异质性光照下克隆整合对白夹竹根际土壤细菌共现网络的影响

异质性光照下,克隆植物相连分株间光合产物的传输与共享可能影响分株根际土壤氮素转化过程,但是对调控该过程的细菌网络结构还认识不清.以根状茎克隆植物白夹竹为对象,研究克隆整合对遮荫分株的根际土壤细菌群落组成和共现网络结构特征.相比于根状茎切断处理,根状茎连接导致化能异养、需氧化能异养、硝化作用、好氧氨氧化、好氧亚硝酸盐氧化等与氮素转化过程相关细菌的丰度更高,功能共现网络的平均度和图密度更大,且边和节点数量更多.异质性光照下,克隆整合可能影响分株根际土壤微生物群落功能共现网络的复杂性,进而提升根际土壤氮素转化效率.

Clinical Efficacy of Ultrasonic Medicinal Penetration in the Removal of Blood Stasis and Alleviation of Zhuyu Juanbi Formula in the Treatment of Peripheral Neuropathy Induced by Paclitaxel

Objective: To observe the clinical efficacy and differences of the Zhuyu Juanbi formula delivered through ultrasound at Zusanli on patients with chemotherapy-induced peripheral neuropathy (CIPN) due to paclitaxel injection. Methods: A total of 72 breast cancer patients with CIPN were randomly divided into two groups. The treatment group (36 cases) was treated with oral methylcobalamin plus ultrasonic medicine permeating Zhuyu Juanbi formulae, while the control group (36 cases) was treated with oral methylcobalamin alone. Following two 2 cycles of continuous treatment, the efficacy of peripheral neurotoxicity, TCM syndrome score, FACT/GOG-Ntx score, total neuropathy score, and safety indicators of gynecological cancer patients were observed in the two groups. Result: In the treatment of CIPN, the addition of ultrasonic medicine permeating Zhuyu Juanbi formulae was more effective than oral methylcobalamin alone in reducing peripheral neurotoxicity and improving the quality of life of patients. The difference between the two groups was statistically significant (P < 0.05), and ultrasound drug penetration Zhuyu Juanbi formulae significantly reduced the FACT/ GOG-Ntx score and TNS score in the treatment group. In terms of drug safety, it rarely caused adverse reactions such as grade 3 and 4 leukopenia, and the safety profile was therefore good. Conclusion: The combination of ultrasonic medicine permeating Zhuyu Juanbi formulae and methylcobalamin has been demonstrated to be an effective treatment for peripheral neurotoxicity in patients with PIPN. It has been shown to significantly improve the clinical symptoms of PIPN patients, improve the quality of life of patients, and have a good safety profile.

Research Progress of Traditional Chinese Medicine External Treatment for Cancer Pain

Cancer pain,as a common complication in patients with malignant tumors,is regarded as the fifth vital sign.The involvement of traditional Chinese medicine(TCM)in the treatment of malignant tumors has become a distinctive feature of oncology treatment in China.It is also an important component of cancer pain management.TCM analgesic treatments include various methods,such as internal medicine and external therapies.External analgesic therapies,in particular,are significant methods in TCM pain management and offer both local treatment and systemic regulation.These methods are simple,easy to perform,and non-invasive.They can enhance pain relief effects while reducing the difficulty of oral medication intake and avoiding adverse gastrointestinal reactions,providing new perspectives and approaches for cancer pain treatment with broad development prospects.This article provides a review of the external TCM therapies for cancer pain to share with peers in the field.

基于体质健康测试的大学生身体素质影响因素研究

身体素质是人们体质强弱和健康状况的重要判断标志.精准把握学生体质与健康的影响因素以靶向施策.本研究探究了玉溪师范学院大学生身体素质的现状及其影响因素.基于2021年该校体质健康测试的数据运用描述性统计、差异性分析、相关性分析及回归分析等方法,分别以人口统计学因素和BMI为自变量,以身体素质(速度、下肢力量和柔韧性)为因变量,进行了综合分析.研究发现:超重和肥胖者比例较高且呈上升趋势,身体素质优秀者比例较低;性别、年级和学院与身体素质显著相关:BMI与立定跳远和坐位体前屈呈负相关;人口统计学因素和BMI能有效预测身体素质.研究建议高校从多角度采取预防性措施,提升学生的身体素质水平.

