Differential mRNA expression in peripheral blood is associated with oral squamous cell carcinoma:Recent advances and future challenges

Oral squamous cell carcinoma(OSCC)is a malignant tumor triggered by the accumulation of multiple gene mutations in oral epithelial cells.Different OSCC-related biomarkers have been reported in circulation in the peripheral blood that support the occurrence and development of OSCC.Recent advances in high-throughput and highly sensitive detection methods have overcome the limitation of the low concentration of most peripheral blood biomarkers.Hence,blood biomarker detection has become an efficient screening tool for the early diagnosis of OSCC.The growing data available in public cancer and gene databases have provided new foundations for OSCC research.In particular,the identification of OSCC biomarkers using bioinformatic tools has shed new light on the underlying mechanisms as well as on the genetic landscape of OSCC.More recently,mRNA targeting therapies have emerged as valuable anticancer treatment strategies,as they allow for the regulation of the expression of certain functional proteins to reverse genetic abnormalities or induce tissue repair.Thus,mRNA-targeting therapies can be used to regulate the expression of antigens,antibodies,or cellular receptors by immune cells.Particularly,anti-cancer cellular immunotherapy carrying specific mRNAs has attracted significant attention in OSCC treatment.Here,we review the present knowledge on the role of peripheral blood mRNAs in the diagnosis,treatment,development,and prognosis of OSCC.Moreover,we address future research prospects of mRNAs in the peripheral blood in OSCC and the opportunities and challenges that may arise in future clinical therapeutic applications.

植物B染色体的分子结构组成及遗传机制研究进展

B染色体(B chromosomes, Bs)是独立于常规染色体即A染色体(A chromosomes, As)的一类特殊染色体。长期以来,由于实验方法的限制和B染色体可有可无的性质,B染色体一直被认为是完全由异染色质组成且不携带任何功能基因。随着高通量测序技术的发展,很多物种的B染色体被分离,通过对其DNA组成进行全面分析,发现B染色体也携带蛋白质编码基因且对宿主的转录和蛋白表达有影响。本文以黑麦(Secale cereale L.)、玉米(Zea mays L.)和拟斯卑尔脱山羊草(Aegilops speltoides Tausch.)为例,主要介绍了B染色体对植物表型的影响、B染色体的分子结构及DNA重复序列组成、B染色体的功能基因研究,并讨论了植物B染色体领域的研究现状及潜在的应用前景,旨在为染色体工程和育种提供新思路。

一例ZMPSTE24基因复合杂合突变导致颅骨下颌骨皮肤发育不全B型的诊断和基因检测分析

颅骨下颌骨皮肤发育不全(mandibuloacral dysplasia,MAD)是一种罕见的常染色体隐性遗传疾病,主要与LMNA及ZMPSTE24基因发生变异有关。本文报道了国内首例颅骨下颌骨皮肤发育不全B型患者,主要表现为特殊面容、喂养困难、体格生长明显落后、全面的发育迟缓伴牙齿和骨骼发育异常。全外显子组家系测序结果显示患者携带ZMPSTE24基因c.743C>T(p.Pro248Leu)(dbSNP:rs121908095)与1~10号外显子缺失的复合杂合突变,经Sanger测序与定量聚合酶链反应(real-time quantitative PCR,RT-qPCR)实验检测显示,两个突变分别遗传自其表型正常的母亲与父亲。通过总结分析该例病例特点及其家系遗传规律,对于临床上难以进行明确诊断的疾病,建议进行全外显子组家系测序,辅助疾病的诊断。该病例的发现提高了临床医师对MAD疾病的认识,也为该疾病后续的遗传学研究提供新的临床资料。

大学生创新创业模式下陶瓷艺术设计基础课程教学的探讨与实践--以景德镇陶瓷大学为例

随着“大众创新、万众创业”战略的推出,各相关扶持政策相继出台,大学生创新创业热情高涨,大学生创新创业模式的引入作为课堂教学的补充,对我校教育事业的发展起到了积极的作用。本文将大学生创新创业模式融入到我校陶艺基础课程中,并对这种模式的应用进行分析探讨,旨在促进大学生创新创业模式教学的完善,提高我校陶艺基础课程教学水平及人才培养水平,实现学生的全面发展。

Identification and validation of a major gene for kernel length at the P1 locus in Triticum polonicum

