The color of watermelon flesh is an important trait determined by a series of carotenoids.Herein,we used Cream of Saskatchewan(pale yellow flesh)and PI 186490(white flesh)as parental materials for an F2 segregation an...The color of watermelon flesh is an important trait determined by a series of carotenoids.Herein,we used Cream of Saskatchewan(pale yellow flesh)and PI 186490(white flesh)as parental materials for an F2 segregation and initial mapping using the bulked segregant analysis sequencing(BSA-seq)strategy.The BSA results revealed a flesh colorrelated QTL that spans approximately 2.45 Mb on chromosome 6.This region was preliminarily positioned in a 382-kb segment,and then narrowed down into a 66.8-kb segment with 1260 F2 individuals.A total of nine candidate genes were in the fine mapping interval,but only Cla007528(encoding chlorophyllase)had non-synonymous mutations and was significantly expressed between the parental materials throughout flesh development.We also checked the expression patterns of the carotenoid metabolic pathway genes based on RNA-seq data and qRT-PCR validation.Three genes in the xanthophyll cycle(ClCHYB,ClNCED-1 and ClNCED-7)exhibited differential expression patterns between the two parental lines at different flesh color formation stages.ClPSY1,ClPDS,ClZDS,ClCHXE,ClCRTISO and ClLCYB also exhibited clearly different expression patterns accompanied by carotenoid accumulation.展开更多
基金This work was supported by the National Natural Science Foundation of China(31601775)the Project of China Postdoctoral Science Foundation(2017M611345)+1 种基金the earmarked fund for China Agriculture Research System of MOF and MARA(CARS-25)the Natural Science Foundation of Heilongjiang Province,China(C2017034).
文摘The color of watermelon flesh is an important trait determined by a series of carotenoids.Herein,we used Cream of Saskatchewan(pale yellow flesh)and PI 186490(white flesh)as parental materials for an F2 segregation and initial mapping using the bulked segregant analysis sequencing(BSA-seq)strategy.The BSA results revealed a flesh colorrelated QTL that spans approximately 2.45 Mb on chromosome 6.This region was preliminarily positioned in a 382-kb segment,and then narrowed down into a 66.8-kb segment with 1260 F2 individuals.A total of nine candidate genes were in the fine mapping interval,but only Cla007528(encoding chlorophyllase)had non-synonymous mutations and was significantly expressed between the parental materials throughout flesh development.We also checked the expression patterns of the carotenoid metabolic pathway genes based on RNA-seq data and qRT-PCR validation.Three genes in the xanthophyll cycle(ClCHYB,ClNCED-1 and ClNCED-7)exhibited differential expression patterns between the two parental lines at different flesh color formation stages.ClPSY1,ClPDS,ClZDS,ClCHXE,ClCRTISO and ClLCYB also exhibited clearly different expression patterns accompanied by carotenoid accumulation.