一种可预测TFT-LCD垂直串扰水平的定量分析方法

电容耦合和TFT漏电引起的垂直串扰问题在高分辨率液晶显示器产品上变得较为突出,大大影响了产品良率。业内通常采用VESA 2.0标准利用窗口画面测试串扰水平,但目前还没有一种预测串扰水平的定量分析方法。本文从垂直串扰形成机理出发,提出了一种可预测垂直串扰水平的定量分析方法,可预测出不同模式产品垂直串扰最严重的画面,有利于我们更好地研究和分析产品的品质。首先,通过分析垂直串扰机理,得到了垂直串扰现象与源电压差之间的定性对应关系。然后,通过分析V-T曲线,得到窗口画面下的亮度变化与源电压差之间的定量关系|ΔL|=kα|ΔV|。通过PCB板上的输出节点可以得到各灰阶对应的正负源电压,依据灰阶画面对应的源电压找到V-T曲线上对应的点可求出对应的斜率k值,依据漏电机理可求出对应的源电压差值|ΔV|,|ΔV|变化不大时可认为漏电电压降系数α为定值,故可计算出不同灰阶背景画面在窗口画面影响下的亮度变化值|ΔL|,将|ΔL|除以V-T曲线上对应灰阶的亮度值即可得到串扰值,通过比较不同窗口画面的串扰计算值即可得出串扰最严重的画面。最后,采用VESA 2.0标准方法测试不同窗口画面下的垂直串扰水平,与此方法的计算结果进行比较,串扰变化趋势吻合较好,TN和ADS模式下的线性相关系数分别达0.98和0.93以上。结果表明,此方法可以用来定量地研究产品垂直串扰的问题。

佛教“八正道”理念与运动训练原则根本价值同一性探析

本文通过对佛教"八正道"与运动训练原则阐释其哲学内涵的方式、方法进行比对研究,发现二者虽处于不同的学科领域,甚至存在宗教色彩,但对其概念的根本价值达成路径与模式看,二者是一致的。主要结论:佛教"八正道"与运动训练原则最终目标达成是以构建基于其理论背景的人的精神世界,是在客观基础上的主观认识,是客观实践的最终体现。

TFT-LCD画面闪烁影响因子及定量分析方法

画面闪烁(Flicker)是TFT-LCD画面品质评价的重要指标。文章基于ΔVp及闪烁发生机理,通过一系列实验,首先研究了Vcom对闪烁的影响,结果表明Vcom对闪烁具有显著影响;随着闪烁灰阶升高,最佳闪烁呈逐渐减小趋势,常白模式产品最佳闪烁对应Vcom逐渐减小,而常黑模式产品最佳闪烁对应Vcom逐渐增加。其次,研究了VGH、VGL对闪烁影响,结果表明VGH、VGL变化对闪烁均有显著影响;同一闪烁灰阶,VGH增加不会影响最佳闪烁,但对应Vcom逐渐减小,而VGL增加最佳闪烁逐渐减小,但对应Vcom逐渐增加。最后,提出一种可以预测不同灰阶闪烁水平的定量分析方法,通过该方法计算得到的闪烁水平与VESA标准下FMA测试法所得的闪烁值趋势一致,常白模式和常黑模式产品线性相关系数均达0.95以上,证明该方法可以用来预测不同灰阶闪烁水平。这些研究对改善TFT-LCD闪烁问题提供了分析方法和解决思路。