Kernel size, one of the traits that determine wheat yield, is controlled by multiple quantitative trait loci.Polish wheat(Triticum polonicum) has elongated and plump kernel and is a valuable material for breeding high-yielding wheat cultivars. However, genes or loci determining kernel length(KL) in Polish wheat are unknown. We identified and validated a major KL gene, KL-PW, at the P1 locus in Polish wheat. KL-PW is VRT-A2, which encodes a MIKC-type MADS-box protein(MADS55). An insertion/deletion mutation in intron 1 of VRT-A2;led to an alternatively spliced transcript, VRT-A2;. Quantitative PCR analysis showed that VRT-A2;was more highly expressed in developing seeds than was VRT-A2 Ailanmai.Brassinosteroid(BR) sensitivity experiment and the expression of BR-related genes indicated that VRTA2;functions as a positive regulator of BR responses. VRT-A2;significantly increased KL of wheat.These findings not only reveal the molecular basis of KL-PW in controlling KL, but also provide a valuable genetic resource for increasing kernel size in wheat.

Effects of Paeonol on Expression of Type Ⅰ and Type Ⅲ Collagen of High Glucose Induced Proliferation of Cardiac Fibroblasts

[Objectives]To explore the effects of paeonol on the inhibition of myocardial fibrosis in high glucose induced cardiac fibroblasts(CFs).[Methods]Differential adherence method was used to culture the primary CFs of neonatal rats(passage culture);CCK-8 method was used to detect the cell proliferation;MTT assay was used to screen the safe concentration of paeonol;the 2 nd to 3 rd generation CFs were randomly divided into normal group(5.5 mmol/L,expressed in C),high glucose group(30 mmol/L,expressed in HG),paeonol low dose group(Pae-L,17.5 mg/L),and medium dose paeonol group(Pae-M,35 mg/L),paeonol high dose group(Pae-H,70 mg/L);Western Blot method was used to detect the expression of Col-I and Col-III protein.[Results]The extraction of CFs from primary neonatal rats was successful;high glucose(30 mmol/L)induction had a significant proliferation effect on CFs;compared with the normal group,the expressions of COI-I and Col-III protein were increased in the high glucose group(P<0.05);compared with the high glucose group,the expression of COI-I protein was decreased in each treatment group(P<0.05,P<0.01),and the decrease was most significant in the high dose group(P<0.01);the expression of COI-III in the high dose group was decreased and it was statistically significant(P<0.05).[Conclusions]Paeonol significantly inhibited the proliferation of high glucose induced CFs in neonatal rats.This experiment is intended to provide a new experimental basis for the prevention and treatment of diabetic cardiomyopathy(DCM).

Protective Effect of Paeonol on Diabetic Cardiomyopathy in Rats

[Objectives] The protective effect of paeonol on DCM was further confirmed by observing the changes of myocardial structure and function in rats. [Methods] Rats were fed high-sugar and high-fat diet and injected intraperitoneally with streptozotocin(STZ) for two consecutive days to prepare animal model of type II diabetes. The model rats were randomly divided into model group, low-dose paeonol group(30 mg/kg), middle-dose paeonol group(60 mg/kg), high-dose paeonol group(120 mg/kg) and metformin group(157 mg/kg). The detected indicators included heart weight index(HWI), blood glucose(FBG), body mass, triglyceride(TG), and total cholesterol(TC). Myocardial pathological changes were observed by HE staining. The extent of myocardial fibrosis was observed by Masson staining. [Results] In the model group, the body weight of the rats declined significantly, and the phenomena of overdrinking, overeating and polyuria occurred. Compared with the model group, the body weight of rats in the paeonol treatment groups increased to different extents. Compared with the normal group, the HWI, FBG, LDH, AST, and TG increased in the model group, and decreased in different degrees in the paeonol treatment groups(P<0.05), suggesting that paeonol has certain protective effect on diabetic cardiomyopathy in rats. After HE staining and fixation, the myocardial tissue of rats was observed by optical microscope. The morphology of myocardial cells and myocardial fibers in the normal group were normal;Compared with the normal group, the model group has hypertrophy of myocardial cells, disordered arrangement of myocardial fibers, fiber breakage and dissolution, uneven staining between myocardial cells, and nucleus breakage or even disappearance;Compared with the model group, the pathological changes of myocardium in the paeonol treatment groups and DMBG group have been improved to different degrees. The arrangement of myocardial fibers is regular and the coloration is uniform. The results of Masson staining showed that in the normal group, and there was no significant increase of myocardial collagen fibers in the myocardial interstitium. Compared with the normal group, myocardial cells and interstitial collagen fibers(blue) in the model group were significantly increased. Compared with model group, myocardial collagen fiber hyperplasia in the paeonol treatment groups and DMBG group showed different degrees of improvement, among which the Pae-H group had the least blue bands and the most obvious improvement of collagen fiber. [Conclusions] Paeonol has different degrees of improvement effects on myocardial fibrosis in rats with diabetic cardiomyopathy, suggesting that paeonol has a protective effect on diabetic cardiomyopathy in rats.

