Kunming mouse strain is widely used in China, and the superovulation was administrated with 10 IU PMSG combined with 10 IU hCG. In this study, the effects of the exogenous gonadotropins on superovulation of Kunming mi...Kunming mouse strain is widely used in China, and the superovulation was administrated with 10 IU PMSG combined with 10 IU hCG. In this study, the effects of the exogenous gonadotropins on superovulation of Kunming mice and embryo quality derived from the superovulated mice were assessed. Female mice at 6-8-wk old were superovulated with 0, 5, 7.5 and 10 IU PMSG/hCG and mated with male mice. The embryos were retrieved at 2.5 d post coitum. No statistic difference was observed for the number of 2-cell embryos collected per mouse between control and 5 IU PMSG/hCG treatment group, but the number significantly increased for 7.5 and 10 IU PMSG/hCG treatment group （P〈0.05）. The average number of 4- cell and 8-cell embryos collected from each mouse significantly differed between control and 5, 7.5, 10 IU PMSG/hCG treatment groups （P〈0.05）. When 8-cell embryos derived from mice administrated with 0, 5, 7.5 and 10 IU PMSG/hCG were cultured in KSOM, the blastocyst development rates were 88.1, 94.7, 96.1 and 94.3%, respectively, which were similar to control （P〉0.05）. This indicated that exogenous gonadotropins have no effects on development of Kunming mouse embryos. The quality of blastocyst was assessed by labelling with Hoechst and propidium iodide for inner cell mass and trophectoderm cells, the result showed that ICM/TE ratio significantly decreased for 10 IU PMSG/hCG treatment group compared with control, 5 and 7.5 IU PMSG/hCG treatment group （P〈0.05）. This suggested that the embryo quality of Kunming mouse has been affected by high dose of gonadotropins.展开更多
Highly efficient phosphorescent white organic light-emitting diodes （WOLEDs） with stable emission spectra are successfully fabricated by using an RGB three-color separately monochromatic emission layer （EML） struc...Highly efficient phosphorescent white organic light-emitting diodes （WOLEDs） with stable emission spectra are successfully fabricated by using an RGB three-color separately monochromatic emission layer （EML） struc- ture. The EML consists of a sequence of bis（2-methyldibenzo[f, h]quinoxaline） （acetylacetonate） iridium （Ⅲ） （Ir（MDQ）2（acac）） doped tris（4-carbazoyl-9-ylphenyl）amine （TCTA） as the red emission layer, iridium, tris（2- phenylpyidine）（Ir（ppy）3） doped TCTA as the green emission layer and iridium（Ill） Ibis（4, 6-difuorophenyl）- pyridinato-N, C2＇]picolinate （FIrpic） doped a mixed-host of TCTA and tris（4-carbazoyl-9-ylphenyl）amine （26DCz PPy） as the blue emission layer. Without using any out-coupling techniques, the resulting WOLEDs achieve a power efficiency of 421m/W at lOO cd/m2, and 34 lm/W at 1000 cd/m2. The WOLEDs also show excellent spec- trum stability with bias voltages, remaining the Commission Internationale de L＇Eclairage coordinates at （0.44, 0.43） from 1000 cd/m2 to 10000 cd/m2 and the color rendering index is as high as over 80. We contribute the sta- ble emission spectrum to the RGB separate EML structure that successfully suppresses the undesired competition between host-guest energy transfer and direct exciton formation on emissive dopants by effectively controlling the position of exciton recombination region.展开更多
基金supported by the Natural Science Foundation of Inner Mongolia for the Major Special Projects,China(2010ZD05)
文摘Kunming mouse strain is widely used in China, and the superovulation was administrated with 10 IU PMSG combined with 10 IU hCG. In this study, the effects of the exogenous gonadotropins on superovulation of Kunming mice and embryo quality derived from the superovulated mice were assessed. Female mice at 6-8-wk old were superovulated with 0, 5, 7.5 and 10 IU PMSG/hCG and mated with male mice. The embryos were retrieved at 2.5 d post coitum. No statistic difference was observed for the number of 2-cell embryos collected per mouse between control and 5 IU PMSG/hCG treatment group, but the number significantly increased for 7.5 and 10 IU PMSG/hCG treatment group （P〈0.05）. The average number of 4- cell and 8-cell embryos collected from each mouse significantly differed between control and 5, 7.5, 10 IU PMSG/hCG treatment groups （P〈0.05）. When 8-cell embryos derived from mice administrated with 0, 5, 7.5 and 10 IU PMSG/hCG were cultured in KSOM, the blastocyst development rates were 88.1, 94.7, 96.1 and 94.3%, respectively, which were similar to control （P〉0.05）. This indicated that exogenous gonadotropins have no effects on development of Kunming mouse embryos. The quality of blastocyst was assessed by labelling with Hoechst and propidium iodide for inner cell mass and trophectoderm cells, the result showed that ICM/TE ratio significantly decreased for 10 IU PMSG/hCG treatment group compared with control, 5 and 7.5 IU PMSG/hCG treatment group （P〈0.05）. This suggested that the embryo quality of Kunming mouse has been affected by high dose of gonadotropins.
基金Supported by the Science Fund for Creative Research Groups of the National Natural Science Foundation of China under Grant No 20921061, the National Natural Science Foundation of China under Grant Nos 50973104, 60906020, 21161160442, 61036007 and 60937001, the Ministry of Science and Technology of China under Grant Nos 2009CB623604 and 2009CB930603, and the Foundation of Jilin Research Council under Grant No 2012ZDGG001, 20090127 and 201105028.
文摘Highly efficient phosphorescent white organic light-emitting diodes （WOLEDs） with stable emission spectra are successfully fabricated by using an RGB three-color separately monochromatic emission layer （EML） struc- ture. The EML consists of a sequence of bis（2-methyldibenzo[f, h]quinoxaline） （acetylacetonate） iridium （Ⅲ） （Ir（MDQ）2（acac）） doped tris（4-carbazoyl-9-ylphenyl）amine （TCTA） as the red emission layer, iridium, tris（2- phenylpyidine）（Ir（ppy）3） doped TCTA as the green emission layer and iridium（Ill） Ibis（4, 6-difuorophenyl）- pyridinato-N, C2＇]picolinate （FIrpic） doped a mixed-host of TCTA and tris（4-carbazoyl-9-ylphenyl）amine （26DCz PPy） as the blue emission layer. Without using any out-coupling techniques, the resulting WOLEDs achieve a power efficiency of 421m/W at lOO cd/m2, and 34 lm/W at 1000 cd/m2. The WOLEDs also show excellent spec- trum stability with bias voltages, remaining the Commission Internationale de L＇Eclairage coordinates at （0.44, 0.43） from 1000 cd/m2 to 10000 cd/m2 and the color rendering index is as high as over 80. We contribute the sta- ble emission spectrum to the RGB separate EML structure that successfully suppresses the undesired competition between host-guest energy transfer and direct exciton formation on emissive dopants by effectively controlling the position of exciton recombination region.