线粒体分裂抑制剂-1在香烟诱导小鼠气道炎症与氧化应激中的作用机制

目的 探索线粒体分裂抑制剂-1(Mdivi-1)在香烟诱导小鼠气道炎症与氧化应激中的作用及相关的作用机制。方法 香烟暴露构建小鼠气道炎症与氧化应激动物模型,腹腔注射Mdivi-1进行干预,收集肺泡灌洗液进行细胞分类计数,ELISA检测肺泡灌洗液炎症因子TNF-α、IL-6及MMP-9水平,生化试剂盒测定肺组织匀浆氧化应激指标MDA含量及SOD的活性,HE染色评估肺组织病理改变。在PubChem数据库中检索Mdivi-1作用靶点基因,对基因进行GO和KEGG分析,Western blot检测小鼠肺组织Drp1和Mfn2的表达变化及相关富集的通路。结果 熏烟诱导小鼠气道中的炎症细胞浸润增加、气道上皮细胞过度增生、气道上皮增厚,增加肺泡灌洗液中总细胞、中性粒细胞、巨噬细胞数量及炎症因子TNF-α、IL-6及MMP-9水平,增强氧化应激反应,而Mdivi-1预处理可以减轻上述改变。生物信息分析显示Mdivi-1潜在作用基因100个,GO分析显示富集到线粒体结构调节、凋亡过程、线粒体自噬等生物过程,KEGG分析显示富集到的主要通路为Tnf、Mtor、Ampk、Adipocytokine及Nod-Like受体信号通路。Western blot显示Mdivi-1干预抑制香烟诱导小鼠肺组织NF-κB信号通路激活,下调香烟诱导的Drp1表达并上调Mfn2表达。结论 Mdivi-1缓解香烟诱导的小鼠气道炎症和氧化应激,其机制可能通过改善线粒体功能和抑制NF-κB信号通路活化实现,Mdivi-1有可能是治疗香烟诱导的气道炎症性疾病的新药物。

CHIP regulates bone mass by targeting multiple TRAF family members in bone marrow stromal cells

Carboxyl terminus of Hsp70-interacting protein（CHIP or STUB1） is an E3 ligase and regulates the stability of several proteins which are involved in different cellular functions. Our previous studies demonstrated that Chip deficient mice display bone loss phenotype due to increased osteoclast formation through enhancing TRAF6 activity in osteoclasts. In this study we provide novel evidence about the function of CHIP. We found that osteoblast differentiation and bone formation were also decreased in Chip KO mice. In bone marrow stromal（BMS） cells derived from Chip^-/- mice, expression of a panel of osteoblast marker genes was significantly decreased. ALP activity and mineralized bone matrix formation were also reduced in Chip-deficient BMS cells. We also found that in addition to the regulation of TRAF6, CHIP also inhibits TNFα-induced NF-κB signaling through promoting TRAF2 and TRAF5 degradation. Specific deletion of Chip in BMS cells downregulated expression of osteoblast marker genes which could be reversed by the addition of NF-κB inhibitor. These results demonstrate that the osteopenic phenotype observed in Chip^-/- mice was due to the combination of increased osteoclast formation and decreased osteoblast differentiation. Taken together, our findings indicate a significant role of CHIP in bone remodeling.

Inhibition of Axinl in osteoblast precursor cells leads to defects in postnatal bone growth through suppressing osteoclast formation

Axinl is a negative regulator of β-catenin signaling and its role in osteoblast precursor cells remains undefined.In the present studies,we determined changes in postnatal bone growth by deletion of Axinl in osteoblast precursor cells and analyzed bone growth in newborn and postnatal Axin1 O5X mice and found that hypertrophic cartilage area was largely expanded in AxinlOSX KO mice.A larger number of chondrocytes and unabsorbed cartilage matrix were found in the bone marrow cavity of Axin1OSX KO mice.Osteoclast formation in metaphyseal and subchondral bone areas was significantly decreased,demonstrated by decreased TRAPpositive cell numbers,associated with reduction of MMP9-and cathepsin K-positive cell numbers in Axin1 O5X KO mice.OPG expression and the ratio of O p g to Rankl were significantly increased in osteoblasts of Axinl O5X KO mice.Osteoclast formation in primary bone marrow derived microphage(BMM)cells was significantly decreased when BMM cells were cultured with conditioned media(CM)collected from osteoblasts derived from Axin1OSX mice compared with BMM cells cultured with CM derived from WT mice.Thus,the loss of Axinl in osteoblast precursor cells caused increased OPG and the decrease in osteoclast formation,leading to delayed bone growth in postnatal Axin1°sx KO mice.