党的民族工作与中华民族大团结:历史及经验

中国共产党和党的历代领导人都非常重视中华民族大团结,历来将其作为党的统一战线工作尤其是民族工作的重要内容。新中国成立前,中国共产党增强中华民族大团结的主要举措是开展民族制度建设,以构筑制度保障实现中华民族大团结。新中国成立后,围绕国家建设和社会主义制度建设的各项任务,党把民族工作摆在突出重要位置,将民族平等作为立国的基本原则之一,将维护国家统一、民族团结、社会稳定作为各族人民的最高利益,采取了一系列重要措施,进一步促进了各民族共同团结奋斗、共同繁荣发展局面的形成。党的十八大以来,以习近平同志为核心的党中央统筹“两个大局”,立足实现中华民族伟大复兴的历史方位,从实现中华民族根本利益和最高利益的角度,赋予了中华民族大团结新的时代内涵和目标要求。纵观党的百年历程,始终强调国家统一和民族团结这条主线是党推进中华民族大团结的主要经验,这为新时代实现中华民族伟大复兴提供了重要启示。

Onto CSD: an ontology-based security model for an integrated solution of cyberspace defense

The construction of an integrated solution for cyberspace defense with dynamic, flexible, and intelligent features is a new idea. To solve the problem whereby traditional static protection methods cannot respond to various network attacks or security demands in an adversarial network environment in time, and to form a complete integrated solution from “threat discovery” to “decision-making generation,” we propose an ontology-based security model, Onto CSD, for an integrated solution of cyberspace defense that uses Web ontology language(OWL) to represent the ontology classes and relationships of threat monitoring, decision-making, response, and defense in cyberspace, and uses semantic Web rule language(SWRL) to design the defensive reasoning rules. Onto CSD can discover potential relationships among network attacks, vulnerabilities, the security state, and defense strategies. Further, an artificial intelligence(AI) expert system based on case-based reasoning(CBR) is used to quickly generate a detailed and comprehensive decision-making scheme. Finally, through Kendall ' s coefficient of concordance(W) and four experimental cases in a typical computer network defense(CND) system, which reasons on represented facts and the ontology, Onto CSD ' s consistency and its feasibility to solve the issues in the field of cyberspace defense are validated. Onto CSD supports automatic association and reasoning, and provides an integrated solution framework of cyberspace defense.

A human monoclonal antibody neutralizes SARS-CoV-2 Omicron variants bytargeting the upstream region of spike protein HR2 motif

The continuous emergence of new severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)variants meansthere is a need to explore additional strategies to develop broad-spectrum vaccines or therapeutics for individuals remaining at risk of coronavirus disease 2019(COVID-19).Neutralizing monoclonal antibody(mAb)that binds to theconserved S2 subunit of the SARS-CoV-2 spike(S)protein alone,or in combination with mAb that binds to the receptor-binding domain(RBD)of S protein,might be effective in eliciting protection from infection by a variety of SARS-CoV2 variants.Using high-throughput single-cell immunoglobulin sequencing of B cells from COVID-19-convalescent donors,we identified a high-affinity S2-specific mAb-39,that could inhibit original SARS-CoV-2 strain,Omicron BA.1,BA.2.86,BA.4,BA.5,and EG.5.1 S protein-mediated membrane fusion,leading to the neutralization of these pseudoviralinfections.Moreover,mAb-39 could also improve the neutralizing activity of anti-RBD antibody against the highlyneutralization-resistant Omicron variants.Molecular docking and point mutation analyses revealed that mAb-39 recognized epitopes within the conserved upstream region of the heptad repeat 2(HR2)motif of the S2 subunit.Collectively,these findings demonstrate that targeting the conserved upstream region of the HR2 motif(e.g.,using mAbs)provides anovel strategy for preventing the infection of SARS-CoV-2 and its variants.

