放射科副主任医师对图像主观质量行盲法阅片,比较不同毫安秒和重建算法组图像主客观质量及辐射剂量并行统计学分析。结果:25 mAs iDose4组较25 mAs FBP组图像主客观质量增高,10 mAs iDose4组较10 mAs滤波反投影(FBP)组图像主客观质量增高。与常规剂量组比较,25 mAs FBP组图像主客观质量降低,25 mAs iDose4组图像主客观质量增高,10 mAs FBP组图像主客观质量降低,10 mAs iDose4组图像主客观质量差异无统计学意义。低剂量25 mAs、10 mAs组有效剂量较常规剂量组分别降低52.6%和78.2%。结论:行学龄前儿童胸部CT低剂量平扫时,采用25 mAs、10 mAs联合iDose4-5技术,能在降低辐射剂量的同时,获得与常规剂量更优或相当的图像质量。
基金funded by the Soybean Industrial Science and Technology System of China (Nycytx-004)
文摘The aim of the study was to establish a set of differential strains and to identify soybean resistant genes to Phytophthora root rot and then to apply those strains for analysis of the resistant genes Rps1a,Rps1c,and Rps1k that soybean cultivars or lines may carry.Virulence formula of 125 Phytophthora sojae isolates were determined using the hypocotyls inoculation technique,the strains,which includ 6 isolates with different virulence formulas,were applied to identify the resistance of 55 soybean cultivars or lines and resistant genes were analyzed using the gene postulating procedure.Eighteen reaction types occurred in 55 cultivars or lines and results of gene postulation indicated that 2 cultivars or lines probably carried gene Rps1c and no cultivar may carry genes Rps1a or Rps1k.A few of soybean cultivars or lines from Huanghuai Region carry Rps genes Rps1a,Rps1c and Rps1k and tend to infect by P.sojae,so resistant cultivars or lines need to be bred and popularized actively.
基金This work was funded by grants from the Fundamental Research Funds for the Central Universities,China(KYT202001 and JCQY201901)the Special Fund for Agro-scientific Research in the Public Interest,China(201503112).
文摘Foot and root rot caused by Phytophthora parasitica is a substantial threat to citrus cultivation,affecting both yield and quality.Thus,rapid and accurate detection of P.parasitica plays an important role in disease management.The aim of this study was to develop a simple diagnostic method to detect P.parasitica infection by combining recombinase polymerase amplification and lateral flow strips(LF-RPA).To establish the LF-RPA assay of P.parasitica,the primers and probe designed based on the Ypt1 gene were tested for specificity to P.parasitica,which showed no cross-reactivity with DNAs of other related oomycete species.The LF-RPA assay detected the amount of genomic DNA of P.parasitica which was as low as 1 pg.To make the LF-RPA assay useful in low-resource settings,four simplified DNA extraction methods were compared,after which the LF-RPA assay was applied,with no specialized equipment,to analyze a diverse range of citrus tissues by using a simplified PEG-NaOH method for DNA extraction.This method was successful in detecting P.parasitica in infected plant samples within 30 min.Combining the LF-RPA assay and a simplified DNA extraction method could be a potential detection test for P.parasitica,especially in areas with limited resources.