Nociceptive behavioural assessments in mouse models of temporomandibular joint disorders

Orofacial pain or tenderness is a primary symptom associated with temporomandibular joint(TMJ)disorders(TMDs).To understand the pathological mechanisms underlying TMDs,several mouse models have been developed,including mechanical stimulus-induced TMD and genetic mouse models.However,a lack of feasible approaches for assessing TMD-related nociceptive behaviours in the orofacial region of mice has hindered the in-depth study of TMD-associated mechanisms.This study aimed to explore modifications of three existing methods to analyse nociceptive behaviours using two TMD mouse models:(1)mechanical allodynia was tested using von Frey filaments in the mouse TMJ region by placing mice in specially designed chambers;(2)bite force was measured using the Economical Load and Force(ELF)system;and(3)spontaneous feeding behaviour tests,including eating duration and frequency,were analysed using the Laboratory Animal Behaviour Observation Registration and Analysis System(LABORAS).We successfully assessed changes in nociceptive behaviours in two TMD mouse models,a unilateral anterior crossbite(UAC)-induced TMD mouse model and aβ-catenin conditional activation mouse model.We found that the UAC model andβ-catenin conditional activation mouse model were significantly associated with signs of increased mechanical allodynia,lower bite force,and decreased spontaneous feeding behaviour,indicating manifestations of TMD.These behavioural changes were consistent with the cartilage degradation phenotype observed in these mouse models.Our studies have shown reliable methods to analyse nociceptive behaviours in mice and may indicate that these methods are valid to assess signs of TMD in mice.

Clinical characteristics of intrahepatic biliary papilloma:A case report

BACKGROUND Intrahepatic bile duct papilloma(IPNB)is a rare benign tumour from the bile duct epithelium and has a high malignant transformation rate.Early radical resection can obviously improve the prognosis of patients,but it is difficult to be sure of the diagnosis of IPNB before operating.CASE SUMMARY This study included 28 patients with intraductal papilloma admitted to the First Hospital of Jilin University from January 2010 to November 2020 and recorded their clinical manifestations,imaging features,complications and prognosis.There were 12 males and 16 females with an average age of 61.36±8.03 years.Most patients had symptoms of biliary obstruction.Biliary dilatation and cystic mass could be seen on imaging.After surgery,IPNB was diagnosed by pathology.CONCLUSION IPNB is a rare benign tumour in the bile duct.Early diagnosis and timely R0 resection can improve the prognosis of IPNB.

《云南高校户外运动课程风险评估量表》的创建和信效度检验

我国户外运动市场正迎来高速发展阶段,越来越多高校已将户外运动纳入课程体系。高风险、难以预料的特征使得此类课程的安全性成为焦点,必须采取措施进行预防,以此促进高校户外运动课程实现良性发展。本文采用文献法构建六维度《云南高校户外运动课程风险评估量表》,使用问卷调查法和数理统计法检测量表的信度和结构效度,结果表明:(1)量表具有良好的信度;(2)探索性因子分析聚合出的6个公因子数与初始设计一致;(3)验证性因子分析构建的结构方程模型的适配度指标符合标准值;(4)量表的聚合效度和区分效度良好。研究证明:该量表具有良好的信度和效度,可以作为云南省户外运动课程开展风险的有效预测工具。