Identification of two migratory colon ILC2 populations differentially expressing IL-17A and IL-5/IL-13

Group 2 innate lymphoid cells(ILC2s)play important tissue resident roles in anti-parasite immunity,allergic immune response,tissue homeostasis,and tumor immunity.ILC2s are considered tissue resident cells with little proliferation at steady state.Recent studies have shown that a subset of small intestinal ILC2s could leave their residing tissues,circulate and migrate to different organs,including lung,liver,mesenteric LN and spleen,upon activation.However,it remains unknown whether other ILC populations with migratory behavior exist.In this study,we find two major colon ILC2 populations with potential to migrate to the lung in response to IL-25 stimulation.One subset expresses IL-17A and resembles inflammatory ILC2s(iILC2s)but lacks CD27 expression,whereas the other expresses CD27 but not IL-17A.In addition,the IL-17A^(+)ILC2s express lower levels of CD127,CD25,and ST2 than CD27^(+)ILC2s,which express higher levels of IL-5 and IL-13.Surprisingly,we found that both colon ILC2 populations still maintained their colonic features of preferential expression of IL-17A and CD27,IL-5/IL-13,respectively.Together,our study identifies two migratory colon ILC2 subsets with unique surface markers and cytokine profiles which are critical in regulating lung and colon immunity and homeostasis.

Artificial intelligence algorithms for cyberspace security applications:a technological and status review

Three technical problems should be solved urgently in cyberspace security:the timeliness and accuracy of network attack detection,the credibility assessment and prediction of the security situation,and the effectiveness of security defense strategy optimization.Artificial intelligence(AI)algorithms have become the core means to increase the chance of security and improve the network attack and defense ability in the application of cyberspace security.Recently,the breakthrough and application of AI technology have provided a series of advanced approaches for further enhancing network defense ability.This work presents a comprehensive review of AI technology articles for cyberspace security applications,mainly from 2017 to 2022.The papers are selected from a variety of journals and conferences:52.68%are from Elsevier,Springer,and IEEE journals and 25%are from international conferences.With a specific focus on the latest approaches in machine learning(ML),deep learning(DL),and some popular optimization algorithms,the characteristics of the algorithmic models,performance results,datasets,potential benefits,and limitations are analyzed,and some of the existing challenges are highlighted.This work is intended to provide technical guidance for researchers who would like to obtain the potential of AI technical methods for cyberspace security and to provide tips for the later resolution of specific cyberspace security issues,and a mastery of the current development trends of technology and application and hot issues in the field of network security.It also indicates certain existing challenges and gives directions for addressing them effectively.

浙江美丽乡村空间格局及可持续发展模式研究

浙江美丽乡村建设系全国稳步推进乡村振兴战略的地方经典模式。基于浙江2016年以来3个批次美丽乡村“示范村”数据,运用洛伦兹曲线、平均最近邻指数、核密度分析和地理探测器等方法定量识别美丽乡村空间特征及影响因素,并构建乡村振兴可持续发展模式,揭示具有强烈资源禀赋约束的各类乡村如何在政策与资本双重驱动下从示范走向全域。结果表明:(1)美丽乡村示范村空间上整体呈凝聚型分布,已形成“杭嘉湖”和“金衢”两大集聚区,且其时空演化特征稳中有变,后两批较第一批分布范围更广;(2)美丽乡村具有农业服务型、工业发展型、休闲旅游型、文化传承型和综合治理型等五大类型,不同类型的空间分布特征差异显著,且与已有要素禀赋和产业基础关联密切;(3)区位条件、文化旅游资源、经济发展水平和地形是影响示范村区位的重要因素;(4)美丽乡村建设从局部到全域推进过程遵循优先发展资源禀赋示范村、辐射引领周边村落的路径,并倡导政府引导、资本驱动、“农户+公司”和村民创业并存的多元主体发展模式。

