Objective To validate the performance of Mycob. T Stainer and Scanner(MTSS) for detecting acid-fast bacilli(AFB). Methods A total of 3,816 sputum samples from 1,515 tuberculosis(TB) suspects were tested at the Anhui P...Objective To validate the performance of Mycob. T Stainer and Scanner(MTSS) for detecting acid-fast bacilli(AFB). Methods A total of 3,816 sputum samples from 1,515 tuberculosis(TB) suspects were tested at the Anhui Provincial Chest Hospital and the Linyi People’s Hospital from April-August, 2016. Each specimen was placed on two smear slides. One slide was stained by the ziehl-neelsen(ZN) method to be read by conventional microscopy(CM). The other slide was stained and scanned by MTSS. All specimens were decontaminated with 4% NaO H, and then inoculated into solid culture. The performance of MTSS was assessed. Results MTSS produced higher average positivity rate(27.96%) as compared with the CM(26.83%). The overall sensitivity and specificity of MTSS were 78.9% and 93.9%, respectively. The sensitivity and specificity of CM was 77.4% and 95.0%, respectively. Conclusion MTSS exhibited a favorable performance in the detection of AFB. It may be an alternative to CM for screening TB.展开更多
基金supported by Chinese Anti-Tuberculosis Association[042016]
文摘Objective To validate the performance of Mycob. T Stainer and Scanner(MTSS) for detecting acid-fast bacilli(AFB). Methods A total of 3,816 sputum samples from 1,515 tuberculosis(TB) suspects were tested at the Anhui Provincial Chest Hospital and the Linyi People’s Hospital from April-August, 2016. Each specimen was placed on two smear slides. One slide was stained by the ziehl-neelsen(ZN) method to be read by conventional microscopy(CM). The other slide was stained and scanned by MTSS. All specimens were decontaminated with 4% NaO H, and then inoculated into solid culture. The performance of MTSS was assessed. Results MTSS produced higher average positivity rate(27.96%) as compared with the CM(26.83%). The overall sensitivity and specificity of MTSS were 78.9% and 93.9%, respectively. The sensitivity and specificity of CM was 77.4% and 95.0%, respectively. Conclusion MTSS exhibited a favorable performance in the detection of AFB. It may be an alternative to CM for screening TB.