Decreased Sperm Quality in Mice Fed a Diet with Fish Oil

Polyunsaturated fatty acids （PUFAs） play an important role in sperm quality and fertility. This study was conducted to investigate the effect of dietary supplementation with PUFAs on male mice reproductive capacity. Mice were fed a diet of 4% soybean oil （SO） ,4% fish oil （FO） ,or 4% conjugated linoleic acid （CLA） for 30 days. Litter sizes and sperm quality were measured during the study. Litter size decreased in females mated with FO group males. Although sperm total number,motility,and in virto fertilization did not differ among treatments,the proportion of intact sperm membrane decreased in the FO group compared to other groups. This was supported by the increased proportion of damaged sperm membrane inthe FO group. Furthermore,the percentage of high mitochondrial membrane potential （MMP） declined,but the percentage of low MMP was increased by dietary FO and CLA supplementation compared to the SO group. Sperm membrane phospholipid in mice receiving the FO diet had a higher concentration of docosapentenoic acid （C22 ∶ 5n-3,DPA） ,docosahexaneoic acid （C22∶ 6n-3,DHA） ,and n-3 PUFAs,but lower levels of arachidonic acid （C20 ∶ 4n-6,AA） and n-6 PUFAs compared to those receiving the SO diet. These data suggest that decreased sperm quality in mice fed a FO diet may be due to excessive DPA and DHA in the membrane.

Microemulsion Polymerization of Methyl Methacrylate

The microemulsion polymerization of methyl methacrylate was studied. The effects of feeding modes on the structure and the properties of the obtained polymer microlatex were investigated by measuring the conversion, the transmittance and the refractive index of the latex, and by measuring the particle size, the molecular weight and the glass transition temperature (T_g) of the polymers. The results show that compared to the batch feeding mode, the semi-continuous feeding mode is more favorable to form a PMMA microlatex with a higher transmittance, a smaller particle size, a higher molecular weight and a higher T_g. And the obtained PMMA microlatex has a 30%—40% (mass fraction) polymer content, a 0. 03 emulsifier/water weight ratio, a 0. 05 emulsifier/monomer weight ratio and a 17 nm average particle diameter, which is very important for the industrialization of the microemulsion polymerization technique.

Clinical characteristics of adult-type annular pancreas:A case report

BACKGROUND Annular pancreas(AP)is a rare congenital abnormal rotation of the pancreas.AP rarely occurs in adults.Pancreatic tumors and ampullary tumors are related to AP,so the discovery and treatment of AP are essential.CASE SUMMARY This study investigated the clinical manifestations,imaging features,complications,and treatment of six patients diagnosed with AP at the Department of Hepatobiliary and Pancreatic Surgery,First Hospital of Jilin University from January 2010 to June 2020.There were four males and two females,with an average age of 56.00±9.86 years old.In this study,abdominal pain and jaundice were the main clinical manifestations.Imaging can show the“crocodile jaw sign”or“double bubble sign”.CONCLUSION For patients with duodenal or biliary obstruction,physicians should give priority to AP when imaging examinations suggest that the duodenum is wrapped with tissue similar to the density of the pancreas.Symptomatic patients should actively undergo surgical treatment.

Deletion of Axin1 in aggrecan-expressing cells leads to growth plate cartilage defects in adult mice

In mammals,skeletal bone development begins at hyaline cartilage formation at the early embryonic stage.1Then osteoblasts from the periosteum,which are distributed surrounding the hyaline cartilage,build up compact bone to formdiaphysis and spongy bone known as primary ossification center.1 Next,osteoclasts from the hematopoietic system destroy spongy bone to generate a medullary cavity.