小桐子半胱氨酸蛋白酶家族和相应miRNAs的鉴定及其对低温锻炼的响应

小桐子(Jatropha curcas)是一种极具潜力的能源植物及冷敏植物, 12°C低温锻炼可显著提高其耐冷性。在全基因组水平上对小桐子半胱氨酸蛋白酶家族及靶向其基因的miRNAs进行鉴定、生物信息学分析和表达特性分析,并对该基因家族成员与miRNAs互作参与调控小桐子对低温锻炼的响应进行解析。结果表明,在小桐子基因组中共鉴定到39个半胱氨酸蛋白酶基因,定位于11条染色体上,可分为6个亚家族(C1A、C2、C12、C13、C14和C15),编码181–2 158个氨基酸残基的多肽,均具有Cys和His活性位点。基于miRNA组和降解组测序结果,发现有283个miRNAs靶向调控小桐子半胱氨酸蛋白酶基因家族的14个成员。对靶向JcDEK1、JcRD21B和JcXBCP3L的miRNAs在12°C低温锻炼过程中的共表达分析表明,这些miRNAs参与半胱氨酸蛋白酶基因表达的调控,且这种调控可能与低温锻炼诱导的小桐子耐冷性增强有关。研究结果有助于深入理解小桐子半胱氨酸蛋白酶基因家族功能及其与相应miRNAs的互作,以及通过互作调控小桐子对低温的响应。

An aqueous zinc-ion hybrid super-capacitor for achieving ultrahigh-volumetric energy density

Zinc-ion hybrid super-capacitors are regarded as promising safe energy storage systems,However,the relatively low volumetric energy density has become the main bottlenecks in practical applications of portable electronic devices,In this work,the zinc-ion hybrid super-capacitor with high volumetric energy density and superb cycle stability had been constructed which employing the high-density threedimensional graphene hydrogel as cathode and Zn foil used as anode in 1 mol/L ZnSO4 electrolyte.Benefiting from the abundant ion transport paths and the abundant active sites for graphene hydrogel with high density and porous structure,the zinc-ion hybrid super-capacitor exhibited an extremely high volumetric energy density of 118.42 Wh/L and a superb power density of 24.00 kW/L,as well as an excellent long cycle life(80% retention after 30,000 cycles at 10 A/g),which was superior to the volumetric energy density of the reported zinc-ion hybrid super-capacitors.This device,based on the fast ion adsorption/deso rption on the capacitor-type graphene cathode and reversible Zn^(2+) plating/stripping on the battery-type Zn anode,which will inspire the development of zinc-ion hybrid super-capacitor in miniaturized devices.

Microarray Analysis of Gene Expression Changes in Neuroplastin 65-Knockout Mice: Implications for Abnormal Cognition and Emotional Disorders

Neuroplastin 65 （Np65） is an immunoglobulin superfamily cell adhesion molecule involved in synaptic formation and plasticity. Our recent study showed that Np65-knockout （KO） mice exhibit abnormal cognition and emotional disorders. However, the underlying mechanisms remain unclear. In this study, we found 588 differentially- expressed genes in Np65-KO mice by microarray analysis. RT-PCR analysis also revealed the altered expression of genes associated with development and synaptic structure, such as Cdhl, Htr3a, and Kcnj9. In addition, the expression of Wnt-3, a Wnt protein involved in development, was decreased in Np65-KO mice as evidenced by western blotting. Surprisingly, MRI and DAPI staining showed a significant reduction in the lateral ventricular volume of Np65-KO mice. Together, these findings suggest that ablation of Np65 influences gene expression, which may contribute to abnormal brain development. These results provide clues to the mechanisms underlying the altered brain functions of Np65-deficient mice.

Au/g-C;N;heterostructure sensitized by black phosphorus for full solar spectrum waste-to-hydrogen conversion

Photocatalytic water splitting with simultaneous degradation of organic pollutants is an effective strategy to alleviate the increasingly serious energy and environmental crisis.However,the photocatalytic activity is restricted by the high charge recombination rate and limited sunlight utilization.Herein,black phosphorus(BP)with a broad sunlight response range was utilized as a photosensitizer to enhance the photocatalytic performance of the Au/carbon nitride(CN)heterostructure.The as-prepared BP/Au/CN exhibited a significantly enhanced H_(2) generation rate of 1400.8µmol h^(−1) g^(−1) under UV-vis light irradiation,which is almost 70 times higher than that of bare CN and BP/CN and 2 folds higher than that of the Au/CN heterojunction.Specifically,the optimal BP/Au/CN sample presented a waste-to-hydrogen production rate of 195.8µmol h^(−1) g^(−1) with the degradation of bisphenol A,verifying the synergistic effect of the ternary heterojunction.The photocatalytic mechanism was systematically studied by the combination of experiments and theoretical calculations.The improved photocatalytic performance was derived from the overall sunlight absorption ability of BP,effective electron transfer media and plasmonic character of Au nanoparticles,as well as the matched work function and strong interaction of the three components.A unidirectional electron transfer from BP to Au and then to CN was established,which effectively improved the charge transfer capability,resulting from the appropriate Ohmic contact of Au and BP and the Schottky barrier constructed in Au/CN hybrid.

Layered CuNi-Cu_(2)O/NiAlO_(x) nanocatalyst for rapid conversion of p-nitrophenol to p-aminophenol

In order to well arrange active sites and avoid byproducts, the reasonable structured carrier nanocatalyst plays a crucial role in high catalytic performance, but still remains a challenge. Herein, the layered CuNi-Cu_(2)O/NiAlO_(x) nanosheets have been constructed through hydrothermal synthesis followed by calcination and H_(2) reduction treatment process. The in-situ formed CuNi nanoalloys (NAs) and nano-Cu_(2)O were evenly distributed on the bilateral surface of layered NiAlOx nanosheets. Based on the planar structure of nanosheet, the synergy between catalytic active CuNi NAs and photocatalytic active nano-Cu_(2)O endows CuNi-Cu_(2)O/NiAlO_(x) nanosheets with rapid conversion efficiency for catalyzing p-nitrophenol (p-NP, 14 mg·L^(−1)) to p-aminophenol (p-AP) in 32 s with the reaction rate constant k up to 0.1779 s−1, and no obvious performance decay can be observed even over 27 cycles. Moreover, high concentration of p-NP at 10 and 20 g·L^(−1) could be reduced to p-AP within 14 and 20 min, respectively. Such designed nanoalloy/bimetal-oxide heterostructure can provide a solution for rapid conversion of aminoaromatics from nitroaromatics wastewater even at a large concentration range.

Synergistic CO_(2)reduction and tetracycline degradation by CuInZnS-Ti_(3)C_(2)T_(x)in one photoredox cycle

Optimizing photocatalytic CO_(2)reduction with simultaneous pollutant degradation is highly desired.However,the photocatalytic efficiency is restricted by the unmatched redox ability,high carriers’recombination rate,and lack of reactive sites of the present photocatalysts.Herein,the CuInZnS-Ti_(3)C_(2)T_(x)hybrid with matched redox ability and suitable CO_(2)adsorption property was rationally synthesized.The nucleation and growth process of CuInZnS was interfered by the addition of Ti_(3)C_(2)T_(x)with a negative charge,resulting in thinner nanosheets and richer reactive sites.Besides,the Schottky heterojunction built in the hybrid simultaneously improved the photoexcited charge transfer property,sunlight absorption range,and CO_(2)adsorption ability.Consequently,upon exposure to sunlight,CuInZnS-Ti_(3)C_(2)T_(x)exhibited an efficient photocatalytic CO_(2)reduction performance(10.2μmol·h^(−1)·g^(−1))with synergetic tetracycline degradation,obviously higher than that of pure CuInZnS.Based on the combination of theoretical calculation and experimental characterization,the photocatalytic mechanism was investigated comprehensively.This work offers a reference for the remission of worldwide energy shortage and environmental pollution problems.

An alternative splicing variant of mineralocorticoid receptor discovered in preeclampsia tissues and its effect on endothelial dysfunction

The pathophysiology of preeclampsia(PE)remains unclear.PE spiral artery remodeling dysfunction and PE offspring cardiovascular future development has been a worldwide concern.We collected placental and umbilical artery samples from normotensive and PE pregnancies.Mineralocorticoid receptor(MR)and its alternative splicing variant(ASV)expression and their biological effects on PE were examined.An MR ASV was found to be highly expressed in all PE samples and slightly expressed in about half of the normotensive samples(umbilical artery,~57.58%;placenta,~36.84%).The MR ASV expression was positively associated with blood pressure in both groups.The MR ASV protein changed the aldosterone-induced expression pattern of MR target genes related to ion exchanges and cell signaling pathways.The MR ASV can also impair the proliferation,migration,and tube formation ability of endothelial cells.These findings indicate that MR ASV in PE placenta plays a pathogenic role in PE pathophysiology,especially in endothelial dysfunction,and the existence of the MR ASV in PE umbilical artery provides a new direction in the study of PE offspring with increased risk of cardiovascular diseases.