Autophagy and tight junction proteins in the intestine and intestinal diseases

The intestinal epithelium(IE) forms an indispensible barrier and interface between the intestinal interstitium and the luminal environment. The IE regulates water, ion and nutrient transport while providing a barrier against toxins, pathogens(bacteria, fungi and virus) and antigens. The apical intercellular tight junctions(TJ) are responsible for the paracellular barrier function and regulate transepithelial flux of ions and solutes between adjacent cells. Increased intestinal permeability caused by defects in the IE TJ barrier is considered an important pathogenic factor for the development of intestinal inflammation, diarrhea and malnutrition in humans and animals. In fact, defects in the IE TJ barrier allow increased antigenic penetration, resulting in an amplified inflammatory response in inflammatory bowel disease(IBD), necrotizing enterocolitis and ischemia-reperfusion injury. Conversely, the beneficial enhancement of the intestinal TJ barrier has been shown to resolve intestinal inflammation and apoptosis in both animal models of IBD and human IBD. Autophagy(self-eating mechanism) is an intracellular lysosome-dependent degradation and recycling pathway essential for cell survival and homeostasis.Dysregulated autophagy has been shown to be directly associated with many pathological processes,including IBD. Importantly, the crosstalk between IE TJ and autophagy has been revealed recently. We showed that autophagy enhanced IE TJ barrier function by increasing transepithelial resistance and reducing the paracellular permeability of small solutes and ions, which is, in part, by targeting claudin-2,a cation-selective, pore-forming, transmembrane TJ protein, for lysosome(autophagy)-mediated degradation. Interestingly, previous studies have shown that the inflamed intestinal mucosa in patients with active IBD has increased claudin-2 expression. In addition, inflammatory cytokines(for example,tumor necrosis factor-α, interleukin-6, interleukin-13, and interleukin-17) whose levels are increased in IBD patients cause an increase in claudin-2 expression and a claudin-2-dependent increase in TJ permeability. Thus, the role of claudin-2 in intestinal pathological processes has been attributed, in part, to the increase of intestinal TJ permeability. Claudin-2 represents a new therapeutic target in treating IBD,diarrhea and malnutrition in animals and humans.

Integrated scanning spectrometer with a tunable micro-ring resonator and an arrayed waveguide grating

Integrated spectrometers with both wide optical bandwidths and high spectral resolutions are required in applications such as spectral domain optical coherence tomography(SD-OCT).Here we propose a compact integrated scanning spectrometer by using a tunable micro-ring resonator(MRR)integrated with a single arrayed waveguide grating for operation in the 1265-1335-nm range.The spectral resolution of the spectrometer is determined by the quality factor of the MRR,and the optical bandwidth is defined by the free spectral range of the arrayed waveguide grating.The spectrometer is integrated with on-chip germanium photodetectors,which enable direct electrical readout.A 70-nm optical bandwidth and a 0.2-nm channel spacing enabled by scanning the MRR across one free spectral range are demonstrated,which offer a total of350 wavelength channels with 31-kHz wavelength scanning speed.The integrated spectrometer is applied to measure different spectra and the interference signals from an SD-OCT system,which shows its great potential for future applications in sensing and imaging systems.

Optimization of Hybrid Crystal with SAPO-5/34 on Hydrothermal Stability for deNOx Reaction by NH3

In order to deal with the challenge of the hydrothermal deactivation of selective catalytic reduction of NO by NH3(NH3-SCR)catalyst and extend its lifetime,a novel Cu/SAPO-5/34 catalyst was prepared,and it almostmaintains its deNOx performance with a high conversion rate of 90%NO,between 175℃and 400℃after under-going the rigorous treatment at 800℃for 12 h.Thus,Cu/SAPO-5/34 is more recalcitrant to the high-temperature hydrothermal deactivation than Cu/SAPO-34.Besides,the formation of N20 is always below 3×10^-6(3 ppm)during the whole reaction temperature.performing an advanced catalytic selectivity.The effect of high-temperature hvdro-thermal treatment on the morphology,structure and texture property,the acid sites,as well as the active copper spe cies were investigated.These characterizations manifest that the optimized high-temperature hydrothermal stability is associated closely with the good structural stability over Cu/SAPO-5/34-HT.which facilitates to preserve reaction sites,and then showing the better hydrothermal stability than Cu/SAPO-34.